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Literature summary for 2.7.8.38 extracted from
- CDP-2,3-di-O-geranylgeranyl-sn-glycerol:L-serine O-archaetidyltransferase (archaetidylserine synthase) in the methanogenic archaeon Methanothermobacter thermautotrophicus (2003), J. Bacteriol., 185, 1181-1189.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | activity is dependent on TRiton X-100 | Methanothermobacter thermautotrophicus |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | stimulates, maximal activity in presence of 10 mM Mn2+. Mg2+ is much less effective | Methanothermobacter thermautotrophicus | |
| additional information | the enzyme does not require the addition of K+. The enzyme activity is lowered to about 70% of its maximum at 0.6 M K+, which corresponds to the intracellular concentration found in Methanothermobacter thermautotrophicus | Methanothermobacter thermautotrophicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + L-serine | Methanothermobacter thermautotrophicus | the enzyme is involved in the biosynthesis of polar lipids in archaea | CMP + 2,3-bis-O-(geranylgeranyl)-sn-glycero-1-phospho-L-serine | i.e. unsaturated archaetidylserine | ? | |
| CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + L-serine | Methanothermobacter thermautotrophicus DSM 1053 | the enzyme is involved in the biosynthesis of polar lipids in archaea | CMP + 2,3-bis-O-(geranylgeranyl)-sn-glycero-1-phospho-L-serine | i.e. unsaturated archaetidylserine | ? | |
| CDP-2,3-bis-O-(phytanyl)-sn-glycerol + L-serine | Bacillus subtilis | 120% of the activity compared to CDP-1,2-diacylglycerol with fatty acids from lecithin | CMP + 2,3-bis-O-(phytanyl)-sn-glycero-1-phospho-L-serine | - |
? | |
| CDP-2,3-bis-O-(phytanyl)-sn-glycerol + L-serine | Methanothermobacter thermautotrophicus | 86% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate | CMP + 2,3-bis-O-(phytanyl)-sn-glycero-1-phospho-L-serine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
| Methanothermobacter thermautotrophicus | O27106 | - |
- |
| Methanothermobacter thermautotrophicus DSM 1053 | O27106 | - |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol + L-serine = CMP + 2,3-bis-(O-geranylgeranyl)-sn-glycero-1-phospho-L-serine | (1) | Methanothermobacter thermautotrophicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CDP-1,2-bis-O-(geranylgeranyl)-sn-glycerol + L-serine | 91% of the activity compared to CDP-1,2-diacylglycerol with fatty acids from lecithin | Bacillus subtilis | CMP + 1,2-bis-O-(geranylgeranyl)-sn-glycero-3-phospho-L-serine | - |
? | |
| CDP-1,2-bis-O-(geranylgeranyl)-sn-glycerol + L-serine | 149% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate | Methanothermobacter thermautotrophicus | CMP + 1,2-bis-O-(geranylgeranyl)-sn-glycero-3-phosphoserine | - |
? | |
| CDP-1,2-bis-O-(geranylgeranyl)-sn-glycerol + L-serine | 149% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate | Methanothermobacter thermautotrophicus DSM 1053 | CMP + 1,2-bis-O-(geranylgeranyl)-sn-glycero-3-phosphoserine | - |
? | |
| CDP-1,2-bis-O-(oleoyl)-sn-glycerol + L-serine | 102% of the activity compared to CDP-1,2-diacylglycerol with fatty acids from lecithin | Bacillus subtilis | CMP + 1,2-bis-O-(oleoyl)-sn-glycero-3-phospho-L-serine | - |
? | |
| CDP-1,2-bis-O-(oleoyl)-sn-glycerol + L-serine | 64% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate | Methanothermobacter thermautotrophicus | CMP + 1,2-bis-O-(oleoyl)-sn-glycero-3-phospho-L-serine | - |
? | |
| CDP-1,2-bis-O-(oleoyl)-sn-glycerol + L-serine | 64% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate | Methanothermobacter thermautotrophicus DSM 1053 | CMP + 1,2-bis-O-(oleoyl)-sn-glycero-3-phospho-L-serine | - |
? | |
| CDP-1,2-diacylglycerol + L-serine | fatty acids from lecithin | Bacillus subtilis | CMP + 1,2-diacyl-sn-glycero-3-phospho-L-serine | - |
? | |
| CDP-1,2-diacylglycerol + L-serine | fatty acids from lecithin. 199% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate | Methanothermobacter thermautotrophicus | CMP + 1,2-diacyl-sn-glycero-3-phospho-L-serine | - |
? | |
| CDP-1,2-diacylglycerol + L-serine | fatty acids from lecithin. 199% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate | Methanothermobacter thermautotrophicus DSM 1053 | CMP + 1,2-diacyl-sn-glycero-3-phospho-L-serine | - |
? | |
| CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + L-serine | the enzyme is involved in the biosynthesis of polar lipids in archaea | Methanothermobacter thermautotrophicus | CMP + 2,3-bis-O-(geranylgeranyl)-sn-glycero-1-phospho-L-serine | i.e. unsaturated archaetidylserine | ? | |
| CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + L-serine | i.e. CDP-unsaturated archaeol. 80% of the activity compared to CDP-1,2-diacylglycerol with fatty acids from lecithin | Bacillus subtilis | CMP + 2,3-bis-O-(geranylgeranyl)-sn-glycero-1-phospho-L-serine | i.e. unsaturated archaetidylserine | ? | |
| CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + L-serine | i.e. CDP-unsaturated archaeol. The activity of D-serine with the enzyme is 30% of that observed for L-serine. Among various synthetic substrate analogs, both enantiomers of CDP-unsaturated archaeols with ether-linked geranylgeranyl chains and CDP-saturated archaeol with ether-linked phytanyl chains are similarly active toward the archaetidylserine synthase | Methanothermobacter thermautotrophicus | CMP + 2,3-bis-O-(geranylgeranyl)-sn-glycero-1-phospho-L-serine | i.e. unsaturated archaetidylserine | ? | |
| CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + L-serine | the enzyme is involved in the biosynthesis of polar lipids in archaea | Methanothermobacter thermautotrophicus DSM 1053 | CMP + 2,3-bis-O-(geranylgeranyl)-sn-glycero-1-phospho-L-serine | i.e. unsaturated archaetidylserine | ? | |
| CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + L-serine | i.e. CDP-unsaturated archaeol. The activity of D-serine with the enzyme is 30% of that observed for L-serine. Among various synthetic substrate analogs, both enantiomers of CDP-unsaturated archaeols with ether-linked geranylgeranyl chains and CDP-saturated archaeol with ether-linked phytanyl chains are similarly active toward the archaetidylserine synthase | Methanothermobacter thermautotrophicus DSM 1053 | CMP + 2,3-bis-O-(geranylgeranyl)-sn-glycero-1-phospho-L-serine | i.e. unsaturated archaetidylserine | ? | |
| CDP-2,3-bis-O-(oleoyl)-sn-glycerol + L-serine | 280% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate | Methanothermobacter thermautotrophicus | CMP + 2,3-dioleoyl-sn-glycero-1-phospho-L-serine | - |
? | |
| CDP-2,3-bis-O-(phytanyl)-sn-glycerol + L-serine | 120% of the activity compared to CDP-1,2-diacylglycerol with fatty acids from lecithin | Bacillus subtilis | CMP + 2,3-bis-O-(phytanyl)-sn-glycero-1-phospho-L-serine | - |
? | |
| CDP-2,3-bis-O-(phytanyl)-sn-glycerol + L-serine | 86% of the activity compared to CDP-2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate | Methanothermobacter thermautotrophicus | CMP + 2,3-bis-O-(phytanyl)-sn-glycero-1-phospho-L-serine | - |
? | |
| CDP-2,3-dioleoyl-sn-glycerol + L-serine | 143% of the activity compared to CDP-1,2-diacylglycerol with fatty acids from lecithin | Bacillus subtilis | CMP + 2,3-bis-O-(oleoyl)-sn-glycero-1-phospho-L-serine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol:L-serine O-archaetidyltransferase | - |
Bacillus subtilis |
| CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol:L-serine O-archaetidyltransferase | - |
Methanothermobacter thermautotrophicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Methanothermobacter thermautotrophicus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 70 | 50°C: about 50% of maximal activity, 70°C: about 65% of maximal activity | Methanothermobacter thermautotrophicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
assay at | Methanothermobacter thermautotrophicus |
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