KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the two Km values for CDP-diacylglycerol, each estimated by nonlinear regression analysis of 18 kinetic points, are 0.02 and 0.03 mol% in the presence of 1 mM and 10 mM Mg2+ respectively, pH 9.0, 37°C | Saccharomyces cerevisiae | |
0.0024 | - |
CDP-1,2-dimyristoylglycerol | at low Co2+ concentration, pH pH 9.0, 37°C | Rattus norvegicus | |
0.0029 | - |
CDP-1,2-dimyristoylglycerol | at high Co2+ concentration, pH pH 9.0, 37°C | Rattus norvegicus | |
0.032 | - |
egg yolk phosphatidylglycerol | at high Co2+ concentration, pH pH 9.0, 37°C | Rattus norvegicus | |
0.0619 | - |
egg yolk phosphatidylglycerol | at low Co2+ concentration, pH pH 9.0, 37°C | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | most efficient cation for the activation. Co2+ activates cardiolipin formation by increasing the maximal enzyme activity from 2.9 pmol/min/mg protein at low Co2+ concentration to 4.1 pmol/min/mg protein at high Co2+ concentration | Rattus norvegicus | |
Mg2+ | 1.7 m required for half-maximal activity | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
a CDP-diacylglycerol + a phosphatidyl-glycerol | Rattus norvegicus | - |
a cardiolipin + CMP | - |
? | |
a CDP-diacylglycerol + a phosphatidyl-glycerol | Saccharomyces cerevisiae | - |
a cardiolipin + CMP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Rattus norvegicus |
partial | Saccharomyces cerevisiae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0000029 | - |
at low Co2+ concentration, pH 9.0, 37°C | Rattus norvegicus |
0.0000041 | - |
at high Co2+ concentration, pH 9.0, 37°C | Rattus norvegicus |
0.000513 | - |
pH 9.0, 37°C | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a CDP-diacylglycerol + a phosphatidyl-glycerol | - |
Rattus norvegicus | a cardiolipin + CMP | - |
? | |
a CDP-diacylglycerol + a phosphatidyl-glycerol | - |
Saccharomyces cerevisiae | a cardiolipin + CMP | - |
? | |
a CDP-diacylglycerol + a phosphatidylglycerol | - |
Saccharomyces cerevisiae | a cardiolipin + CMP | - |
? | |
CDP-1,2-dimyristoylglycerol + egg yolk phosphatidylglycerol | - |
Rattus norvegicus | a cardiolipin + CMP | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
37 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Rattus norvegicus |
9 | - |
assay at | Saccharomyces cerevisiae |