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Literature summary for 2.7.8.5 extracted from
- Phosphatidylglycerophosphate synthase from Escherichia coli (1981), Methods Enzymol., 71, 555-561.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | 0.2% | Escherichia coli |  |
| Triton X-100 | maximal activity is dependent on Triton X-100 | Escherichia coli | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CDP-diacylglycerol | uncompetitive at high concentration | Escherichia coli | |
| Mg2+ | required, Km: 50 mM, inhibition above 150 mM | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.034 | - |
dCDP-diacylglycerol | 37°C, pH 8.0 | Escherichia coli | |
| 0.046 | - |
CDP-diacylglycerol | 37°C, pH 8.0 | Escherichia coli | |
| 0.32 | - |
sn-glycerol 3-phosphate | pH 8.0, 37°C | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | absolute requirement for a divalent metal, Mg2+ required, Km: 50 mM, inhibition above 150 mM | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24000 | - |
x * 24000, SDS-PAGE | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| CDP-diacylglycerol + sn-glycerol 3-phosphate = CMP + 1-(3-sn-phosphatidyl)-sn-glycerol 3-phosphate | ordered sequential bi-bi reaction | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 18.6 | - |
37°C, pH 8.0 | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CDP-diacylglycerol + sn-glycero-3-phosphate | - |
Escherichia coli | CMP + phosphatidylglycerophosphate | - |
? | |
| dCDP-diacylglycerol + sn-glycero-3-phosphate | - |
Escherichia coli | dCMP + phosphatidylglycerophosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 24000, SDS-PAGE | Escherichia coli |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
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