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Literature summary for 2.7.9.1 extracted from

  • Schwitzguebel, J.P.; Ettlinger, L.
    Pyruvate, orthophosphate dikinase from Acetobacter aceti (1979), Arch. Microbiol., 122, 103-108.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
AMP
-
Acetobacter aceti
ATP competitive to AMP Acetobacter aceti
diphosphate competitive to phosphate Acetobacter aceti
phosphate competitive to diphosphate Acetobacter aceti
phosphoenolpyruvate competitive to pyruvate Acetobacter aceti
pyruvate competitive to phosphoenolpyruvate Acetobacter aceti

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
AMP pH 7.1, 30ºC, pyruvate formation Acetobacter aceti
0.027
-
pyruvate pH 8, 30ºC, phosphoenolpyruvate formation Acetobacter aceti
0.062
-
diphosphate pH 7.1, 30ºC, pyruvate formation Acetobacter aceti
0.13
-
phosphoenolpyruvate pH 7.1, 30ºC, pyruvate formation Acetobacter aceti
0.2
-
ATP pH 8, 30ºC, phosphoenolpyruvate formation Acetobacter aceti
0.83
-
phosphate pH 8, 30ºC, phosphoenolpyruvate formation Acetobacter aceti

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ cannot be replaced by other divalent ions Acetobacter aceti

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
6 * 58000, SDS-PAGE Acetobacter aceti
320000
-
gel filtration on Sephadex G-200 Acetobacter aceti
330000
-
gel filtration on Sephrose 6B Acetobacter aceti

Organism

Organism UniProt Comment Textmining
Acetobacter aceti
-
-
-

Purification (Commentary)

Purification (Comment) Organism
using hydroxyapatite, Sephadex G-200 and DEAE column chromatography Acetobacter aceti

Reaction

Reaction Comment Organism Reaction ID
ATP + pyruvate + phosphate = AMP + phosphoenolpyruvate + diphosphate non-classical three-site tri ping-pong kinetics Acetobacter aceti

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.9
-
-
Acetobacter aceti

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + pyruvate + phosphate
-
Acetobacter aceti AMP + phosphoenolpyruvate + diphosphate
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 58000, SDS-PAGE Acetobacter aceti

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Acetobacter aceti

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1
-
pyruvate formation Acetobacter aceti
8
-
phosphoenolpyruvate formation Acetobacter aceti

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.32
-
AMP pH 8, 30ºC, phosphoenolpyruvate formation Acetobacter aceti
0.36
-
diphosphate pH 8, 30ºC, phosphoenolpyruvate formation Acetobacter aceti
0.6
-
ATP pH 7.1, 30ºC, pyruvate formation Acetobacter aceti
0.76
-
pyruvate pH 7.1, 30ºC, pyruvate formation Acetobacter aceti
2
-
phosphoenolpyruvate pH 8, 30ºC, phosphoenolpyruvate formation Acetobacter aceti
6
-
phosphate pH 7.1, 30ºC, pyruvate formation Acetobacter aceti