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Literature summary for 2.7.8.26 extracted from

  • Zayas, C.L.; Claas, K.; Escalante-Semerena, J.C.
    The CbiB protein of Salmonella enterica is an integral membrane protein involved in the last step of the de novo corrin ring biosynthetic pathway (2007), J. Bacteriol., 189, 7697-7708.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.8.26

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha-ribazole-5'-phosphate + adenosylcobinamide-GDP Salmonella enterica second last step in AdoCbl biosynthetic pathway followed by dephosphorylation of adenosylcobalamin 5’-phosphate by alpha-ribazole-phosphate phosphatase (CobC) GMP + ?
-
 ?
alpha-ribazole-5'-phosphate + adenosylcobinamide-GDP Salmonella enterica LT2 second last step in AdoCbl biosynthetic pathway followed by dephosphorylation of adenosylcobalamin 5’-phosphate by alpha-ribazole-phosphate phosphatase (CobC) GMP + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Salmonella enterica
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 serovar Typhimurium TR6583, wild-type and cobC strain (no salvage of cobyric acid)
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Salmonella enterica LT2
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 serovar Typhimurium TR6583, wild-type and cobC strain (no salvage of cobyric acid)
-

Purification (Commentary)

Purification (Comment) Organism
solubilisation and extraction from Escherichia coli C41(DE3) membranes in presence of DHPC (15 mM) following overexpression with hexa-His-tagged from pET15b plasmid carrying CobS (pCobS5 (cobS+)) Salmonella enterica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-ribazole + adenosylcobinamide-GDP 1 h, 37°C, pH 9, in dim light, equimolar substrate concentration Salmonella enterica GMP + adenosylcobalamin reaction stop by KCN and incubation 10 min at 80°C, analysis by reverse-phase HPLC ?
alpha-ribazole + adenosylcobinamide-GDP 1 h, 37°C, pH 9, in dim light, equimolar substrate concentration Salmonella enterica LT2 GMP + adenosylcobalamin reaction stop by KCN and incubation 10 min at 80°C, analysis by reverse-phase HPLC ?
alpha-ribazole 5'-phosphate + adenosylcobinamide-GDP 1 h, 37°C, pH 9, in dim light, equimolar substrate concentration Salmonella enterica GMP + adenosylcobalamin 5'-phosphate reaction stop by KCN and incubation 10 min at 80°C, analysis by reverse-phase HPLC ?
alpha-ribazole 5'-phosphate + adenosylcobinamide-GDP 1 h, 37°C, pH 9, in dim light, equimolar substrate concentration Salmonella enterica LT2 GMP + adenosylcobalamin 5'-phosphate reaction stop by KCN and incubation 10 min at 80°C, analysis by reverse-phase HPLC ?
alpha-ribazole-5'-phosphate + adenosylcobinamide-GDP second last step in AdoCbl biosynthetic pathway followed by dephosphorylation of adenosylcobalamin 5’-phosphate by alpha-ribazole-phosphate phosphatase (CobC) Salmonella enterica GMP + ?
-
 ?
alpha-ribazole-5'-phosphate + adenosylcobinamide-GDP second last step in AdoCbl biosynthetic pathway followed by dephosphorylation of adenosylcobalamin 5’-phosphate by alpha-ribazole-phosphate phosphatase (CobC) Salmonella enterica LT2 GMP + ?
-
 ?
additional information about 100fold more efficient with alpha-ribazole-5’-phosphate as substrate than using alpha-ribazole, in vivo Salmonella enterica ?
-
 ?
additional information about 100fold more efficient with alpha-ribazole-5’-phosphate as substrate than using alpha-ribazole, in vivo Salmonella enterica LT2 ?
-
 ?

Synonyms

Synonyms Comment Organism
adenosylcobalamin synthase
-
 Salmonella enterica
AdoCbl-5’-P synthase
-
 Salmonella enterica
CobS
-
 Salmonella enterica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.67
-
 alpha-ribazole
-
 Salmonella enterica
83.3
-
 (4-chloro-3,5-dimethyl-1H-pyrazol-1-yl) (naphthalen-2-yl)methanone
-
 Salmonella enterica