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Literature summary for 2.7.8.7 extracted from

  • Quadri, L.E.; Weinreb, P.H.; Lei, M.; Nakano, M.M.; Zuber, P.; Walsh, C.T.
    Characterization of Sfp, a Bacillus subtilis phosphopantetheinyl transferase for peptidyl carrier protein domains in peptide synthetases (1998), Biochemistry, 37, 1585-1595.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloned in Escherichia coli DH5alpha and overexpressed in Escherichia coli MV1190/pUC8-Sfp Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
D107A site-directed mutagenesis of the sfp gene, constructed using the SOE method Bacillus subtilis
D107E site-directed mutagenesis of the sfp gene, constructed using the SOE method Bacillus subtilis
E151A site-directed mutagenesis of the sfp gene, constructed using the SOE method Bacillus subtilis
G105A site-directed mutagenesis of the sfp gene, constructed using the SOE method Bacillus subtilis
G105D site-directed mutagenesis of the sfp gene, constructed using the SOE method Bacillus subtilis
K155A site-directed mutagenesis of the sfp gene, constructed using the SOE method Bacillus subtilis
W147A site-directed mutagenesis of the sfp gene, constructed using the SOE method Bacillus subtilis
W147F site-directed mutagenesis of the sfp gene, constructed using the SOE method Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
additional information no substantial inhibition of Sfp by apo-[PCPH6SrfB1.18] Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0018
-
apo-[PCPH6SrfB2.18] pH 6.0, 37°C Bacillus subtilis
0.005
-
apo-[Lys2-PCP-H6 Saccharomyces cerevisiae] pH 6.0, 37°C Bacillus subtilis
0.006
-
apo-acyl-carrier protein pH 6.0, 37°C Bacillus subtilis
0.007
-
CoA pH 6.0, 37°C Bacillus subtilis
0.016
-
apo-[EntB-ArCP-H6 Escherichia coli] pH 6.0, 37°C Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CoA + apo-[acyl-carrier protein] Bacillus subtilis enzyme Sfp required for production of the lipoheptapeptide antibiotic surfactin adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
r

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme, expressed in Escherichia coli MV1190/pUC8-Sfp Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetonyldethio-CoA + apo-[acyl-carrier protein]
-
Bacillus subtilis ? + holo-[acyl-carrier protein]
-
r
acetyl-CoA + apo-[acyl-carrier protein]
-
Bacillus subtilis CoA + acetyl-[acyl-carrier protein]
-
r
benzoyl-CoA + apo-[acyl-carrier protein]
-
Bacillus subtilis CoA + benzoyl-[acyl-carrier protein]
-
r
CoA + apo-[acyl-carrier protein]
-
Bacillus subtilis adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
r
CoA + apo-[acyl-carrier protein] enzyme Sfp required for production of the lipoheptapeptide antibiotic surfactin Bacillus subtilis adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
r
CoA + apo-[EntB-ArCP-H6 E. coli]
-
Bacillus subtilis ? + holo-[acyl-carrier protein]
-
r
CoA + apo-[Lys2-PCP-H6 Saccharomyces cerevisiae]
-
Bacillus subtilis ? + holo-[acyl-carrier protein]
-
r
CoA + apo-[PCPH6SrfB1.18]
-
Bacillus subtilis ? + holo-[acyl-carrier protein]
-
r
CoA + apo-[PCPH6SrfB2.18]
-
Bacillus subtilis ? + holo-[acyl-carrier protein]
-
r
CoA-[4'-phosphopantetheine] + apo-[EntB-ArCP-H6 Escherichia coli]
-
Bacillus subtilis ?
-
?
desulfo-CoA + apo-[acyl-carrier protein]
-
Bacillus subtilis ? + holo-[acyl-carrier protein]
-
r
homocysteamine-CoA + apo-[acyl-carrier protein]
-
Bacillus subtilis ? + holo-[acyl-carrier protein]
-
r
additional information 3'-dephospho-CoA is no substrate Bacillus subtilis ?
-
?
phenylacetyl-CoA + apo-[acyl-carrier protein]
-
Bacillus subtilis ? + holo-[acyl-carrier protein]
-
r

Synonyms

Synonyms Comment Organism
phosphopantetheinyl transferase
-
Bacillus subtilis
PPTase
-
Bacillus subtilis
Sfp
-
Bacillus subtilis
surfactin synthetase
-
Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0967
-
apo-acyl-carrier protein pH 6.0, 33°C Bacillus subtilis
0.933
-
apo-[PCPH6SrfB2.18] pH 6.0, 37°C Bacillus subtilis
1.08
-
apo-[EntB-ArCP-H6 Escherichia coli] pH 6.0, 37°C Bacillus subtilis
1.17
-
apo-[Lys2-PCP-H6 Saccharomyces cerevisiae] pH 6.0, 37°C Bacillus subtilis
1.7
-
apo-acyl-carrier protein pH 6.0, 37°C Bacillus subtilis
1.73
-
apo-[PCPH6SrfB1.18] pH 6.0, 37°C Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Bacillus subtilis

pH Range

pH Minimum pH Maximum Comment Organism
4.5 8.5 less than 20% of maximum activity at both pH 5.0 and 7.0, 50% of activity maximum at pH 5.5 and pH 6.8 Bacillus subtilis