BRENDA - Enzyme Database
show all sequences of 2.7.8.38

CDP-2,3-di-O-geranylgeranyl-sn-glycerol:L-serine O-archaetidyltransferase (archaetidylserine synthase) in the methanogenic archaeon Methanothermobacter thermautotrophicus

Morii, H.; Koga, Y.; J. Bacteriol. 185, 1181-1189 (2003)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
Triton X-100
activity is dependent on TRiton X-100
Methanothermobacter thermautotrophicus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mn2+
stimulates, maximal activity in presence of 10 mM Mn2+. Mg2+ is much less effective
Methanothermobacter thermautotrophicus
additional information
the enzyme does not require the addition of K+. The enzyme activity is lowered to about 70% of its maximum at 0.6 M K+, which corresponds to the intracellular concentration found in Methanothermobacter thermautotrophicus
Methanothermobacter thermautotrophicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + L-serine
Methanothermobacter thermautotrophicus
the enzyme is involved in the biosynthesis of polar lipids in archaea
CMP + 2,3-bis-O-(geranylgeranyl)-sn-glycero-1-phospho-L-serine
i.e. unsaturated archaetidylserine
-
?
CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + L-serine
Methanothermobacter thermautotrophicus DSM 1053
the enzyme is involved in the biosynthesis of polar lipids in archaea
CMP + 2,3-bis-O-(geranylgeranyl)-sn-glycero-1-phospho-L-serine
i.e. unsaturated archaetidylserine
-
?
CDP-2,3-bis-O-(phytanyl)-sn-glycerol + L-serine
Bacillus subtilis
120% of the activity compared to CDP-1,2-diacylglycerol with fatty acids from lecithin
CMP + 2,3-bis-O-(phytanyl)-sn-glycero-1-phospho-L-serine
-
-
?
CDP-2,3-bis-O-(phytanyl)-sn-glycerol + L-serine
Methanothermobacter thermautotrophicus
86% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate
CMP + 2,3-bis-O-(phytanyl)-sn-glycero-1-phospho-L-serine
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus subtilis
-
-
-
Methanothermobacter thermautotrophicus
O27106
-
-
Methanothermobacter thermautotrophicus DSM 1053
O27106
-
-
Reaction
Reaction
Commentary
Organism
CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol + L-serine = CMP + 2,3-bis-(O-geranylgeranyl)-sn-glycero-1-phospho-L-serine
(1)
Methanothermobacter thermautotrophicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
CDP-1,2-bis-O-(geranylgeranyl)-sn-glycerol + L-serine
91% of the activity compared to CDP-1,2-diacylglycerol with fatty acids from lecithin
692823
Bacillus subtilis
CMP + 1,2-bis-O-(geranylgeranyl)-sn-glycero-3-phospho-L-serine
-
-
-
?
CDP-1,2-bis-O-(geranylgeranyl)-sn-glycerol + L-serine
149% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate
692823
Methanothermobacter thermautotrophicus
CMP + 1,2-bis-O-(geranylgeranyl)-sn-glycero-3-phosphoserine
-
-
-
?
CDP-1,2-bis-O-(geranylgeranyl)-sn-glycerol + L-serine
149% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate
692823
Methanothermobacter thermautotrophicus DSM 1053
CMP + 1,2-bis-O-(geranylgeranyl)-sn-glycero-3-phosphoserine
-
-
-
?
CDP-1,2-bis-O-(oleoyl)-sn-glycerol + L-serine
102% of the activity compared to CDP-1,2-diacylglycerol with fatty acids from lecithin
692823
Bacillus subtilis
CMP + 1,2-bis-O-(oleoyl)-sn-glycero-3-phospho-L-serine
-
-
-
?
CDP-1,2-bis-O-(oleoyl)-sn-glycerol + L-serine
64% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate
692823
Methanothermobacter thermautotrophicus
CMP + 1,2-bis-O-(oleoyl)-sn-glycero-3-phospho-L-serine
-
-
-
?
CDP-1,2-bis-O-(oleoyl)-sn-glycerol + L-serine
64% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate
692823
Methanothermobacter thermautotrophicus DSM 1053
CMP + 1,2-bis-O-(oleoyl)-sn-glycero-3-phospho-L-serine
-
-
-
?
