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Literature summary for 2.7.8.27 extracted from

  • Yeang, C.; Varshney, S.; Wang, R.; Zhang, Y.; Ye, D.; Jiang, X.C.
    The domain responsible for sphingomyelin synthase (SMS) activity (2008), Biochim. Biophys. Acta, 1781, 610-617.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.8.27

Application

Application Comment Organism
drug development SMS is a target for drug design Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
transient expression of Flag-tagged wild-type SMS2 and mutants in HeLa cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
H285A site-directed mutagenesis, inactive mutant with wild-type localization pattern, overview. The point mutation only influences SMS2 catalytic activity but not the enzyme topology Homo sapiens
H328A site-directed mutagenesis, inactive mutant with wild-type localization pattern, overview. The point mutation only influences SMS2 catalytic activity but not the enzyme topology Homo sapiens
H332A site-directed mutagenesis, inactive mutant with wild-type localization pattern, overview. The point mutation only influences SMS2 catalytic activity but not the enzyme topology Homo sapiens
S273A site-directed mutagenesis, inactive mutant with wild-type localization pattern, overview. The point mutation only influences SMS2 catalytic activity but not the enzyme topology Homo sapiens
S283A site-directed mutagenesis, inactive mutant with wild-type localization pattern, overview. The point mutation only influences SMS2 catalytic activity but not the enzyme topology Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus isozyme SMS1 Homo sapiens 5794
-
Golgi apparatus isozyme SMS2 Homo sapiens 5794
-
plasma membrane isozyme SMS2 Homo sapiens 5886
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens sphingomyelin synthase sits at the crossroads of sphingomyelin, ceramide, and diacylglycerol metabolism. It utilizes ceramide and phosphatidylcholine as substrates to produce sphingomyelin and diacylglycerol, thereby regulating lipid messengers which play a role in cell survival and apoptosis ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q86VZ5 SMS1; isozyme SMS1
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Homo sapiens Q8NHU3 SMS2; isozyme SMS2
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-alpha-phosphatidylcholine + NBD-C6-ceramide i.e. N-(6-[(7-nitro-benz-2-oxa-1,3-diazo-4-yl)amino]caproyl)-ceramide Homo sapiens ?
-
 ?
additional information sphingomyelin synthase sits at the crossroads of sphingomyelin, ceramide, and diacylglycerol metabolism. It utilizes ceramide and phosphatidylcholine as substrates to produce sphingomyelin and diacylglycerol, thereby regulating lipid messengers which play a role in cell survival and apoptosis Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
More SMS isozyme domain structure and active site comformation, tertiary structure model by homology modeling, overview Homo sapiens

Synonyms

Synonyms Comment Organism
SMS1
-
 Homo sapiens
SMS2
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens