Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
DELTA1-47 | N-terminal region (residues 1-47) of Saccharomyces cerevisiae Alg14 localizes its green fluorescent protein fusion to the endoplasmic reticulum membrane. Coimmunoprecipitation demonstrates that the N-terminal region of Alg14 is required for direct interaction with Alg7 | Saccharomyces cerevisiae |
G163A/G165A | mutant fails to rescue a loss of Alg14 function. Mutant is inactivated by loss of conserved G-loop | Saccharomyces cerevisiae |
V131A/I135A/V139A/V143A/V146A/F150A | a mutant in which six hydrophobic residues are replaced with L-Ala is able to rescue the loss of Alg14 function, indicating that the mutated hydrophobic residues do not have a deleterious effect on Alg14 activity. Growth of these cells is extremely slow at 30°C | Saccharomyces cerevisiae |
V131I/I135L/V139I/V143I/V146I/F150L | a mutant in which six hydrophobic residues are replaced with L-Ala is able to rescue the loss of Alg14 function | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | N-terminal region of Alg14 is respnsible for its localization to the endoplasmic reticulum membrane | Saccharomyces cerevisiae | 5783 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
Alg14 | - |
Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
malfunction | deletion of the Alg14 N-terminal region causes a severe growth defect at high temperature. Malfunction can be partially complemented by overexpression of Alg7 | Saccharomyces cerevisiae |