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Literature summary for 2.7.8.2 extracted from
- Phosphatidylcholine and the CDP-choline cycle (2013), Biochim. Biophys. Acta, 1831, 523-532.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | since CPT expression is associated with elaboration of new endoplasmic reticulum membrane, it may be useful as a marker protein for endoplasmic reticulum expansion | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis | Glycine max |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | detergent micelles that are used to deliver the DAG substrate for the assay can inhibit the reaction | Glycine max | |
| additional information | detergent micelles that are used to deliver the DAG substrate for the assay can inhibit the reaction | Homo sapiens | |
| additional information | detergent micelles that are used to deliver the DAG substrate for the assay can inhibit the reaction | Saccharomyces cerevisiae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | - |
Homo sapiens | 5783 | - |
| endoplasmic reticulum membrane | - |
Homo sapiens | 5789 | - |
| endoplasmic reticulum membrane | - |
Glycine max | 5789 | - |
| endoplasmic reticulum membrane | - |
Saccharomyces cerevisiae | 5789 | - |
| Golgi apparatus | - |
Glycine max | 5794 | - |
| Golgi apparatus | - |
Saccharomyces cerevisiae | 5794 | - |
| Golgi apparatus | mainly | Homo sapiens | 5794 | - |
| membrane | an integral membrane protein | Homo sapiens | 16020 | - |
| membrane | an integral membrane protein | Glycine max | 16020 | - |
| membrane | an integral membrane protein | Saccharomyces cerevisiae | 16020 | - |
| microsome | - |
Homo sapiens | - |
- |
| microsome | - |
Glycine max | - |
- |
| microsome | - |
Saccharomyces cerevisiae | - |
- |
| mitochondrial-associated membrane | co-purifies with crude mitochondria during subcellular fractionation of organelles | Homo sapiens | - |
- |
| mitochondrial-associated membrane | co-purifies with crude mitochondria during subcellular fractionation of organelles | Glycine max | - |
- |
| mitochondrial-associated membrane | co-purifies with crude mitochondria during subcellular fractionation of organelles | Saccharomyces cerevisiae | - |
- |
| additional information | CPT/CEPT enzymes reside in multiple subcellular membranes that can move among organelles | Homo sapiens | - |
- |
| additional information | the enzyme localization is not altered upon treatment of cells with brefeldin A | Homo sapiens | - |
- |
| nuclear membrane | - |
Homo sapiens | 31965 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CDP-choline + 1,2-diacyl-sn-glycerol | Homo sapiens | - |
CMP + a phosphatidylcholine | - |
r |  |
| CDP-choline + 1,2-diacyl-sn-glycerol | Glycine max | - |
CMP + a phosphatidylcholine | - |
r | |
| CDP-choline + 1,2-diacyl-sn-glycerol | Saccharomyces cerevisiae | - |
CMP + a phosphatidylcholine | - |
r | |
| CDP-ethanolamine + 1,2-diacyl-sn-glycerol | Homo sapiens | - |
CMP + a phosphatidylethanolamine | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Glycine max | I1KH05 | - |
- |
| Homo sapiens | Q8WUD6 | - |
- |
| Homo sapiens | Q9Y6K0 | - |
- |
| Saccharomyces cerevisiae | P17898 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| B-lymphocyte | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CDP-choline + 1,2-diacyl-sn-glycerol | - |
Homo sapiens | CMP + a phosphatidylcholine | - |
r | |
| CDP-choline + 1,2-diacyl-sn-glycerol | - |
Glycine max | CMP + a phosphatidylcholine | - |
r | |
| CDP-choline + 1,2-diacyl-sn-glycerol | - |
Saccharomyces cerevisiae | CMP + a phosphatidylcholine | - |
r | |
| CDP-ethanolamine + 1,2-diacyl-sn-glycerol | - |
Homo sapiens | CMP + a phosphatidylethanolamine | - |
r | |
| additional information | the enzyme has dual specificity for both CDP-choline and CDP-ethanolamine, EC 2.7.8.1 | Homo sapiens | ? | - |
? | |
| additional information | the enzyme is specific for CDP-choline, no activity with CDP-ethanolamine. The reaction occurs at the hydrophobic-hydrophilic interface of membranes which impacts kinetic behavior | Homo sapiens | ? | - |
? | |
