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Literature summary for 2.7.8.2 extracted from
- Phospholipid synthesis in isolated fat cells. Studies of microsomal diacylglycerol cholinephosphotransferase and diacylglycerol ethanolaminephosphotransferase activities (1977), J. Biol. Chem., 252, 3050-3056.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EGTA | 0.5 mM, stimulates | Rattus norvegicus |  |
| ethylene glycol bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid | stimulates | Rattus norvegicus | |
| Phospholipase C | 23°C, 10 min, slight stimulation | Rattus norvegicus |
General Stability
| General Stability | Organism |
|---|---|
| freezing and thawing the microsomal preparation 5times destroys activity by 50% | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-butanol | - |
Rattus norvegicus | |
| acetone | - |
Rattus norvegicus | |
| Ca2+ | - |
Rattus norvegicus | |
| CDP | - |
Rattus norvegicus | |
| CDPethanolamine | - |
Rattus norvegicus | |
| dithiothreitol | - |
Rattus norvegicus | |
| methanol | - |
Rattus norvegicus | |
| palmitoyl-CoA | - |
Rattus norvegicus | |
| Triton X-100 | WR 1339; X-100 | Rattus norvegicus | |
| Trypsin | - |
Rattus norvegicus | |
| Tween-20 | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Rattus norvegicus | |
| 0.0057 | - |
didodecanoylglycerol | 23°C, pH 8.5 | Rattus norvegicus | |
| 0.0141 | - |
didecanoylglycerol | 23°C, pH 8.5 | Rattus norvegicus | |
| 0.0239 | - |
CDP-choline | cosubstrate 1,2-dioleoyl-sn-glycerol, 23°C, pH 8.5 | Rattus norvegicus | |
| 0.0314 | - |
dihexadecanoylglycerol | 23°C, pH 8.5 | Rattus norvegicus | |
| 0.0405 | - |
bacterial 1,2-diacyl-sn-glycerol | 23°C, pH 8.5 | Rattus norvegicus | |
| 0.0501 | - |
dioctadecenoylglycerol | 23°C, pH 8.5 | Rattus norvegicus | |
| 0.0509 | - |
1,2-dioleoyl-sn-glycerol | 23°C, pH 8.5 | Rattus norvegicus | |
| 0.0681 | - |
dihexanoylglycerol | 23°C, pH 8.5 | Rattus norvegicus | |
| 0.118 | - |
dimyristoyl-sn-glycerol | 23°C, pH 8.5 | Rattus norvegicus | |
| 0.125 | 0.126 | 1-stearoyl-2-oleoyl-sn-glycerol | 23°C, pH 8.5 | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Rattus norvegicus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Rattus norvegicus | |
| Mn2+ | can partially replace Mg2+ in activation | Rattus norvegicus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adipocyte | - |
Rattus norvegicus | - |
| brain | - |
Rattus norvegicus | - |
| liver | - |
Rattus norvegicus | - |
| lung | fetal | Rattus norvegicus | - |
| mucosa | of intestine | Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CDP-choline + 1,2-diacylglycerol | - |
Rattus norvegicus | CMP + a phosphatidylcholine | - |
? | |
| CDP-choline + 1,2-dioleoyl-sn-glycerol | - |
Rattus norvegicus | CMP + 1,2-dioleoyl-sn-glycero-3-phosphocholine | - |
? | |
| CDP-choline + 1-stearoyl-2-oleoyl-sn-glycerol | - |
Rattus norvegicus | CMP + 1-stearoyl-2-oleoyl-sn-glycero-3-phosphocholine | - |
? | |
| CDP-choline + bacterial 1,2-diacyl-sn-glycerol | - |
Rattus norvegicus | CMP + phosphatidylethanolamine | - |
? | |
| CDP-choline + didecanoylglycerol | - |
Rattus norvegicus | CMP + didecanoylglycerophosphocholine | - |
? | |
| CDP-choline + didodecanoylglycerol | - |
Rattus norvegicus | CMP + didodecanoylglycerophosphocholine | - |
? | |
| CDP-choline + dihexanoylglycerol | - |
Rattus norvegicus | CMP + dihexanoylglycerophosphocholine | - |
? | |
| CDP-choline + dimyristoyl-sn-glycerol | - |
Rattus norvegicus | CMP + dimyristoyl-sn-glycerophosphocholine | - |
? | |
| additional information | specificity overview | Rattus norvegicus | ? | - |
? | |
| additional information | no substrate: 1,3-diC18:1-glycerol ether | Rattus norvegicus | ? | - |
? | |
| additional information | no substrate: 1,2-diC18:1-glycerol ether | Rattus norvegicus | ? | - |
? | |
| additional information | substrates are 1,2-diacylglycerols containing fatty acids 6, 12 and 14 carbons in length | Rattus norvegicus | ? | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
19 min, stable, microsomal preparation | Rattus norvegicus |
| 49 | - |
15 min, 50% loss of activity, microsomal preparation | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 9.3 | - |
Rattus norvegicus |
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