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Literature summary for 2.7.8.32 extracted from

  • Reilly, M.C.; Levery, S.B.; Castle, S.A.; Klutts, J.S.; Doering, T.L.
    A novel xylosylphosphotransferase activity discovered in Cryptococcus neoformans (2009), J. Biol. Chem., 284, 36118-36127.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.8.32

Cloned(Commentary)

Cloned (Comment) Organism
complementation of the KN99alpha xpt1DELTA strain by episomal expression of XPT1 under the control of its native promoter (pXPT1) restores the manganese-dependent xylosyltransferase activity to wild-type levels Cryptococcus neoformans

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Cryptococcus neoformans

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane the Xpt1 sequence contains a single preducted transmembrane domain Cryptococcus neoformans 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 15% of the activity compared to Mn2+ as metal ion cofactor Cryptococcus neoformans
Mg2+ 15% of the activity compared to Mn2+ as metal ion cofactor Cryptococcus neoformans
Mn2+ preferred metal ion cofactor Cryptococcus neoformans
additional information no activity in presence of Ca2+, Cu2+, Ni2+, Zn2+ or Fe2+ Cryptococcus neoformans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
99527
-
 x * 99527, calculated from sequence Cryptococcus neoformans
99530
-
 calculated from sequence Cryptococcus neoformans

Organism

Organism UniProt Comment Textmining
Cryptococcus neoformans D0U690 var. grubii
-

Purification (Commentary)

Purification (Comment) Organism
affinity purification Cryptococcus neoformans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is specific for Man as an acceptor but is flexible as to the structural context of the mannosyl disaccharide Cryptococcus neoformans ?
-
 ?
UDP-xylose + 2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose high specificity for UDP-D-xylose. No activity with GDP-D-mannose, UDP-D-glucose, UDP-D-galactose, UDP-D-glucuronic acid or UDP-D-N-acetylglucosamine as donor Cryptococcus neoformans UMP + 2-O-(6-O-alpha-D-xylosylphosphono-alpha-D-mannopyranosyl)-alpha-D-mannopyranose
-
 ?
UDP-xylose + 3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose high specificity for UDP-D-xylose. No activity with GDP-D-mannose, UDP-D-glucose, UDP-D-galactose, UDP-D-glucuronic acid or UDP-D-N-acetylglucosamine as donor Cryptococcus neoformans UMP + 3-O-(6-O-alpha-D-xylosylphosphono-alpha-D-mannopyranosyl)-alpha-D-mannopyranose
-
 ?
UDP-xylose + 4-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose high specificity for UDP-D-xylose. No activity with GDP-D-mannose, UDP-D-glucose, UDP-D-galactose, UDP-D-glucuronic acid or UDP-D-N-acetylglucosamine as donor Cryptococcus neoformans UMP + 4-O-(6-O-alpha-D-xylosylphosphono-alpha-D-mannopyranosyl)-alpha-D-mannopyranose
-
 ?
UDP-xylose + 6-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose high specificity for UDP-D-xylose. No activity with GDP-D-mannose, UDP-D-glucose, UDP-D-galactose, UDP-D-glucuronic acid or UDP-D-N-acetylglucosamine as donor Cryptococcus neoformans UMP + 6-O-(6-O-alpha-D-xylosylphosphono-alpha-D-mannopyranosyl)-alpha-D-mannopyranose
-
 ?

Subunits

Subunits Comment Organism
? x * 99527, calculated from sequence Cryptococcus neoformans

Synonyms

Synonyms Comment Organism
XPT1
-
 Cryptococcus neoformans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
 assay at Cryptococcus neoformans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 assay at Cryptococcus neoformans

General Information

General Information Comment Organism
malfunction deletion of XPT1 yields a corresponding loss of the manganese-dependent xylosyltransferase product Cryptococcus neoformans