Literature summary for 2.7.8.17 extracted from

Franke, M.; Braulke, T.; Storch, S.
Transport of the GlcNAc-1-phosphotransferase alpha/beta-subunit precursor protein to the golgi apparatus requires a combinatorial sorting motif (2013), J. Biol. Chem., 288, 1238-1249.

Search for more literature for 2.7.8.17

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli, COS-7 cells and HeLa cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
K1236A/R1237A/K1238A	mutant containing a mutation of C-terminal endoplasmic reticulum export motif mainly co-localizes with the cis-Golgi marker protein but fails to co-distribute with the endoplasmic reticulum protein marker	Homo sapiens
K1236M	proteolytic cleavage of mutant is not affected. Mutant is localised mainly in the Golgi apparatus	Homo sapiens
K4Q	patient mutation K4Q impairs the endoplasmic reticulum export of the PT alpha/beta-subunit precursor protein. Mutant shows reduced levels of the PT beta-subunit. Mutant is localised in the Golgi apparatus and in the endoplasmic reticulum	Homo sapiens
L5A/L6A	replacement of the N-terminal dileucine motif with alanine residues results in a significant reduction of the PT alpha/beta-subunit precursor protein cleavage. Densitometric evaluation of intensities of immunoreactive bands show that the formation of the beta-subunit is reduced by 46% compared with the wild-type construct. Mutant mainly co-localizes with the cis-Golgi marker protein but fails to co-distribute with the endoplasmic reticulum protein marker	Homo sapiens
L5A/L6A/R1253A/I1254A/R1255A	double mutant containing a mutation of N-terminal and C-terminal endoplasmic reticulum export motif shows a strong inhibitory effect on cleavage of the PT alpha/beta-subunit precursor protein. Mutation leads to retention in the endoplasmic reticulum	Homo sapiens
L5A/L6A/R1253L/I1254L/R1255X	the transfer of the dileucine motif to the C-terminal domain replacing the dibasic-based motif 1253RIR1255 in combination with the substitution of the N-terminal dileucine motif, blocks the endoplasmic exit and the subsequent proteolytic cleavage to mature PT beta-subunit	Homo sapiens
L5R/L6R/R1253A/I1254A/R1255A	substitution of the N-terminal L-Leu/L-Leu motif by dibasic-based motifs RIR or RR in combination with alanine substitution of the C-terminal motif prevents the alpha/beta-subunit precursor protein from reaching the Golgi apparatus for cleavage	Homo sapiens
R1242A/R1243A/R1244A	by mutation of the arginine motif in beta subunit it is shown that this signal is not a functional endoplasmic reticulum retention signal	Homo sapiens
R1244A/R1245A/R1246A	mutant containing a mutation of C-terminal endoplasmic reticulum export motif mainly co-localizes with the cis-Golgi marker protein but fails to co-distribute with the endoplasmic reticulum protein marker	Homo sapiens
R1253A/I1254A/R1255A	mutant containing a mutation of C-terminal endoplasmic reticulum export motif mainly co-localizes with the cis-Golgi marker protein but fails to co-distribute with the endoplasmic reticulum protein marker	Homo sapiens
R925A	mutation results in an uncleavable PT alpha/beta-subunit precursor protein. Mutant co-localizes mainly with the cis-Golgi marker protein, no detection in endoplasmic reticulum	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
endoplasmic reticulum	PT alpha/beta-subunit precursor protein is exported into the Golgi apparatus whereas coexpressed separate alpha- and beta-subunits fail to reach the cis-Golgi compartment. Endoplasmic reticulum exit of the PT alpha/beta-subunit precursor protein is dependent on Sar-1 protein	Homo sapiens	5783	-
Golgi apparatus	uncleaved and cleaved forms of the PT alpha/beta-subunit precursor are localized in the Golgi apparatus	Homo sapiens	5794	-

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q3T906	-	-

Synonyms

Synonyms	Comment	Organism
N-acetylglucosamine-1-phosphotransferase	-	Homo sapiens
PT alpha/beta subunit precursor protein	-	Homo sapiens

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Release 2023.1 (January 2023)