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Literature summary for 2.7.8.2 extracted from

  • Chen, Y.; Chi, H.Y.; Meesapyodsuk, D.; Qiu, X.
    Phytophthora infestans cholinephosphotransferase with substrate specificity for very-long-chain polyunsaturated fatty acids (2013), Appl. Environ. Microbiol., 79, 1573-1579.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.8.2

Cloned(Commentary)

Cloned (Comment) Organism
expressed in yeast Phytophthora infestans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02098
-
 CDP-choline pH 7.5, 25°C Phytophthora infestans

Organism

Organism UniProt Comment Textmining
Phytophthora infestans
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00323
-
 substrate: CDP-choline, pH 7.5, 25°C Phytophthora infestans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CDP-choline + 1,2-dioleoyl-sn-glycerol the most preferred diacylglycerol substrate is 18:0/20:4 diacylglycerol, followed by 18:0/22:6 diacylglycerol. The specific activities for 18:0/20:4 diacylglycerol and 18:0/22:6 diacylglycerol are 26 and 14fold higher than those of other diacylglycerol species Phytophthora infestans ?
-
 ?

Synonyms

Synonyms Comment Organism
PiCPT1
-
 Phytophthora infestans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
23
-
 assay at Phytophthora infestans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Phytophthora infestans

Expression

Organism Comment Expression
Phytophthora infestans expression of PiCPT1 is upregulated in Phytophthora infestans organisms fed with exogenous very long-chain polyunsaturated fatty acids up