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Literature summary for 2.7.9.1 extracted from
- Purification and characterization of recombinant pyruvate phosphate dikinase from Giardia (1999), Mol. Biochem. Parasitol., 104, 157-169.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in a baculovirus system | Giardia intestinalis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| biphosphonate | mixed inhibition mechanism with respect to diphosphate | Giardia intestinalis |  |
| diphosphate | - |
Giardia intestinalis | |
| Imidodiphosphate | competitive to diphosphate | Giardia intestinalis | |
| oxalate | mixed mechanism with respect to phosphoenolpyruvate and diphosphate | Giardia intestinalis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0013 | - |
AMP | pH 7, 30ºC | Giardia intestinalis | |
| 0.029 | - |
diphosphate | pH 7, 30ºC | Giardia intestinalis | |
| 0.032 | - |
ATP | pH 7, 30ºC | Giardia intestinalis | |
| 0.033 | - |
phosphoenolpyruvate | pH 7, 30ºC | Giardia intestinalis | |
| 0.075 | - |
pyruvate | pH 7, 30ºC | Giardia intestinalis | |
| 1.8 | - |
phosphate | pH 7, 30ºC | Giardia intestinalis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activity increases with increasing Mg2+ concentrations, reaching a plateau at 2-4 mM. At higher concentrations the effect begins to reverse | Giardia intestinalis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 97600 | - |
2 * 97600, analytical ultracentrifugation and gel filtration | Giardia intestinalis |
| 195000 | - |
gel filtration | Giardia intestinalis |
| 198400 | - |
gel electrophoresis | Giardia intestinalis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Giardia intestinalis | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + pyruvate + phosphate | - |
Giardia intestinalis | AMP + phosphoenolpyruvate + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 97600, analytical ultracentrifugation and gel filtration | Giardia intestinalis |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.001 | - |
oxalate | pH 7, 30ºC, less than 0.001 mM | Giardia intestinalis | |
| 0.55 | - |
diphosphate | pH 7, 30ºC | Giardia intestinalis | |
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Release 2023.1 (January 2023)
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