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Literature summary for 2.7.8.B10 extracted from

  • Liu, J.; Ryabichko, S.; Bogdanov, M.; Fackelmayer, O.J.; Dowhan, W.; Krulwich, T.A.
    Cardiolipin is dispensable for oxidative phosphorylation and non-fermentative growth of alkaliphilic Bacillus pseudofirmus OF4 (2014), J. Biol. Chem., 289, 2960-2971.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.8.B10

Cloned(Commentary)

Cloned (Comment) Organism
three putative Cl synthase encoding genes: clsA, clsB, and clsC Alkalihalophilus pseudofirmus

Protein Variants

Protein Variants Comment Organism
additional information construction of three single, DELTAclsA, DELTAclsB, and DELTAclsC, one double, DELTAclsA/B, and one triple, DELTAclsA/B/C, mutants Alkalihalophilus pseudofirmus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Alkalihalophilus pseudofirmus 16020
-

Organism

Organism UniProt Comment Textmining
Alkalihalophilus pseudofirmus O66043 and methionine auxotroph derivative strain 811M
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Alkalihalophilus pseudofirmus OF4 O66043 and methionine auxotroph derivative strain 811M
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Synonyms

Synonyms Comment Organism
CL synthase
-
 Alkalihalophilus pseudofirmus
CLS
-
 Alkalihalophilus pseudofirmus
clsA
-
 Alkalihalophilus pseudofirmus
clsB
-
 Alkalihalophilus pseudofirmus
clsC
-
 Alkalihalophilus pseudofirmus

General Information

General Information Comment Organism
malfunction no detectablecardiolipin is found in DELTAclsA mutants, whereas the cardiolipin precursor phosphatidylglycerol is elevated. The DELTAclsB mutant exhibits no significant reduction in cardiolipin, but at pH 7.5, clsB expression is upregulated and appears growth. In the absence of detectable cardiolipin, the alkaliphile shows no significant deficits in non-fermentative growth, respiration-dependent ATP synthesis, or salt tolerance. In long term survival experiments, significant growth defects are found in DELTAclsA strains and the DELTAclsC strain at pH10.5. Both the single DELTAclsA and triple deletion mutants have a significant reduction in NADH dehydrogenase activity Alkalihalophilus pseudofirmus
metabolism the clsA gene plays a major role in cardiolipin biosynthesis Alkalihalophilus pseudofirmus
physiological function cardiolipin is not essential for oxidative phosphorylation and the non-fermentative growth it supports in alkaliphilic Bacillus pseudofirmus strain OF4, during growth at either pH 7.5 or pH 10.5. Cardiolipin does not seem be a crucial participant in the proton movements on or near the membrane surface from proton-pumping respiratory chain complexes to the F1F0-ATP synthase complex Alkalihalophilus pseudofirmus