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Literature summary for 2.7.8.20 extracted from

  • Jackson, B.J.; Kennedy, E.P.
    The biosynthesis of membrane-derived oligosaccharides. A membrane-bound phosphoglycerol transferase (1983), J. Biol. Chem., 258, 2394-2398.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
arbutin at 5 mM, competitive to membrane-derived oligosaccharides Escherichia coli
beta-gentiobiose at 1-5 mM, competitive to membrane-derived oligosaccharides Escherichia coli
EDTA at 0.1 mM, for 1 h at 0ºC, activity is reduced to 40% of the control value. The activity is fully restored by addition of 0.5 mM MnCl2 Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
inner membrane the active site is localized on the periplasmic face of the inner membrane Escherichia coli
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ divalent cation required, Ca2+ is less effective than Mn2+ Escherichia coli
Mg2+ divalent cation required, less effective than Mn2+. At the optimal concentration, 0.5 mM, the activity is 67% of that observed with the same concentration of MnCl2 Escherichia coli
Mn2+ divalent cation required, Mn2+ is the most effective Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phosphatidylglycerol + membrane-derived oligosaccharide D-glucose Escherichia coli
-
sn-1,2-diacylglycerol + beta-D-glucoside-6-phosphoglycerol
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
DF214, LIN205 and AB1133 strains
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phosphatidylglycerol + (R)-alpha[(6-O-beta-D-glucopyranosyl-beta-D-glucopyranosyl)oxyl]benzeneacetonitrile i.e. amygdalin Escherichia coli sn-1,2-diacylglycerol + (R)-alpha[(6-O-beta-D-glucopyranosyl-beta-D-glucopyranosyl)oxyl]benzeneacetonitrile-6-phosphoglycerol
-
?
phosphatidylglycerol + beta-gentiobiose
-
Escherichia coli sn-1,2-diacylglycerol + beta-gentiobiose-6-phosphoglycerol
-
?
phosphatidylglycerol + membrane-derived oligosaccharide D-glucose
-
Escherichia coli sn-1,2-diacylglycerol + beta-D-glucoside-6-phosphoglycerol
-
?
phosphatidylglycerol + membrane-derived oligosaccharide D-glucose 1,2-beta- and 1,6-beta-linked glucose residues in membrane polysaccharides and in synthetic glucosides can act as acceptors Escherichia coli sn-1,2-diacylglycerol + beta-D-glucoside-6-phosphoglycerol
-
?
phosphatidylglycerol + membrane-derived oligosaccharide D-glucose the enzyme is specific for beta-glucosides Escherichia coli sn-1,2-diacylglycerol + beta-D-glucoside-6-phosphoglycerol
-
?
phosphatidylglycerol + o-nitrophenyl-beta-D-glucoside
-
Escherichia coli sn-1,2-diacylglycerol + o-nitrophenyl-beta-D-glucoside-6-phosphoglycerol
-
?
phosphatidylglycerol + octyl-beta-D-glucoside
-
Escherichia coli sn-1,2-diacylglycerol + octyl-beta-D-glucoside-6-phosphoglycerol
-
?
phosphatidylglycerol + p-hydroxyphenyl-beta-D-glucoside i.e. arbutin Escherichia coli sn-1,2-diacylglycerol + p-hydroxyphenyl-beta-D-glucoside-6-phosphoglycerol
-
?
phosphatidylglycerol + p-nitrophenyl-beta-D-glucoside
-
Escherichia coli sn-1,2-diacylglycerol + p-nitrophenyl-beta-D-glucoside-6-phosphoglycerol
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.9
-
-
Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
7.8 9.7 50% of optimal activity at pH 7.8 and pH 9.7 Escherichia coli