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Literature summary for 2.7.8.29 extracted from

  • Kimura, A.K.; Kim, H.Y.
    Phosphatidylserine synthase 2: high efficiency for synthesizing phosphatidylserine containing docosahexaenoic acid (2013), J. Lipid Res., 54, 214-222.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.8.29

Cloned(Commentary)

Cloned (Comment) Organism
expressed in HEK-293 cells Mus musculus
gene PTDSS2, functional recombinant overexpression of FLAG-tagged PSS2 isozyme in HEK-293 and CHO-K1 cells Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Mus musculus 5783
-
membrane
-
 Mus musculus 16020
-
membrane integral membrane protein Mus musculus 16020
-
microsome
-
 Mus musculus
-
-
mitochondrial membrane
-
 Mus musculus 31966
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
-
 Mus musculus
50000
-
 x * 50000, calculated from amino acid sequence Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-1-phosphatidylcholine + L-serine Mus musculus isozyme PSS1 L-1-phosphatidylserine + choline
-
 r
L-1-phosphatidylethanolamine + L-serine Mus musculus isozyme PSS2 L-1-phosphatidylserine + ethanolamine
-
 r

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-
Mus musculus Q99LH2
-
-
Mus musculus Q9Z1X2
-
-

Purification (Commentary)

Purification (Comment) Organism
anti-FLAG M2 affinity gel chromatography Mus musculus
purification of functional recombinant FLAG-tagged PSS2 from HEK-293 and CHO-K1 cell microsomes by ultracentrifugation, immunoaffinity chromatography Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
 Mus musculus
-
additional information isozyme PSS1 is ubiquitously expressed Mus musculus
-
additional information isozyme PSS2 is expressed in a tissue-specific manner. It is highly expressed in tissues such as brain and testis, where docosahexaenoic acid (DHA, 22:6n-3) is also highly enriched Mus musculus
-
Purkinje neuron
-
 Mus musculus
-
Sertoli cell
-
 Mus musculus
-
testis
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-diacylphosphatidylethanolamine + L-serine the substrate is 6times better utilized than phosphatidylethanolamine plasmalogen Mus musculus ?
-
 ?
L-1-phosphatidylcholine + L-serine isozyme PSS1 Mus musculus L-1-phosphatidylserine + choline
-
 r
L-1-phosphatidylethanolamine + L-serine
-
 Mus musculus L-1-phosphatidylserine + ethanolamine
-
 ?
L-1-phosphatidylethanolamine + L-serine isozyme PSS2 Mus musculus L-1-phosphatidylserine + ethanolamine
-
 r
additional information the enzyme synthesizes docosahexaenoic acid-phosphatidylserine much more efficiently than phosphatidylserine containing either 18:1 or 20:4 at the sn-2 position. Although no sn-1 fatty acyl preference is noted, phosphatidylserine synthase 2 exhibits significant preference toward docosahexaenoic acid compared with 18:1 or 20:4 at the sn-2 position Mus musculus ?
-
 ?
additional information isozyme PSS1 shows a 2fold higher preference for sn-1 18:0 compared with 16:0 when the sn-2 position contains 18:1 Mus musculus ?
-
 ?
additional information the recombinant FLAG-tagged enzyme is also active with phosphatidylethanolamine plasmalogen, [C18(plasm),22:6-PS], which is six times less effective as a substrate than the corresponding ester-linked phosphatidylethanolamine species, the isozyme is active with oleic acid (OA; 18:1n-9), DHA (DHA; 22:6n-3), or arachidonic acid esters. Compounds analysis by mass spectroscopy. A carbonyl group next to the glycerol backbone in the substrate-binding pocket substantially increases the catalytic activity of PSS2. Isozyme PSS2 shows a 10fold preference for sn-1 18:0, when 22:6 is esterified at the sn-2 position. Synthesis of 18:0,22:6-PS is most efficient compared with 18:0,18:1-PS and with 18:0,20:4-PS. Substrate specificity and acyl chain preference of isozyme PS2, overview Mus musculus ?
-
 ?
phosphatidylethanolamine plasmalogen + L-serine
-
 Mus musculus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, calculated from amino acid sequence Mus musculus
? x * 50000, about, recombinant FLAG-tagged PSS2, SDS-PAGE Mus musculus

Synonyms

Synonyms Comment Organism
phosphatidylserine synthase 1
-
 Mus musculus
phosphatidylserine synthase 2
-
 Mus musculus
PS synthase 1
-
 Mus musculus
PS synthase 2
-
 Mus musculus
PSS1
-
 Mus musculus
PSS2
-
 Mus musculus
PTDSS2
-
 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Mus musculus

General Information

General Information Comment Organism
metabolism phosphatidylserine is synthesized in mammalian cells by two integral membrane proteins, PS synthase 1 and 2 (PSS1 and PSS2). These enzymes catalyze the formation of phosphatidylserine by an exchange reaction in which serine replaces the head group of the corresponding substrate phospholipids, phosphatidylcholine (PC) for isozyme PSS1 and phosphatidylethanolamine (PE) for isozyme PSS2 Mus musculus
physiological function the enzyme plays a key role in phosphatidylserine accumulation in brain and testis through high activity toward docosahexaenoic acid-containing substrates Mus musculus
physiological function isozyme PSS2 may play a key role in phosphatidylserine accumulation in brain and testis through high activity toward DHA-containing substrates that are abundant in these tissues Mus musculus