Literature summary for 2.7.8.B11 extracted from

  • Tan, B.K.; Bogdanov, M.; Zhao, J.; Dowhan, W.; Raetz, C.R.; Guan, Z.
    Discovery of a cardiolipin synthase utilizing phosphatidylethanolamine and phosphatidylglycerol as substrates (2012), Proc. Natl. Acad. Sci. USA, 109, 16504-16509.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
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Escherichia coli

Engineering

Protein Variants Comment Organism
additional information Escherichia coli has three cardiolipin synthases. Cardiolipin synthase A encoded by clsA, cardiolipin synthase B encoded by clsB and cardiolipin synthase C, encoded by clsC. Deletion of all three genes (DELTAclsABC) results in a complete lack of cardiolipin. Restoration of near–wild-type levels of cardiolipin production in the stationary phase by expression of clsC in the triple mutant requires the coexpression from the same operon of the preceding gene ymdB. The mode of cardiolipin biosynthesis by the combined YmdB-ClsC follows neither the “eukaryotic” nor “prokaryotic” pathway but involves the transfer of a phosphatidyl moiety from PE to the terminal free hydroxyl of PG to form cardiolipin Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P75919 cardiolipin synthase C
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-(1-heptadecanoyl-2-((9Z)-tetradec-9-enoyl)-sn-glycero-3-phospho)-sn-glycerol + 1-dodecanoyl-2-tridecanoyl-sn-glycero-3-phosphoethanolamine synthetic phosphatidylglycerol (17:0/14:1) and synthetic phosphatidylethanolamine (12:0/13:0). Reaction is catalyzed by the two-component complex YmdB-ClsC. Escherichia coli has three cardiolipin synthases. Cardiolipin synthase A encoded by clsA, cardiolipin synthase B encoded by clsB and cardiolipin synthase C, encoded by clsC. Deletion of all three genes (DELTAclsABC) results in a complete lack of cardiolipin. Restoration of near–wild-type levels of cardiolipin production in the stationary phase by expression of clsC in the triple mutant requires the coexpression from the same operon of the preceding gene ymdB. The mode of cardiolipin biosynthesis by the combined YmdB-ClsC follows neither the “eukaryotic” nor “prokaryotic” pathway but involves the transfer of a phosphatidyl moiety from phosphatidylethanolamine to the terminal free hydroxyl of phosphatidylglycerol to form cardiolipin Escherichia coli 1-(1-heptadecanoyl-2-((9Z)-tetradec-9-enoyl)-sn-glycero-3-phospho)-3-(1-dodecanoyl-2-tridecanoyl-sn-glycero-3-phospho)glycerol + ethanolamine
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Subunits

Subunits Comment Organism
More reaction is catalyzed by the two-component complex YmdB-ClsC Escherichia coli

Synonyms

Synonyms Comment Organism
clsC
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Escherichia coli

General Information

General Information Comment Organism
physiological function Escherichia coli has three cardiolipin synthases. All cardiolipin synthases contribute cardiolipin the stationary phase. Only cardiolipin synthase A makes detectible cardiolipin in low-osmolarity medium during logarithmic growth and contributes the majority of cardiolipin in high-osmolarity medium Escherichia coli
malfunction Escherichia coli has three cardiolipin synthases. Cardiolipin synthase A encoded by clsA, cardiolipin synthase B encoded by clsB and cardiolipin synthase C, encoded by clsC. Triple deletions of clsA, clsB, and clsC results in the complete depletion of cardiolipin synthase activity in Escherichia coli cells. The double mutant DELTAclsAB mutant still makes cardiolipin in the stationary phase Escherichia coli