BRENDA - Enzyme Database
show all sequences of 2.7.8.43

Structural basis for the lipopolysaccharide export activity of the bacterial lipopolysaccharide transport system

Hicks, G.; Jia, Z.; Int. J. Mol. Sci. 19, E2680 (2018)
No PubMed abstract available

Data extracted from this reference:

Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
periplasm
LptA is the only component of the Lpt system that lacks a transmembrane component, as it spans the periplasmic space between the inner membrane and outer membrane complexes. LptA is bound to other Lpt components rather than floating freely in the periplasm
Escherichia coli
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-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
P0ADV1
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-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
specific LPS interactions with LptA and LptC
750867
Escherichia coli
?
-
-
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-
Subunits
Subunits
Commentary
Organism
oligomer
the enzyme LptA arranges in an end-to-end fibrous tetramer, which forms a continuous hydrophobic groove between the LptA monomers, crystal structure analysis. Mass spectral analysis confirmes that LptA forms 2-5-member oligomers in a concentration-dependent manner when purified in vitro and that the resultant complexes are stabilized by LPS. Analysis of subunit interactions
Escherichia coli
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
periplasm
LptA is the only component of the Lpt system that lacks a transmembrane component, as it spans the periplasmic space between the inner membrane and outer membrane complexes. LptA is bound to other Lpt components rather than floating freely in the periplasm
Escherichia coli
-
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
specific LPS interactions with LptA and LptC
750867
Escherichia coli
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
oligomer
the enzyme LptA arranges in an end-to-end fibrous tetramer, which forms a continuous hydrophobic groove between the LptA monomers, crystal structure analysis. Mass spectral analysis confirmes that LptA forms 2-5-member oligomers in a concentration-dependent manner when purified in vitro and that the resultant complexes are stabilized by LPS. Analysis of subunit interactions
Escherichia coli
General Information
General Information
Commentary
Organism
physiological function
the LPS (lipopolysaccharide) transport (Lpt) system, a coordinated seven-subunit protein complex that spans the cellular envelope. LPS transport is driven by an ATPase-dependent mechanism dubbed the PEZ model, whereby a continuous stream of LPS molecules is pushed from subunit to subunit, functional significance of LptA oligomerization and LptC. The membrane-bound LptB, F, G and C subunits are connected to the LptD/E heterodimer in the outer membrane by periplasmic LptA. The LptB2FG tetramer extracts LPS from the outer leaflet of the inner membrane and provides the energy to drive LPS transport through an ATPase-dependent mechanism. LptA provides a continuous LPS binding surface that conveys it to the outer membrane. Mechanism of the LPS (lipopolysaccharide) transport (Lpt) system, specific LPS interactions with LptA and LptC, LptC is the intermediate between the inner membrane complex and LptA, overview
Escherichia coli
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the LPS (lipopolysaccharide) transport (Lpt) system, a coordinated seven-subunit protein complex that spans the cellular envelope. LPS transport is driven by an ATPase-dependent mechanism dubbed the PEZ model, whereby a continuous stream of LPS molecules is pushed from subunit to subunit, functional significance of LptA oligomerization and LptC. The membrane-bound LptB, F, G and C subunits are connected to the LptD/E heterodimer in the outer membrane by periplasmic LptA. The LptB2FG tetramer extracts LPS from the outer leaflet of the inner membrane and provides the energy to drive LPS transport through an ATPase-dependent mechanism. LptA provides a continuous LPS binding surface that conveys it to the outer membrane. Mechanism of the LPS (lipopolysaccharide) transport (Lpt) system, specific LPS interactions with LptA and LptC, LptC is the intermediate between the inner membrane complex and LptA, overview
Escherichia coli
Other publictions for EC 2.7.8.43
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
750867
Hicks
-
Structural basis for the lipo ...
Escherichia coli
Int. J. Mol. Sci.
19
E2680
2018
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752134
Schultz
Lipopolysaccharide binding to ...
Escherichia coli
Protein Sci.
26
1517-1523
2017
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738476
Liu
A phosphoethanolamine transfer ...
Cronobacter sakazakii, Cronobacter sakazakii BAA894
J. Appl. Microbiol.
121
1444-1456
2016
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733635
Handing
The lipooligosaccharide-modify ...
Neisseria gonorrhoeae, Neisseria gonorrhoeae FA 1090
Cell. Microbiol.
17
910-921
2015
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735018
Trombley
Phosphoethanolamine transferas ...
Haemophilus ducreyi, Haemophilus ducreyi ATCC 700724
PLoS ONE
10
e0124373
2015
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739106
Nowicki
Extracellular zinc induces pho ...
Pseudomonas aeruginosa
Mol. Microbiol.
97
166-178
2015
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740532
Telke
Functional genomics to discove ...
Shewanella algae, Shewanella algae MARS 14
Int. J. Antimicrob. Agents
46
648-652
2015
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738368
Packiam
Phosphoethanolamine decoration ...
Neisseria gonorrhoeae, Neisseria gonorrhoeae FA 1090
Infect. Immun.
82
2170-2179
2014
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1
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11
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739495
Piek
The role of oxidoreductases in ...
Neisseria meningitidis, Neisseria meningitidis NMB
PLoS ONE
9
e106513
2014
1
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739786
