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Literature summary for 2.7.9.1 extracted from

  • Bringaud, F.; Baltz, D.; Baltz, T.
    Functional and molecular characterization of a glycosomal PPi-dependent enzyme in trypanosomatids: pyruvate, phosphate dikinase (1998), PROC. NATL. ACAD. SCI. USA, 95, 7963-7968.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Trypanosoma brucei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0075
-
AMP
-
Trypanosoma brucei
0.04
-
phosphoenolpyruvate
-
Trypanosoma brucei
0.05
-
diphosphate
-
Trypanosoma brucei
0.3
-
pyruvate
-
Trypanosoma brucei
0.5
-
phosphate
-
Trypanosoma brucei
0.6
-
ATP
-
Trypanosoma brucei

Localization

Localization Comment Organism GeneOntology No. Textmining
glycosome
-
Trypanosoma brucei 20015
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ not required for activity Trypanosoma brucei
Mg2+ required for activity Trypanosoma brucei
NH4+ not required for activity Trypanosoma brucei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
antibody recognition Trypanosoma cruzi
100000
-
antibody recognition Leishmania sp.
100000
-
antibody recognition Trypanosoma congolense
100000
-
antibody recognition Trypanosoma brucei
100000
-
antibody recognition Trypanosoma vivax
100000
-
antibody recognition Crithidia sp.
100000
-
antibody recognition Phytomonas sp.

Organism

Organism UniProt Comment Textmining
Crithidia sp.
-
-
-
Leishmania sp.
-
-
-
Phytomonas sp.
-
-
-
Trypanosoma brucei O76283
-
-
Trypanosoma congolense
-
-
-
Trypanosoma cruzi
-
-
-
Trypanosoma vivax
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5
-
catalysis of both the pyruvate and the phosphoenolpyruvate formation Trypanosoma brucei

pI Value

Organism Comment pI Value Maximum pI Value
Trypanosoma brucei calculated
-
8.25