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Literature summary for 2.7.8.8 extracted from
- Ribosomal-associated phosphatidylserine synthetase from Escherichia coli: purification by substrate-specific elution from phosphocellulose using cytidine 5-diphospho-1,2-diacyl-sn-glycerol (1976), Biochemistry, 15, 5212-5218.
General Stability
| General Stability | Organism |
|---|---|
| high ionic strength buffers are neceassary to prevent irreversible precipitation and inactivation of the enzyme | Escherichia coli |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| ribosome | tightly bound to ribosome | Escherichia coli | 5840 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
x * 54000, SDS-PAGE | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 33 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for ar least 1 month | Escherichia coli |
| 4°C, 25% loss after 1 months | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CDP-diacylglycerol + glycerol | low activity | Escherichia coli | CMP + phosphatidylglycerol | - |
? |  |
| CDP-diacylglycerol + H2O | - |
Escherichia coli | CMP + phosphatidic acid | - |
? | |
| CDP-diacylglycerol + L-Ser | - |
Escherichia coli | CMP + 3-O-sn-phosphatidyl-L-serine | - |
? | |
| CDP-diacylglycerol + sn-glycero-3-phosphate | low activity | Escherichia coli | CMP + phosphatidylglycerophosphate | - |
? | |
| additional information | the enzyme also catalyzes the exchange reaction between Ser and phosphatidylserine | Escherichia coli | ? | - |
? | |
| additional information | enzyme catalyzes exchange reaction between dCDP-diglyceride and dCDP-diglyceride | Escherichia coli | ? | - |
? | |
| additional information | enzyme catalyzes exchange reaction between CMP and CDP-diglyceride | Escherichia coli | ? | - |
? | |
| phosphatidylserine + H2O | - |
Escherichia coli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 54000, SDS-PAGE | Escherichia coli |
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Release 2023.1 (January 2023)
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