BRENDA - Enzyme Database
show all sequences of 2.7.8.8

Ribosomal-associated phosphatidylserine synthetase from Escherichia coli: purification by substrate-specific elution from phosphocellulose using cytidine 5-diphospho-1,2-diacyl-sn-glycerol

Larson, T.J.; Dowhan, W.; Biochemistry 15, 5212-5218 (1976)

Data extracted from this reference:

General Stability
General Stability
Organism
high ionic strength buffers are neceassary to prevent irreversible precipitation and inactivation of the enzyme
Escherichia coli
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
ribosome
tightly bound to ribosome
Escherichia coli
5840
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
54000
-
x * 54000, SDS-PAGE
Escherichia coli
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Purification (Commentary)
Commentary
Organism
-
Escherichia coli
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
33
-
-
Escherichia coli
Storage Stability
Storage Stability
Organism
-20°C, stable for ar least 1 month
Escherichia coli
4°C, 25% loss after 1 months
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
CDP-diacylglycerol + glycerol
low activity
643776
Escherichia coli
CMP + phosphatidylglycerol
-
643776
Escherichia coli
?
CDP-diacylglycerol + H2O
-
643776
Escherichia coli
CMP + phosphatidic acid
-
643776
Escherichia coli
?
CDP-diacylglycerol + L-Ser
-
643776
Escherichia coli
CMP + 3-O-sn-phosphatidyl-L-serine
-
643776
Escherichia coli
?
CDP-diacylglycerol + sn-glycero-3-phosphate
low activity
643776
Escherichia coli
CMP + phosphatidylglycerophosphate
-
643776
Escherichia coli
?
additional information
the enzyme also catalyzes the exchange reaction between Ser and phosphatidylserine
643776
Escherichia coli
?
-
-
-
-
additional information
enzyme catalyzes exchange reaction between dCDP-diglyceride and dCDP-diglyceride
643776
Escherichia coli
?
-
-
-
-
additional information
enzyme catalyzes exchange reaction between CMP and CDP-diglyceride
643776
Escherichia coli
?
-
-
-
-
phosphatidylserine + H2O
-
643776
Escherichia coli
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 54000, SDS-PAGE
Escherichia coli
General Stability (protein specific)
General Stability
Organism
high ionic strength buffers are neceassary to prevent irreversible precipitation and inactivation of the enzyme
Escherichia coli
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
ribosome
tightly bound to ribosome
Escherichia coli
5840
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
54000
-
x * 54000, SDS-PAGE
Escherichia coli
Purification (Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
33
-
-
Escherichia coli
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, stable for ar least 1 month
Escherichia coli
4°C, 25% loss after 1 months
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
CDP-diacylglycerol + glycerol
low activity
643776
Escherichia coli
CMP + phosphatidylglycerol
-
643776
Escherichia coli
?
CDP-diacylglycerol + H2O
-
643776
Escherichia coli
CMP + phosphatidic acid
-
643776
Escherichia coli
?
CDP-diacylglycerol + L-Ser
-
643776
Escherichia coli
CMP + 3-O-sn-phosphatidyl-L-serine
-
643776
Escherichia coli
?
CDP-diacylglycerol + sn-glycero-3-phosphate
low activity
643776
Escherichia coli
CMP + phosphatidylglycerophosphate
-
643776
Escherichia coli
?
additional information
the enzyme also catalyzes the exchange reaction between Ser and phosphatidylserine
643776
Escherichia coli
?
-
-
-
-
additional information
enzyme catalyzes exchange reaction between dCDP-diglyceride and dCDP-diglyceride
643776
Escherichia coli
?
-
-
-
-
additional information
enzyme catalyzes exchange reaction between CMP and CDP-diglyceride
643776
Escherichia coli
?
-
-
-
-
phosphatidylserine + H2O
-
643776
Escherichia coli
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 54000, SDS-PAGE
Escherichia coli
Other publictions for EC 2.7.8.8
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739446
Ma
Phosphatidylserine synthase co ...
Oryza sativa Japonica Group
PLoS ONE
11
e0153119
2016
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2
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723504
Yin
SUI-family genes encode phosph ...
Oryza sativa
Planta
237
15-27
2013
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738817
Liu
Phosphatidylserine synthase 1 ...
Arabidopsis thaliana
J. Integr. Plant Biol.
55
682-695
2013
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2
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722905
Morita
Enzymatic measurement of phosp ...
Homo sapiens
J. Lipid Res.
53
325-330
2012
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723425
Yamaoka
Phosphatidylserine synthase1 i ...
Arabidopsis thaliana
Plant J.
67
648-661
2011
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8
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1
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1
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705791
Chen
Phosphatidylserine synthase an ...
Candida albicans
Mol. Microbiol.
75
1112-1132
2010
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704125
Zhang
Expression, purification, and ...
Escherichia coli
J. Agric. Food Chem.
57
122-126
2009
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5
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1
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4
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1
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661745
Linde
Lipid dependence and activity ...
Escherichia coli
FEBS Lett.
575
77-80
2004
3
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692823
Morii
CDP-2,3-di-O-geranylgeranyl-sn ...
Escherichia coli
J. Bacteriol.
185
1181-1189
2003
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643796
Rilfors
Reconstituted phosphatidylseri ...
Escherichia coli
Biochim. Biophys. Acta
1438
281-294
1999
1
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643797
Matsumoto
Phosphatidylserine synthase fr ...
Bacillus subtilis, Escherichia coli
Biochim. Biophys. Acta
1348
214-227
1997
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643779
Dowhan
Phosphatidylserine synthase fr ...
Escherichia coli
Methods Enzymol.
209
287-298
1992
1
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643789
Raetz
Phospholipids chiral at phosph ...
Escherichia coli
Biochemistry
26
4022-4027
1987
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643791
Louie
Substrate-induced membrane ass ...
Escherichia coli
J. Bacteriol.
165
805-812
1986
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643778
Dowhan
-
Phosphatidylserine synthase fr ...
Escherichia coli
Methods Enzymol.
71
561-571
1981
2
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643782
Carman
Phosphatidylserine synthase fr ...
Escherichia coli
J. Biol. Chem.
254
8391-8397
1979
1
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643776
Larson
Ribosomal-associated phosphati ...
Escherichia coli
Biochemistry
15
5212-5218
1976
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643777
Raetz
-
Partial purification and prope ...
Escherichia coli
J. Biol. Chem.
249
5038-5045
1974
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643784
Ishinaga
Effects of phospholipids on so ...
Escherichia coli
FEBS Lett.
49
201-202
1974
2
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643781
Raetz
The association of phosphatidy ...
Escherichia coli
J. Biol. Chem.
247
2008-2014
1972
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