Fage
Crystallographic study of the ...
Campylobacter jejuni, Campylobacter jejuni 81-176
Acta Crystallogr. Sect. D
70
2730-2739
2014
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752031
Sherman
Decoupling catalytic activity ...
Neisseria meningitidis serogroup B, Neisseria meningitidis serogroup B MC58
Proc. Natl. Acad. Sci. USA
111
4982-4987
2014
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733965
Lewis
Phosphoethanolamine residues o ...
Neisseria gonorrhoeae
Infect. Immun.
81
33-42
2013
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733966
Cullen
EptC of Campylobacter jejuni m ...
Campylobacter jejuni, Campylobacter jejuni 81-176
Infect. Immun.
81
430-4440
2013
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734476
Wanty
The structure of the neisseria ...
Neisseria meningitidis, Neisseria meningitidis NMB
J. Mol. Biol.
425
3389-3402
2013
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740134
Bontemps-Gallo
Biosynthesis of osmoregulated ...
Escherichia coli
BioMed Res. Int.
2013
371429
2013
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740516
Knirel
O-antigen structure of Shigell ...
Shigella flexneri
Glycobiology
23
475-485
2013
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733017
Anandan
Cloning, expression, purificat ...
Neisseria meningitidis, Neisseria meningitidis NMB
Acta Crystallogr. Sect. F
68
1494-1497
2012
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734190
Cullen
Trent, M.S.: Characterization ...
Campylobacter jejuni, Campylobacter jejuni 81-176
J. Biol. Chem.
287
326-3336
2012
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734198
Farizano
The PmrAB system-inducing cond ...
Salmonella enterica 14028s, Salmonella enterica
J. Biol. Chem.
287
38778-38789
2012
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733107
Beceiro
Phosphoethanolamine modificati ...
Acinetobacter baumannii
Antimicrob. Agents Chemother.
55
3370-3379
2011
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3
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723212
Herrera
Activation of PmrA inhibits Lp ...
Escherichia coli, Salmonella enterica, Salmonella enterica LT2
Mol. Microbiol.
76
1444-1460
2010
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735157
Cullen
A link between the assembly of ...
Campylobacter jejuni
Proc. Natl. Acad. Sci. USA
107
5160-5165
2010
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2
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733963
Lewis
Phosphoethanolamine substituti ...
Neisseria gonorrhoeae, Neisseria gonorrhoeae FA19
Infect. Immun.
77
1112-1120
2009
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1
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1
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6
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1
1
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733962
Takahashi
Modification of lipooligosacch ...
Neisseria meningitidis
Infect. Immun.
76
5777-5789
2008
-
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1
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1
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4
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1
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2
2
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734067
Tamayo
Identification of cptA, a PmrA ...
Salmonella enterica, Salmonella enterica LT2
J. Bacteriol.
187
3391-3399
2005
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1
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4
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5
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4
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4
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2
4
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734151
Tran
Resistance to the antimicrobia ...
Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium C5
J. Biol. Chem.
280
28186-28194
2005
-
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1
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3
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5
5
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734064
Lee
The PmrA-regulated pmrC gene m ...
Salmonella enterica
J. Bacteriol.
186
4124-4133
2004
1
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1
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3
3
-
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734148
Tran
Periplasmic cleavage and modif ...
Helicobacter pylori
J. Biol. Chem.
279
55780-55791
2004
-
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1
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3
-
4
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3
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3
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2
2
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734062
Cox
Phosphorylation of the lipid A ...
Neisseria meningitidis
J. Bacteriol.
185
3270-3277
2003
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-
1
-
1
-
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-
1
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3
-
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1
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1
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2
2
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734138
Zhou
Lipid A modifications in polym ...
Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium ATCC 14028
J. Biol. Chem.
276
43111-43121
2001
-
-
-
-
1
-
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-
-
2
-
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-
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2
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1
1
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734706
Gunn
PmrA-PmrB-regulated genes nece ...
Salmonella enterica 14028s, Salmonella enterica
Mol. Microbiol.
27
1171-1182
1998
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1
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