CDP-1,2-diacylglycerol + L-serine
fatty acids from lecithin
692823
Bacillus subtilis
CMP + 1,2-diacyl-sn-glycero-3-phospho-L-serine
-
-
-
?
CDP-1,2-diacylglycerol + L-serine
fatty acids from lecithin. 199% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate
692823
Methanothermobacter thermautotrophicus
CMP + 1,2-diacyl-sn-glycero-3-phospho-L-serine
-
-
-
?
CDP-1,2-diacylglycerol + L-serine
fatty acids from lecithin. 199% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate
692823
Methanothermobacter thermautotrophicus DSM 1053
CMP + 1,2-diacyl-sn-glycero-3-phospho-L-serine
-
-
-
?
CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + L-serine
the enzyme is involved in the biosynthesis of polar lipids in archaea
692823
Methanothermobacter thermautotrophicus
CMP + 2,3-bis-O-(geranylgeranyl)-sn-glycero-1-phospho-L-serine
i.e. unsaturated archaetidylserine
-
-
?
CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + L-serine
i.e. CDP-unsaturated archaeol. 80% of the activity compared to CDP-1,2-diacylglycerol with fatty acids from lecithin
692823
Bacillus subtilis
CMP + 2,3-bis-O-(geranylgeranyl)-sn-glycero-1-phospho-L-serine
i.e. unsaturated archaetidylserine
-
-
?
CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + L-serine
i.e. CDP-unsaturated archaeol. The activity of D-serine with the enzyme is 30% of that observed for L-serine. Among various synthetic substrate analogs, both enantiomers of CDP-unsaturated archaeols with ether-linked geranylgeranyl chains and CDP-saturated archaeol with ether-linked phytanyl chains are similarly active toward the archaetidylserine synthase
692823
Methanothermobacter thermautotrophicus
CMP + 2,3-bis-O-(geranylgeranyl)-sn-glycero-1-phospho-L-serine
i.e. unsaturated archaetidylserine
-
-
?
CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + L-serine
the enzyme is involved in the biosynthesis of polar lipids in archaea
692823
Methanothermobacter thermautotrophicus DSM 1053
CMP + 2,3-bis-O-(geranylgeranyl)-sn-glycero-1-phospho-L-serine
i.e. unsaturated archaetidylserine
-
-
?
CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + L-serine
i.e. CDP-unsaturated archaeol. The activity of D-serine with the enzyme is 30% of that observed for L-serine. Among various synthetic substrate analogs, both enantiomers of CDP-unsaturated archaeols with ether-linked geranylgeranyl chains and CDP-saturated archaeol with ether-linked phytanyl chains are similarly active toward the archaetidylserine synthase
692823
Methanothermobacter thermautotrophicus DSM 1053
CMP + 2,3-bis-O-(geranylgeranyl)-sn-glycero-1-phospho-L-serine
i.e. unsaturated archaetidylserine
-
-
?
CDP-2,3-bis-O-(oleoyl)-sn-glycerol + L-serine
280% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate
692823
Methanothermobacter thermautotrophicus
CMP + 2,3-dioleoyl-sn-glycero-1-phospho-L-serine
-
-
-
?
CDP-2,3-bis-O-(phytanyl)-sn-glycerol + L-serine
120% of the activity compared to CDP-1,2-diacylglycerol with fatty acids from lecithin
692823
Bacillus subtilis
CMP + 2,3-bis-O-(phytanyl)-sn-glycero-1-phospho-L-serine
-
-
-
?
CDP-2,3-bis-O-(phytanyl)-sn-glycerol + L-serine
86% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate
692823
Methanothermobacter thermautotrophicus
CMP + 2,3-bis-O-(phytanyl)-sn-glycero-1-phospho-L-serine
-
-
-
?
CDP-2,3-dioleoyl-sn-glycerol + L-serine
143% of the activity compared to CDP-1,2-diacylglycerol with fatty acids from lecithin
692823
Bacillus subtilis
CMP + 2,3-bis-O-(oleoyl)-sn-glycero-1-phospho-L-serine
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
-
Methanothermobacter thermautotrophicus
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
50
70
50°C: about 50% of maximal activity, 70°C: about 65% of maximal activity
Methanothermobacter thermautotrophicus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
assay at
Methanothermobacter thermautotrophicus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
Triton X-100
activity is dependent on TRiton X-100
Methanothermobacter thermautotrophicus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mn2+
stimulates, maximal activity in presence of 10 mM Mn2+. Mg2+ is much less effective
Methanothermobacter thermautotrophicus
additional information
the enzyme does not require the addition of K+. The enzyme activity is lowered to about 70% of its maximum at 0.6 M K+, which corresponds to the intracellular concentration found in Methanothermobacter thermautotrophicus
Methanothermobacter thermautotrophicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + L-serine
Methanothermobacter thermautotrophicus
the enzyme is involved in the biosynthesis of polar lipids in archaea
CMP + 2,3-bis-O-(geranylgeranyl)-sn-glycero-1-phospho-L-serine
i.e. unsaturated archaetidylserine
-
?
CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + L-serine
Methanothermobacter thermautotrophicus DSM 1053
the enzyme is involved in the biosynthesis of polar lipids in archaea
CMP + 2,3-bis-O-(geranylgeranyl)-sn-glycero-1-phospho-L-serine
i.e. unsaturated archaetidylserine
-
?
CDP-2,3-bis-O-(phytanyl)-sn-glycerol + L-serine
Bacillus subtilis
120% of the activity compared to CDP-1,2-diacylglycerol with fatty acids from lecithin
CMP + 2,3-bis-O-(phytanyl)-sn-glycero-1-phospho-L-serine
-
-
?
CDP-2,3-bis-O-(phytanyl)-sn-glycerol + L-serine
Methanothermobacter thermautotrophicus
86% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate
CMP + 2,3-bis-O-(phytanyl)-sn-glycero-1-phospho-L-serine
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
CDP-1,2-bis-O-(geranylgeranyl)-sn-glycerol + L-serine
91% of the activity compared to CDP-1,2-diacylglycerol with fatty acids from lecithin
692823
Bacillus subtilis
CMP + 1,2-bis-O-(geranylgeranyl)-sn-glycero-3-phospho-L-serine
-
-
-
?
CDP-1,2-bis-O-(geranylgeranyl)-sn-glycerol + L-serine
149% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate
692823
Methanothermobacter thermautotrophicus
CMP + 1,2-bis-O-(geranylgeranyl)-sn-glycero-3-phosphoserine
-
-
-
?
CDP-1,2-bis-O-(geranylgeranyl)-sn-glycerol + L-serine
149% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate
692823
Methanothermobacter thermautotrophicus DSM 1053
CMP + 1,2-bis-O-(geranylgeranyl)-sn-glycero-3-phosphoserine
-
-
-
?
CDP-1,2-bis-O-(oleoyl)-sn-glycerol + L-serine
102% of the activity compared to CDP-1,2-diacylglycerol with fatty acids from lecithin
692823
Bacillus subtilis
CMP + 1,2-bis-O-(oleoyl)-sn-glycero-3-phospho-L-serine
-
-
-
?
CDP-1,2-bis-O-(oleoyl)-sn-glycerol + L-serine
64% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate
692823
Methanothermobacter thermautotrophicus
CMP + 1,2-bis-O-(oleoyl)-sn-glycero-3-phospho-L-serine
-
-
-
?
CDP-1,2-bis-O-(oleoyl)-sn-glycerol + L-serine
64% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate
692823
Methanothermobacter thermautotrophicus DSM 1053
CMP + 1,2-bis-O-(oleoyl)-sn-glycero-3-phospho-L-serine
-
-
-
?
CDP-1,2-diacylglycerol + L-serine
fatty acids from lecithin
692823
Bacillus subtilis
CMP + 1,2-diacyl-sn-glycero-3-phospho-L-serine
-
-
-
?
CDP-1,2-diacylglycerol + L-serine
fatty acids from lecithin. 199% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate
692823
Methanothermobacter thermautotrophicus
CMP + 1,2-diacyl-sn-glycero-3-phospho-L-serine
-
-
-
?
