Cloned (Comment) | Organism |
---|---|
recombinant expression of His-tagged membrane-deletion mutant enzyme in Escherichia coli strain BL21(DE3)pLysS | Neisseria meningitidis |
Crystallization (Comment) | Organism |
---|---|
recombinant His-tagged membrane-deletion mutant enzyme, mixing of 0.001 ml of 5.2 mg/ml protein in 50 mM HEPES, pH 8.0, 50 mM sodium chloride, with 0.001 ml of crystallization solution containing 23-26% PEG 8000, 100 mM ammonium sulfate, 100 mM HEPES, pH 7.58.0, 15 mM n-dodecyl-N,N-dimethylamine-N-oxide, and 200 nl of microseeding solution, 20°C, 3-5 days, method optimization, X-ray diffraction structure determination and analysis at 1.7 A resolution | Neisseria meningitidis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a membrane-deletion mutant of the enzyme | Neisseria meningitidis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Neisseria meningitidis | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Neisseria meningitidis | Q7DDQ9 | gene lptA | - |
Neisseria meningitidis NMB | Q7DDQ9 | gene lptA | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged membrane-deletion mutant enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography and dialysis | Neisseria meningitidis |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme is composed of an N-terminal transmembrane domain and a C-terminal soluble domain. Tryptic peptide mapping and analysis by MALDI-TOF/TOF mass spectrometry | Neisseria meningitidis |
Synonyms | Comment | Organism |
---|---|---|
NmLptA | - |
Neisseria meningitidis |
phosphoethanolamine transferase A | - |
Neisseria meningitidis |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme phosphoethanolamine transferase A is involved in the addition of phosphoethanolamine moieties to lipid A | Neisseria meningitidis |