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Literature summary for 2.7.8.43 extracted from
- Functional genomics to discover antibiotic resistance genes: the paradigm of resistance to colistin mediated by ethanolamine phosphotransferase in Shewanella algae MARS 14 (2015), Int. J. Antimicrob. Agents, 46, 648-652.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene eptA, construction of a pZE21MCS-1 plasmid-based genomic expression library in Escherichia coli strain TOP10, DNA and amino acid sequence determination and analysis, functional EptA enzyme expression in Escherichia coli strain TOP10 | Shewanella algae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diacylphosphatidylethanolamine + lipid A | Shewanella algae | - |
diacylglycerol + lipid A (2-aminoethyl diphosphate) | - |
? |  |
| diacylphosphatidylethanolamine + lipid A | Shewanella algae MARS 14 | - |
diacylglycerol + lipid A (2-aminoethyl diphosphate) | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Shewanella algae | A0A0H5BY27 | a colistin-resistant clinical isolate from resistant clinical isolate retrieved from bronchoalveolar lavage of a hospitalised patient | - |
| Shewanella algae MARS 14 | A0A0H5BY27 | a colistin-resistant clinical isolate from resistant clinical isolate retrieved from bronchoalveolar lavage of a hospitalised patient | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diacylphosphatidylethanolamine + lipid A | - |
Shewanella algae | diacylglycerol + lipid A (2-aminoethyl diphosphate) | - |
? | |
| diacylphosphatidylethanolamine + lipid A | - |
Shewanella algae MARS 14 | diacylglycerol + lipid A (2-aminoethyl diphosphate) | - |
? | |
| additional information | lipid A substrate and product analysis by MALDI-TOF/MS. The lipidA preparations from transgenic Escherichia coli strains carrying EptA show additional ions due to the addition of phosphoethanolamine to the bis-phosphorylatedstructure and the hepta-acylated structure | Shewanella algae | ? | - |
? | |
| additional information | lipid A substrate and product analysis by MALDI-TOF/MS. The lipidA preparations from transgenic Escherichia coli strains carrying EptA show additional ions due to the addition of phosphoethanolamine to the bis-phosphorylatedstructure and the hepta-acylated structure | Shewanella algae MARS 14 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EptA | - |
Shewanella algae |
| ethanolamine phosphotransferase | - |
Shewanella algae |
| PmrC | - |
Shewanella algae |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the paradigm of resistance to antibiotic colistin mediated by ethanolamine phosphotransferase in Shewanella algae MARS 14. Resistance to colistin in Shewanella algae MARS 14 is associated with overexpression of enzyme EptA (27fold increase), which plays a crucial role in the arrangement of outer membrane lipopolysaccharide | Shewanella algae |
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