CDP-1,2-diacylglycerol + L-serine
fatty acids from lecithin. 199% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate
692823
Methanothermobacter thermautotrophicus DSM 1053
CMP + 1,2-diacyl-sn-glycero-3-phospho-L-serine
-
-
-
?
CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + L-serine
the enzyme is involved in the biosynthesis of polar lipids in archaea
692823
Methanothermobacter thermautotrophicus
CMP + 2,3-bis-O-(geranylgeranyl)-sn-glycero-1-phospho-L-serine
i.e. unsaturated archaetidylserine
-
-
?
CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + L-serine
i.e. CDP-unsaturated archaeol. 80% of the activity compared to CDP-1,2-diacylglycerol with fatty acids from lecithin
692823
Bacillus subtilis
CMP + 2,3-bis-O-(geranylgeranyl)-sn-glycero-1-phospho-L-serine
i.e. unsaturated archaetidylserine
-
-
?
CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + L-serine
i.e. CDP-unsaturated archaeol. The activity of D-serine with the enzyme is 30% of that observed for L-serine. Among various synthetic substrate analogs, both enantiomers of CDP-unsaturated archaeols with ether-linked geranylgeranyl chains and CDP-saturated archaeol with ether-linked phytanyl chains are similarly active toward the archaetidylserine synthase
692823
Methanothermobacter thermautotrophicus
CMP + 2,3-bis-O-(geranylgeranyl)-sn-glycero-1-phospho-L-serine
i.e. unsaturated archaetidylserine
-
-
?
CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + L-serine
the enzyme is involved in the biosynthesis of polar lipids in archaea
692823
Methanothermobacter thermautotrophicus DSM 1053
CMP + 2,3-bis-O-(geranylgeranyl)-sn-glycero-1-phospho-L-serine
i.e. unsaturated archaetidylserine
-
-
?
CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + L-serine
i.e. CDP-unsaturated archaeol. The activity of D-serine with the enzyme is 30% of that observed for L-serine. Among various synthetic substrate analogs, both enantiomers of CDP-unsaturated archaeols with ether-linked geranylgeranyl chains and CDP-saturated archaeol with ether-linked phytanyl chains are similarly active toward the archaetidylserine synthase
692823
Methanothermobacter thermautotrophicus DSM 1053
CMP + 2,3-bis-O-(geranylgeranyl)-sn-glycero-1-phospho-L-serine
i.e. unsaturated archaetidylserine
-
-
?
CDP-2,3-bis-O-(oleoyl)-sn-glycerol + L-serine
280% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate
692823
Methanothermobacter thermautotrophicus
CMP + 2,3-dioleoyl-sn-glycero-1-phospho-L-serine
-
-
-
?
CDP-2,3-bis-O-(phytanyl)-sn-glycerol + L-serine
120% of the activity compared to CDP-1,2-diacylglycerol with fatty acids from lecithin
692823
Bacillus subtilis
CMP + 2,3-bis-O-(phytanyl)-sn-glycero-1-phospho-L-serine
-
-
-
?
CDP-2,3-bis-O-(phytanyl)-sn-glycerol + L-serine
86% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate
692823
Methanothermobacter thermautotrophicus
CMP + 2,3-bis-O-(phytanyl)-sn-glycero-1-phospho-L-serine
-
-
-
?
CDP-2,3-dioleoyl-sn-glycerol + L-serine
143% of the activity compared to CDP-1,2-diacylglycerol with fatty acids from lecithin
692823
Bacillus subtilis
CMP + 2,3-bis-O-(oleoyl)-sn-glycero-1-phospho-L-serine
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
-
Methanothermobacter thermautotrophicus
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
50
70
50°C: about 50% of maximal activity, 70°C: about 65% of maximal activity
Methanothermobacter thermautotrophicus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
assay at
Methanothermobacter thermautotrophicus
Other publictions for EC 2.7.8.38
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
692823
Morii
CDP-2,3-di-O-geranylgeranyl-sn ...
Bacillus subtilis, Methanothermobacter thermautotrophicus, Methanothermobacter thermautotrophicus DSM 1053
J. Bacteriol.
185
1181-1189
2003
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