BRENDA - Enzyme Database
show all sequences of 2.7.8.39

A novel biosynthetic pathway of archaetidyl-myo-inositol via archaetidyl-myo-inositol phosphate from CDP-archaeol and D-glucose 6-phosphate in methanoarchaeon Methanothermobacter thermautotrophicus cells

Morii, H.; Kiyonari, S.; Ishino, Y.; Koga, Y.; J. Biol. Chem. 284, 30766-30774 (2009)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
Triton X-100
0.5%, stimulates
Methanothermobacter thermautotrophicus
Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Methanothermobacter thermautotrophicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Methanothermobacter thermautotrophicus
16020
-
membrane
-
Pyrococcus furiosus
16020
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
Mg2+ or Mn2+ required
Methanothermobacter thermautotrophicus
Mn2+
Mg2+ or Mn2+ required
Methanothermobacter thermautotrophicus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
20764
-
x * 20764, calculated from sequence
Methanothermobacter thermautotrophicus
20900
-
x * 20900, SDS-PAGE
Methanothermobacter thermautotrophicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
CDP-2,3-bis-O-(phytanyl)-sn-glycerol + 1L-myo-inositol 1-phosphate
Methanothermobacter thermautotrophicus
the enzyme is involved in biosynthesis of archaetidyl-myo-inositol
CMP + 1-archaetidyl-1D-myo-inositol 3-phosphate
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Methanothermobacter thermautotrophicus
O27726
-
-
Pyrococcus furiosus
-
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.02
-
pH 8.0, 60°C, crude enzyme
Methanothermobacter thermautotrophicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
CDP-2,3-bis-O-(phytanyl)-sn-glycerol + 1L-myo-inositol 1-phosphate
-
704559
Methanothermobacter thermautotrophicus
CMP + 1-archaetidyl-1D-myo-inositol 3-phosphate
-
-
-
?
CDP-2,3-bis-O-(phytanyl)-sn-glycerol + 1L-myo-inositol 1-phosphate
the enzyme is involved in biosynthesis of archaetidyl-myo-inositol
704559
Methanothermobacter thermautotrophicus
CMP + 1-archaetidyl-1D-myo-inositol 3-phosphate
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 20764, calculated from sequence; x * 20900, SDS-PAGE
Methanothermobacter thermautotrophicus
Synonyms
Synonyms
Commentary
Organism
AIP synthase
-
Methanothermobacter thermautotrophicus
MTH1691
-
Methanothermobacter thermautotrophicus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
assay at
Methanothermobacter thermautotrophicus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Methanothermobacter thermautotrophicus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
Triton X-100
0.5%, stimulates
Methanothermobacter thermautotrophicus
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Methanothermobacter thermautotrophicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Methanothermobacter thermautotrophicus
16020
-
membrane
-
Pyrococcus furiosus
16020
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
Mg2+ or Mn2+ required
Methanothermobacter thermautotrophicus
Mn2+
Mg2+ or Mn2+ required
Methanothermobacter thermautotrophicus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
20764
-
x * 20764, calculated from sequence
Methanothermobacter thermautotrophicus
20900
-
x * 20900, SDS-PAGE
Methanothermobacter thermautotrophicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
CDP-2,3-bis-O-(phytanyl)-sn-glycerol + 1L-myo-inositol 1-phosphate
Methanothermobacter thermautotrophicus
the enzyme is involved in biosynthesis of archaetidyl-myo-inositol
CMP + 1-archaetidyl-1D-myo-inositol 3-phosphate
-
-
?
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.02
-
pH 8.0, 60°C, crude enzyme
Methanothermobacter thermautotrophicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
CDP-2,3-bis-O-(phytanyl)-sn-glycerol + 1L-myo-inositol 1-phosphate
-
704559
Methanothermobacter thermautotrophicus
CMP + 1-archaetidyl-1D-myo-inositol 3-phosphate
-
-
-
?
CDP-2,3-bis-O-(phytanyl)-sn-glycerol + 1L-myo-inositol 1-phosphate
the enzyme is involved in biosynthesis of archaetidyl-myo-inositol
704559
Methanothermobacter thermautotrophicus
CMP + 1-archaetidyl-1D-myo-inositol 3-phosphate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 20764, calculated from sequence; x * 20900, SDS-PAGE
Methanothermobacter thermautotrophicus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
assay at
Methanothermobacter thermautotrophicus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Methanothermobacter thermautotrophicus
Other publictions for EC 2.7.8.39
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
726887
Morii
Ubiquitous distribution of pho ...
Aeropyrum pernix, Aeropyrum pernix DSM 11879, Saccharolobus solfataricus, Saccharolobus solfataricus P2
Biochem. Biophys. Res. Commun.
443
86-90
2013
-
-
2
-
-
-
-
-
-
-
-
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-
15
-
-
-
-
-
-
2
-
4
-
2
2
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
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-
-
-
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-
-
-
-
-
2
-
4
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
704559
Morii
A novel biosynthetic pathway o ...
Methanothermobacter thermautotrophicus, Pyrococcus furiosus
J. Biol. Chem.
284
30766-30774
2009
1
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1
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-
-
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2
2
2
1
-
6
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-
-
-
-
-
1
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2
1
2
1
-
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1
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-
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1
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1
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-
-
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-
2
2
2
1
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1
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2
1
1
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1
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