Cloned (Comment) | Organism |
---|---|
GST fusion proteins of PhnI and PhnL expressed in Escherichia coli BL21(DE3), His-tagged PhnG and PhnH expressed in Escherichia coli BL21(DE3) | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
GST fusion proteins: GSTrap column, His-tagged proteins: HisTrap column | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Escherichia coli | adenine + D-ribose-5-triphosphate | - |
? | |
ATP + methylphosphonate | - |
Escherichia coli | adenine + alpha-D-ribose-1-methylphosphonate-5-triphosphate | - |
? | |
GTP + H2O | - |
Escherichia coli | guanine + D-ribose-5-triphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PhnI | in the presence of PhnG, PhnH and PhnL | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13 | - |
ATP | PhnI, nucleosidase activity, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
83 | - |
GTP | PhnI, nucleosidase activity, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
340 | - |
GTP | PhnI, PhnG, PhnH and PhnL, GST tags in situ removed, proteins begin to precipitate after removal of the GST tags, therefore kinetic constants are not definitive, pH not specified in the publication, temperature not specified in the publication | Escherichia coli | |
350 | - |
ATP | PhnI, PhnG, PhnH and PhnL, GST tags in situ removed, proteins begin to precipitate after removal of the GST tags, therefore kinetic constants are not definitive, pH not specified in the publication, temperature not specified in the publication | Escherichia coli |