BRENDA - Enzyme Database
show all sequences of 2.7.8.32

A novel xylosylphosphotransferase activity discovered in Cryptococcus neoformans

Reilly, M.C.; Levery, S.B.; Castle, S.A.; Klutts, J.S.; Doering, T.L.; J. Biol. Chem. 284, 36118-36127 (2009)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
complementation of the KN99alpha xpt1DELTA strain by episomal expression of XPT1 under the control of its native promoter (pXPT1) restores the manganese-dependent xylosyltransferase activity to wild-type levels
Cryptococcus neoformans
Inhibitors
Inhibitors
Commentary
Organism
Structure
EDTA
-
Cryptococcus neoformans
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
the Xpt1 sequence contains a single preducted transmembrane domain
Cryptococcus neoformans
16020
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
15% of the activity compared to Mn2+ as metal ion cofactor
Cryptococcus neoformans
Mg2+
15% of the activity compared to Mn2+ as metal ion cofactor
Cryptococcus neoformans
Mn2+
preferred metal ion cofactor
Cryptococcus neoformans
additional information
no activity in presence of Ca2+, Cu2+, Ni2+, Zn2+ or Fe2+
Cryptococcus neoformans
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
99527
-
x * 99527, calculated from sequence
Cryptococcus neoformans
99530
-
calculated from sequence
Cryptococcus neoformans
Organism
Organism
UniProt
Commentary
Textmining
Cryptococcus neoformans
D0U690
var. grubii
-
Purification (Commentary)
Purification (Commentary)
Organism
affinity purification
Cryptococcus neoformans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
additional information
the enzyme is specific for Man as an acceptor but is flexible as to the structural context of the mannosyl disaccharide
704602
Cryptococcus neoformans
?
-
-
-
-
UDP-xylose + 2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose
high specificity for UDP-D-xylose. No activity with GDP-D-mannose, UDP-D-glucose, UDP-D-galactose, UDP-D-glucuronic acid or UDP-D-N-acetylglucosamine as donor
704602
Cryptococcus neoformans
UMP + 2-O-(6-O-alpha-D-xylosylphosphono-alpha-D-mannopyranosyl)-alpha-D-mannopyranose
-
-
-
?
UDP-xylose + 3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose
high specificity for UDP-D-xylose. No activity with GDP-D-mannose, UDP-D-glucose, UDP-D-galactose, UDP-D-glucuronic acid or UDP-D-N-acetylglucosamine as donor
704602
Cryptococcus neoformans
UMP + 3-O-(6-O-alpha-D-xylosylphosphono-alpha-D-mannopyranosyl)-alpha-D-mannopyranose
-
-
-
?
UDP-xylose + 4-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose
high specificity for UDP-D-xylose. No activity with GDP-D-mannose, UDP-D-glucose, UDP-D-galactose, UDP-D-glucuronic acid or UDP-D-N-acetylglucosamine as donor
704602
Cryptococcus neoformans
UMP + 4-O-(6-O-alpha-D-xylosylphosphono-alpha-D-mannopyranosyl)-alpha-D-mannopyranose
-
-
-
?
