Inhibitors | Comment | Organism | Structure |
---|---|---|---|
arbutin | at 5 mM, competitive to membrane-derived oligosaccharides | Escherichia coli | |
beta-gentiobiose | at 1-5 mM, competitive to membrane-derived oligosaccharides | Escherichia coli | |
EDTA | at 0.1 mM, for 1 h at 0ºC, activity is reduced to 40% of the control value. The activity is fully restored by addition of 0.5 mM MnCl2 | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
inner membrane | the active site is localized on the periplasmic face of the inner membrane | Escherichia coli | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | divalent cation required, Ca2+ is less effective than Mn2+ | Escherichia coli | |
Mg2+ | divalent cation required, less effective than Mn2+. At the optimal concentration, 0.5 mM, the activity is 67% of that observed with the same concentration of MnCl2 | Escherichia coli | |
Mn2+ | divalent cation required, Mn2+ is the most effective | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidylglycerol + membrane-derived oligosaccharide D-glucose | Escherichia coli | - |
sn-1,2-diacylglycerol + beta-D-glucoside-6-phosphoglycerol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
DF214, LIN205 and AB1133 strains | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidylglycerol + (R)-alpha[(6-O-beta-D-glucopyranosyl-beta-D-glucopyranosyl)oxyl]benzeneacetonitrile | i.e. amygdalin | Escherichia coli | sn-1,2-diacylglycerol + (R)-alpha[(6-O-beta-D-glucopyranosyl-beta-D-glucopyranosyl)oxyl]benzeneacetonitrile-6-phosphoglycerol | - |
? | |
phosphatidylglycerol + beta-gentiobiose | - |
Escherichia coli | sn-1,2-diacylglycerol + beta-gentiobiose-6-phosphoglycerol | - |
? | |
phosphatidylglycerol + membrane-derived oligosaccharide D-glucose | - |
Escherichia coli | sn-1,2-diacylglycerol + beta-D-glucoside-6-phosphoglycerol | - |
? | |
phosphatidylglycerol + membrane-derived oligosaccharide D-glucose | 1,2-beta- and 1,6-beta-linked glucose residues in membrane polysaccharides and in synthetic glucosides can act as acceptors | Escherichia coli | sn-1,2-diacylglycerol + beta-D-glucoside-6-phosphoglycerol | - |
? | |
phosphatidylglycerol + membrane-derived oligosaccharide D-glucose | the enzyme is specific for beta-glucosides | Escherichia coli | sn-1,2-diacylglycerol + beta-D-glucoside-6-phosphoglycerol | - |
? | |
phosphatidylglycerol + o-nitrophenyl-beta-D-glucoside | - |
Escherichia coli | sn-1,2-diacylglycerol + o-nitrophenyl-beta-D-glucoside-6-phosphoglycerol | - |
? | |
phosphatidylglycerol + octyl-beta-D-glucoside | - |
Escherichia coli | sn-1,2-diacylglycerol + octyl-beta-D-glucoside-6-phosphoglycerol | - |
? | |
phosphatidylglycerol + p-hydroxyphenyl-beta-D-glucoside | i.e. arbutin | Escherichia coli | sn-1,2-diacylglycerol + p-hydroxyphenyl-beta-D-glucoside-6-phosphoglycerol | - |
? | |
phosphatidylglycerol + p-nitrophenyl-beta-D-glucoside | - |
Escherichia coli | sn-1,2-diacylglycerol + p-nitrophenyl-beta-D-glucoside-6-phosphoglycerol | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.9 | - |
- |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.8 | 9.7 | 50% of optimal activity at pH 7.8 and pH 9.7 | Escherichia coli |