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Literature summary for 2.7.8.33 extracted from
- Functional characterization and membrane topology of Escherichia coli WecA, a sugar-phosphate transferase initiating the biosynthesis of enterobacterial common antigen and O-antigen lipopolysaccharide (2007), J. Bacteriol., 189, 2618-2628.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D156E | no detectable transfer activity | Escherichia coli |
| D156N | no detectable transfer activity | Escherichia coli |
| D159E | transfer activity is drastically reduced, compared to wild-type enzyme | Escherichia coli |
| D159N | transfer activity is drastically reduced, compared to wild-type enzyme | Escherichia coli |
| D90E | slightly reduced velocities and small increases in the apparent Km for UDPGlcNAc. In membranes containing the D90E form of WecA, the apparent Km values for Mg2+ and Mn2+ increases 3-5fold compared to the apparent Km of the parental enzyme | Escherichia coli |
| D90N | slightly reduced velocities and small increases in the apparent Km for UDPGlcNAc. In membranes containing the D90N form of WecA, the apparent Km values for Mg2+ and Mn2+ increases 3-5fold compared to the apparent Km of the parental enzyme | Escherichia coli |
| D91E | membranes containing WecA-D91E exhibit a 6fold decrease in the apparent Km for UDP-GlcNAc compared to the apparent Km of the wild-type enzyme. In membranes containing the D91E form of WecA, the apparent Km values with Mg2+ decreases 3fold, compared to the apparent Km of the wild-type parental enzyme | Escherichia coli |
| D91N | in membranes containing the D91N form of WecA, the apparent Km values with Mg2+ increases 3fold, compared to the apparent Km of the wild-type parental enzyme | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00012 | - |
UDP-N-acetyl-D-glucosamine | pH 8.5, 37°C, in presence of Mg2+ | Escherichia coli |  |
| 0.00019 | - |
UDP-N-acetyl-D-glucosamine | pH 8.5, 37°C, in presence of Mn2+ | Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | integral membrane protein. the C terminus of WecA is exposed to the cytosol. Localizes to discrete regions in the bacterial plasma membrane | Escherichia coli | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | either Mg2+ or Mn2+ activates the enzyme. In vitro and in the presence of excess UDP-GlcNAc, the enzyme is nearly six times more effective with Mn2+ than with Mg2+. KM-values for wild-type and mutant enzymes | Escherichia coli | |
| Mn2+ | either Mg2+ or Mn2+ activates the enzyme. In vitro and in the presence of excess UDP-GlcNAc, the enzyme is nearly six times more effective with Mn2+ than with Mg2+. KM-values for wild-type and mutant enzymes | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
SDS-PAGE | Escherichia coli |
| 40957 | - |
x * 40957, calculated from sequence | Escherichia coli |
| 40960 | - |
calculated from sequence | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-D-glucosamine + ditrans,polycis-undecaprenyl phosphate | Escherichia coli | initiates the biosynthesis of enterobacterial common antigen and O-antigen lipopolysaccharide | UMP + N-acetyl-D-glucosaminyldiphospho-ditrans,polycis-undecaprenol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P0AC78 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-D-glucosamine + ditrans,polycis-undecaprenyl phosphate | initiates the biosynthesis of enterobacterial common antigen and O-antigen lipopolysaccharide | Escherichia coli | UMP + N-acetyl-D-glucosaminyldiphospho-ditrans,polycis-undecaprenol | - |
? | |
| UDP-N-acetyl-D-glucosamine + ditrans,polycis-undecaprenyl phosphate | Asp156 is required for catalysis | Escherichia coli | UMP + N-acetyl-D-glucosaminyldiphospho-ditrans,polycis-undecaprenol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 38000, SDS-PAGE | Escherichia coli |
| ? | x * 40957, calculated from sequence | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| WecA | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
assay at | Escherichia coli |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Escherichia coli | calculated from sequence | - |
10.01 |
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