| additional information | the enzyme is specific for CDP-choline, no activity with CDP-ethanolamine. The reaction occurs at the hydrophobic-hydrophilic interface of membranes which impacts kinetic behavior | Glycine max | ? | - |
? | |
| additional information | the enzyme is specific for CDP-choline, no activity with CDP-ethanolamine. The reaction occurs at the hydrophobic-hydrophilic interface of membranes which impacts kinetic behavior | Saccharomyces cerevisiae | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CEPT | - |
Homo sapiens |
| choline phosphotransferase | - |
Homo sapiens |
| choline phosphotransferase | - |
Glycine max |
| choline phosphotransferase | - |
Saccharomyces cerevisiae |
| CPT | - |
Homo sapiens |
| CPT | - |
Glycine max |
| CPT/CEPT | - |
Homo sapiens |
| CPT1 | - |
Saccharomyces cerevisiae |
| More | cf. EC 2.7.8.1 | Homo sapiens |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Homo sapiens | expression of the gene encoding CPT, but not CEPT, EC 2.7.8.1, increases during lipopolysaccahride-induced endoplasmic reticulum biogenesis in B-lymphocytes | additional information |
| Homo sapiens | expression of the gene encoding CPT, but not CEPT, EC 2.7.8.1, increases during lipopolysaccahride-induced endoplasmic reticulum biogenesis in B-lymphocytes | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | inhibition of the CPT activity reduces phospholipid synthesis and results in accumulation of CPT substrates. Reduction of phosphatidylcholine, e.g. by bacterial infection, results in apoptosis | Homo sapiens |
| malfunction | reduction of phosphatidylcholine, e.g. by bacterial infection, results in apoptosis | Homo sapiens |
| metabolism | CPT catalyzes the final step in the synthesis of phosphatidylcholine via the Kennedy pathway by the transfer of phosphocholine from CDP-choline to 1,2-diacyl-sn-glycerol. The CDP-choline and CDP-ethanolamine pathways are separate routes of phospholipid biosynthesis in mammalian cells, although the CEPT is probably a participant in both pathways | Glycine max |
| metabolism | CPT catalyzes the final step in the synthesis of phosphatidylcholine via the Kennedy pathway by the transfer of phosphocholine from CDP-choline to 1,2-diacyl-sn-glycerol. The CDP-choline and CDP-ethanolamine pathways are separate routes of phospholipid biosynthesis in mammalian cells, although the CEPT is probably a participant in both pathways | Saccharomyces cerevisiae |
| metabolism | CPT catalyzes the final step in the synthesis of phosphatidylcholine via the Kennedy pathway by the transfer of phosphocholine from CDP-choline to 1,2-diacyl-sn-glycerol. The CDP-choline and CDP-ethanolamine pathways are separate routes of phospholipid biosynthesis in mammalian cells, although the CEPT is probably a participant in both pathways. Molecular regulation of the CDP-choline pathway, DNA synthesis and phosphatidylcholine synthesis are not linked, overview. The CDP-choline pathway of phosphatidylcholine synthesis is essential in proliferating cells. The phosphatidylcholine pool is dynamic and the amount of PtdCho represents a balance between synthesis and degradation. phosphatidylcholine and other phospholipids are synthesized in the G2/M phase, rather than S phase. Physiological role of the CDP-choline pathway, overview | Homo sapiens |
| metabolism | the CDP-choline and CDP-ethanolamine pathways are separate routes of phospholipid biosynthesis in mammalian cells, although the CEPT is probably a participant in both pathways. The CPT activity responsible for the synthesis of platelet-activating factor (PAF) is different from those responsible for PtdCho synthesis on the basis of the enzyme's sensitivity to dithiothreitol. Molecular regulation of the CDP-choline pathway, overview. The CDP-choline pathway of phosphatidylcholine synthesis is essential in proliferating cells. The phosphatidylcholine pool is dynamic and the amount of PtdCho represents a balance between synthesis and degradation. Physiological role of the CDP-choline pathway, overview | Homo sapiens |
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