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Literature summary for 2.7.8.2 extracted from

  • Ishida, N.; Irikura, D.; Matsuda, K.; Sato, S.; Sone, T.; Tanaka, M.; Asano, K.
    Molecular cloning and expression of a novel cholinephosphotransferase involved in glycoglycerophospholipid biosynthesis of Mycoplasma fermentans (2009), Curr. Microbiol., 58, 535-540.
    View publication on PubMed
Search for more literature for 2.7.8.2

Cloned(Commentary)

Cloned (Comment) Organism
into the vectors pCRBluntII, pENTR/D-TOPO and pDEST14, finally the pDEST14 construct is used for the expression of the protein in Escherichia coli BL21-AI cells Mycoplasmopsis fermentans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 determined by SDS-PAGE Mycoplasmopsis fermentans
31100
-
 theoretical Mycoplasmopsis fermentans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-alpha-D-glucopyranosyl-1,2-di-palmitoylglycerol + CDP-choline Mycoplasmopsis fermentans
-
 CMP + 3-(6-O-cholinephosphoryl-alpha-D-glucopyranosyl)-1,2-dipalmitoylglycerol
-
 ?
3-alpha-D-glucopyranosyl-1,2-di-palmitoylglycerol + CDP-choline Mycoplasmopsis fermentans PG18
-
 CMP + 3-(6-O-cholinephosphoryl-alpha-D-glucopyranosyl)-1,2-dipalmitoylglycerol
-
 ?

Organism

Organism UniProt Comment Textmining
Mycoplasmopsis fermentans C4TGF1
-
-
Mycoplasmopsis fermentans PG18 C4TGF1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-alpha-D-glucopyranosyl-1,2-di-palmitoylglycerol + CDP-choline
-
 Mycoplasmopsis fermentans CMP + 3-(6-O-cholinephosphoryl-alpha-D-glucopyranosyl)-1,2-dipalmitoylglycerol
-
 ?
3-alpha-D-glucopyranosyl-1,2-di-palmitoylglycerol + CDP-choline
-
 Mycoplasmopsis fermentans PG18 CMP + 3-(6-O-cholinephosphoryl-alpha-D-glucopyranosyl)-1,2-dipalmitoylglycerol
-
 ?

Synonyms

Synonyms Comment Organism
cholinephosphotransferase
-
 Mycoplasmopsis fermentans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 activity assay Mycoplasmopsis fermentans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 activity assay Mycoplasmopsis fermentans

General Information

General Information Comment Organism
physiological function cholinephosphotransferase is involved in glycoglycerophospholipid biosynthesis Mycoplasmopsis fermentans