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Literature summary for 2.7.8.7 extracted from

  • Flaman, A.S.; Chen, J.M.; Van Iderstine, S.C.; Byers, D.M.
    Site-directed mutagenesis of acyl carrier protein (ACP) reveals amino acid residues involved in ACP structure and acyl-ACP synthetase activity (2001), J. Biol. Chem., 276, 35934-35939.
    View publication on PubMed
Search for more literature for 2.7.8.7

Application

Application Comment Organism
biotechnology mutant ACPs will be valuable in dissecting the structure-function relationships of ACP and its participation in synthesis and regulation of fatty acid, phospholipids, and other products of medical and biotechnological importance Vibrio harveyi

Cloned(Commentary)

Cloned (Comment) Organism
acpP gene amplified from a genomic Vibrio harveyi library by PCR, cloned and transformed in to Escherichia coli Bl-21 Vibrio harveyi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 apo-acyl-carrier protein pH 7.8, 37°C, mutant acyl carrier protein Y71A Vibrio harveyi
0.0073
-
 apo-acyl-carrier protein pH 7.8, 37°C, mutant acyl carrier protein V12G Vibrio harveyi
0.0079
-
 apo-acyl-carrier protein pH 7.8, 37°C Vibrio harveyi
0.0087
-
 apo-acyl-carrier protein pH 7.8, 37°C, r acyl carrier protein Vibrio harveyi
0.0094
-
 apo-acyl-carrier protein pH 7.8, 37°C, Escherichia coli acyl carrier protein Vibrio harveyi
0.013
-
 apo-acyl-carrier protein pH 7.8, 37°C, mutant acyl carrier protein A59G Vibrio harveyi
0.017
-
 apo-ACP pH 7.8, 37°C, mutant acyl carrier protein F50A Vibrio harveyi
0.029
-
 apo-acyl-carrier protein pH 7.8, 37°C, mutant acyl carrier protein I54L Vibrio harveyi
0.035
-
 apo-ACP pH 7.8, 37°C, mutant ACP I54V Vibrio harveyi

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Vibrio harveyi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CoA + apo-[acyl-carrier protein] Vibrio harveyi
-
 adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 r

Organism

Organism UniProt Comment Textmining
Vibrio harveyi P0A2W3 acpP gene
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CoA + apo-[acyl-carrier protein]
-
 Vibrio harveyi adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 r
additional information Escherichia coli ACP and mutant ACP proteins rACP, V12G, F50A, I54L, I154V, A59G and Y71A are substrates, mutant I54A is no substrate Vibrio harveyi ?
-
 ?