Application | Comment | Organism |
---|---|---|
molecular biology | SchPPT is a promiscuous PPTase and may be used on polyketide production in heterologous bacterial host and labeling of acyl-carrier proteins, ACPs | Streptomyces chattanoogensis |
Cloned (Comment) | Organism |
---|---|
gene acpS, recombinant expression of N-terminaly His6-tagged enzyme in Escherichia coli BL21(DE3) from plasmid pHJ0024 | Streptomyces chattanoogensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Streptomyces chattanoogensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] | Streptomyces chattanoogensis | PPTases transfer 4'-phosphopantetheine from coenzyme A (CoA) to highly conserved serine residues in PCPs/ACPs, converting PCPs/ACPs from inactive apo-forms into active holo-forms | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
? | |
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] | Streptomyces chattanoogensis L10 | PPTases transfer 4'-phosphopantetheine from coenzyme A (CoA) to highly conserved serine residues in PCPs/ACPs, converting PCPs/ACPs from inactive apo-forms into active holo-forms | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces chattanoogensis | H9N839 | - |
- |
Streptomyces chattanoogensis L10 | H9N839 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminaly His6-tagged enzyme from Escherichia coli BL21(DE3) by nickel affinity chromatography | Streptomyces chattanoogensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] | PPTases transfer 4'-phosphopantetheine from coenzyme A (CoA) to highly conserved serine residues in PCPs/ACPs, converting PCPs/ACPs from inactive apo-forms into active holo-forms | Streptomyces chattanoogensis | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
? | |
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] | usage of ACP substrate kbB-ACP4, an ACP in FK506 biosynthetic PKS/NRPS hybrid from Streptomyces tsukubaensis | Streptomyces chattanoogensis | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
? | |
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] | PPTases transfer 4'-phosphopantetheine from coenzyme A (CoA) to highly conserved serine residues in PCPs/ACPs, converting PCPs/ACPs from inactive apo-forms into active holo-forms | Streptomyces chattanoogensis L10 | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
? | |
CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] | usage of ACP substrate kbB-ACP4, an ACP in FK506 biosynthetic PKS/NRPS hybrid from Streptomyces tsukubaensis | Streptomyces chattanoogensis L10 | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
? | |
additional information | substrate promiscuity of the phosphopantetheinyl transferase SchPPT for coenzyme A derivatives and acyl carrier proteins, SchPPT has a broad substrate specificity for ACPs | Streptomyces chattanoogensis | ? | - |
? | |
additional information | substrate promiscuity of the phosphopantetheinyl transferase SchPPT for coenzyme A derivatives and acyl carrier proteins, SchPPT has a broad substrate specificity for ACPs | Streptomyces chattanoogensis L10 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AcpS | - |
Streptomyces chattanoogensis |
group II PPTase | - |
Streptomyces chattanoogensis |
holo-ACP synthase | UniProt | Streptomyces chattanoogensis |
holo-[acyl-carrier-protein] synthase | UniProt | Streptomyces chattanoogensis |
phosphopantetheinyl transferase | - |
Streptomyces chattanoogensis |
PPTase | - |
Streptomyces chattanoogensis |
SchPPT | - |
Streptomyces chattanoogensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Streptomyces chattanoogensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Streptomyces chattanoogensis |
General Information | Comment | Organism |
---|---|---|
evolution | SchPPT is a group II PPTase | Streptomyces chattanoogensis |
physiological function | phosphopantetheinyl transferases play an essential role in the biosyntheses of fatty acids, polyketides, and nonribosomal peptides | Streptomyces chattanoogensis |