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Literature summary for 2.7.8.B10 extracted from

  • Buskirk, S.W.; Lafontaine, E.R.
    Moraxella catarrhalis expresses a cardiolipin synthase that impacts adherence to human epithelial cells (2014), J. Bacteriol., 196, 107-120.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene mclS, DNA and amino acid sequence determination and analysis of the gene sequences from isolates O12E and McGHS1. The mutant strains lack cardiolipin production Moraxella catarrhalis

Protein Variants

Protein Variants Comment Organism
K438R a lipolytically inactive form of mclS, O35E.mclS.K438R Moraxella catarrhalis
additional information construction of mclS knockout strains from wild-type Moraxella catarrhalis isolates O35E, O12E, and McGHS1 yielding isogenic mutant strains O35E.mclS, O12E.mclS, and McGHS1.mclS. Wild-type and mutant strains show no differences in growth behaviour Moraxella catarrhalis

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
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Moraxella catarrhalis 16020
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
a phosphatidylglycerol + a phosphatidylglycerol Moraxella catarrhalis
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a cardiolipin + glycerol
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?

Organism

Organism UniProt Comment Textmining
Moraxella catarrhalis R4V164 isolates O35E, O12E, and McGHS1
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Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
a phosphatidylglycerol + a phosphatidylglycerol
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Moraxella catarrhalis a cardiolipin + glycerol
-
?

Synonyms

Synonyms Comment Organism
cardiolipin synthase
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Moraxella catarrhalis
mclS
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Moraxella catarrhalis

General Information

General Information Comment Organism
evolution the enzyme is a member of the PLD superfamily of proteins, the enzyme is conserved among Moraxella catarrhalis isolates Moraxella catarrhalis
malfunction cardiolipin-deficient Moraxella catarrhalis exhibits wild-type growth in complete medium and under osmotic stress Moraxella catarrhalis
physiological function enzyme MclS is responsible for the synthesis of cardiolipin, which is a major component of the Moraxella catarrhalis cell envelope, constituting 5-30% of the total membrane phospholipid. The mclS gene product is not required for optimal growth of Moraxella catarrhalis in vitro. Expression of the mclS gene product impacts the adherence of Moraxella catarrhalis to human epithelial cells. The contribution of mclS to adherence is indirect, possibly by modulating the proper surface display of Moraxella catarrhalis adhesins on the bacterial surface through its CLS activity Moraxella catarrhalis