UDP-xylose + 6-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose
high specificity for UDP-D-xylose. No activity with GDP-D-mannose, UDP-D-glucose, UDP-D-galactose, UDP-D-glucuronic acid or UDP-D-N-acetylglucosamine as donor
704602
Cryptococcus neoformans
UMP + 6-O-(6-O-alpha-D-xylosylphosphono-alpha-D-mannopyranosyl)-alpha-D-mannopyranose
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 99527, calculated from sequence
Cryptococcus neoformans
Synonyms
Synonyms
Commentary
Organism
XPT1
-
Cryptococcus neoformans
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
20
-
assay at
Cryptococcus neoformans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
assay at
Cryptococcus neoformans
Cloned(Commentary) (protein specific)
Commentary
Organism
complementation of the KN99alpha xpt1DELTA strain by episomal expression of XPT1 under the control of its native promoter (pXPT1) restores the manganese-dependent xylosyltransferase activity to wild-type levels
Cryptococcus neoformans
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
EDTA
-
Cryptococcus neoformans
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
the Xpt1 sequence contains a single preducted transmembrane domain
Cryptococcus neoformans
16020
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
15% of the activity compared to Mn2+ as metal ion cofactor
Cryptococcus neoformans
Mg2+
15% of the activity compared to Mn2+ as metal ion cofactor
Cryptococcus neoformans
Mn2+
preferred metal ion cofactor
Cryptococcus neoformans
additional information
no activity in presence of Ca2+, Cu2+, Ni2+, Zn2+ or Fe2+
Cryptococcus neoformans
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
99527
-
x * 99527, calculated from sequence
Cryptococcus neoformans
99530
-
calculated from sequence
Cryptococcus neoformans
Purification (Commentary) (protein specific)
Commentary
Organism
affinity purification
Cryptococcus neoformans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
additional information
the enzyme is specific for Man as an acceptor but is flexible as to the structural context of the mannosyl disaccharide
704602
Cryptococcus neoformans
?
-
-
-
-
UDP-xylose + 2-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose
high specificity for UDP-D-xylose. No activity with GDP-D-mannose, UDP-D-glucose, UDP-D-galactose, UDP-D-glucuronic acid or UDP-D-N-acetylglucosamine as donor
704602
Cryptococcus neoformans
UMP + 2-O-(6-O-alpha-D-xylosylphosphono-alpha-D-mannopyranosyl)-alpha-D-mannopyranose
-
-
-
?
UDP-xylose + 3-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose
high specificity for UDP-D-xylose. No activity with GDP-D-mannose, UDP-D-glucose, UDP-D-galactose, UDP-D-glucuronic acid or UDP-D-N-acetylglucosamine as donor
704602
Cryptococcus neoformans
UMP + 3-O-(6-O-alpha-D-xylosylphosphono-alpha-D-mannopyranosyl)-alpha-D-mannopyranose
-
-
-
?
UDP-xylose + 4-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose
high specificity for UDP-D-xylose. No activity with GDP-D-mannose, UDP-D-glucose, UDP-D-galactose, UDP-D-glucuronic acid or UDP-D-N-acetylglucosamine as donor
704602
Cryptococcus neoformans
UMP + 4-O-(6-O-alpha-D-xylosylphosphono-alpha-D-mannopyranosyl)-alpha-D-mannopyranose
-
-
-
?
UDP-xylose + 6-O-alpha-D-mannopyranosyl-alpha-D-mannopyranose
high specificity for UDP-D-xylose. No activity with GDP-D-mannose, UDP-D-glucose, UDP-D-galactose, UDP-D-glucuronic acid or UDP-D-N-acetylglucosamine as donor
704602
Cryptococcus neoformans
UMP + 6-O-(6-O-alpha-D-xylosylphosphono-alpha-D-mannopyranosyl)-alpha-D-mannopyranose
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 99527, calculated from sequence
Cryptococcus neoformans
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
20
-
assay at
Cryptococcus neoformans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
-
assay at
Cryptococcus neoformans
General Information
General Information
Commentary
Organism
malfunction
deletion of XPT1 yields a corresponding loss of the manganese-dependent xylosyltransferase product
Cryptococcus neoformans
General Information (protein specific)
General Information
Commentary
Organism
malfunction
deletion of XPT1 yields a corresponding loss of the manganese-dependent xylosyltransferase product
Cryptococcus neoformans
Other publictions for EC 2.7.8.32
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
704602
Reilly
A novel xylosylphosphotransfer ...
Cryptococcus neoformans
J. Biol. Chem.
284
36118-36127
2009
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1
-
-
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1
-
1
4
2
-
-
1
-
-
1
-
-
-
-
-
5
1
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
4
2
-
-
-
-
1
-
-
-
-
5
1
1
-
-
-
1
-
-
-
-
1
1
-
-
-