Cloned (Comment) | Organism |
---|---|
gene mclS, DNA and amino acid sequence determination and analysis of the gene sequences from isolates O12E and McGHS1. The mutant strains lack cardiolipin production | Moraxella catarrhalis |
Protein Variants | Comment | Organism |
---|---|---|
K438R | a lipolytically inactive form of mclS, O35E.mclS.K438R | Moraxella catarrhalis |
additional information | construction of mclS knockout strains from wild-type Moraxella catarrhalis isolates O35E, O12E, and McGHS1 yielding isogenic mutant strains O35E.mclS, O12E.mclS, and McGHS1.mclS. Wild-type and mutant strains show no differences in growth behaviour | Moraxella catarrhalis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Moraxella catarrhalis | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
a phosphatidylglycerol + a phosphatidylglycerol | Moraxella catarrhalis | - |
a cardiolipin + glycerol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Moraxella catarrhalis | R4V164 | isolates O35E, O12E, and McGHS1 | - |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a phosphatidylglycerol + a phosphatidylglycerol | - |
Moraxella catarrhalis | a cardiolipin + glycerol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cardiolipin synthase | - |
Moraxella catarrhalis |
mclS | - |
Moraxella catarrhalis |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a member of the PLD superfamily of proteins, the enzyme is conserved among Moraxella catarrhalis isolates | Moraxella catarrhalis |
malfunction | cardiolipin-deficient Moraxella catarrhalis exhibits wild-type growth in complete medium and under osmotic stress | Moraxella catarrhalis |
physiological function | enzyme MclS is responsible for the synthesis of cardiolipin, which is a major component of the Moraxella catarrhalis cell envelope, constituting 5-30% of the total membrane phospholipid. The mclS gene product is not required for optimal growth of Moraxella catarrhalis in vitro. Expression of the mclS gene product impacts the adherence of Moraxella catarrhalis to human epithelial cells. The contribution of mclS to adherence is indirect, possibly by modulating the proper surface display of Moraxella catarrhalis adhesins on the bacterial surface through its CLS activity | Moraxella catarrhalis |