BRENDA - Enzyme Database
show all sequences of 2.7.8.17

Purification and characterization of human lymphoblast N-acetylglucosamine-1-phosphotransferase

Zhao, K.W.; Yeh, R.; Miller, A.L.; Glycobiology 2, 119-125 (1992)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
Tergitol NP-10
required
Homo sapiens
Inhibitors
Inhibitors
Commentary
Organism
Structure
phosphatidic acid
-
Homo sapiens
phosphatidylglycerol
-
Homo sapiens
phosphatidylserine
-
Homo sapiens
UDP
-
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.024
-
UDP-GlcNAc
-
Homo sapiens
0.117
-
methyl-alpha-D-mannoside
-
Homo sapiens
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
divalent cation Mn2+ or Mg2+ is required
Homo sapiens
Mn2+
divalent cation, Mn2+ or Mg2+ is required
Homo sapiens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1000000
-
-
Homo sapiens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
lymphoblast
-
Homo sapiens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-N-acetyl-D-glucosamine + methyl-alpha-D-mannoside
-
645369
Homo sapiens
UMP + N-acetyl-D-glucosamine-phospho-(methyl-alpha-D-mannoside)
-
-
-
-
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
7.5
-
Homo sapiens
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
Tergitol NP-10
required
Homo sapiens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
phosphatidic acid
-
Homo sapiens
phosphatidylglycerol
-
Homo sapiens
phosphatidylserine
-
Homo sapiens
UDP
-
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.024
-
UDP-GlcNAc
-
Homo sapiens
0.117
-
methyl-alpha-D-mannoside
-
Homo sapiens
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
divalent cation Mn2+ or Mg2+ is required
Homo sapiens
Mn2+
divalent cation, Mn2+ or Mg2+ is required
Homo sapiens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1000000
-
-
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
lymphoblast
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-N-acetyl-D-glucosamine + methyl-alpha-D-mannoside
-
645369
Homo sapiens
UMP + N-acetyl-D-glucosamine-phospho-(methyl-alpha-D-mannoside)
-
-
-
-
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
7.5
-
Homo sapiens
Other publictions for EC 2.7.8.17
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738354
van Meel
Mucolipidosis III GNPTG missen ...
Homo sapiens
Hum. Mutat.
37
623-626
2016
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1
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4
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1
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1
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1
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1
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-
-
-
-
2
2
-
-
-
738704
van Meel
Multiple domains of GlcNAc-1-p ...
Homo sapiens
J. Biol. Chem.
291
8295-8307
2016
-
-
1
-
1
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1
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1
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1
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2
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1
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1
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1
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2
2
-
-
-
-
-
-
-
-
-
1
1
-
-
-
739081
Ko
AAV8-mediated expression of N- ...
Mus musculus
Mol. Genet. Metab.
117
447-455
2016
-
1
1
-
1
-
-
-
-
-
-
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3
-
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-
-
-
-
-
-
-
-
-
1
1
-
-
-
738351
Velho
Analyses of disease-related GN ...
Homo sapiens
Hum. Mol. Genet.
24
3497-3505
2015
-
-
1
-
6
-
-
-
3
-
3
1
-
2
-
2
-
-
-
-
-
-
2
2
1
-
-
-
1
-
-
-
-
-
-
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-
1
-
-
6
-
-
-
-
-
3
-
3
1
-
-
2
-
-
-
-
-
2
2
1
-
-
-
1
-
-
-
-
2
2
-
-
-
738667
Qian
Analysis of mucolipidosis II/I ...
Danio rerio, Homo sapiens
J. Biol. Chem.
290
3045-3056
2015
-
-
2
-
35
-
-
-
2
2
-
2
-
4
-
-
-
-
-
-
-
-
2
2
2
-
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2
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2
-
-
35
-
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2
2
-
2
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-
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2
2
2
-
-
-
2
-
-
-
-
6
6
-
-
-
739732
Blanz
Mannose 6-phosphate-independen ...
Homo sapiens, Mus musculus
Traffic
16
1127-1136
2015
-
-
-
-
1
-
-
-
2
-
-
4
-
4
-
2
-
-
-
2
-
-
4
-
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1
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2
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4
-
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2
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2
-
-
4
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
739540
van Meel
Mislocalization of phosphotran ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
111
3532-3537
2014
-
-
1
-
3
-
-
-
2
-
3
1
-
2
-
1
-
-
-
-
-
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
2
-
3
1
-
-
1
-
-
-
-
-
2
1
1
-
-
-
1
-
-
-
-
2
2
-
-
-
725513
Franke
Transport of the GlcNAc-1-phos ...
Homo sapiens
J. Biol. Chem.
288
1238-1249
2013
-
-
1
-
11
-
-
-
2
-
-
-
-
2
-
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1
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-
11
-
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2
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-
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-
739527
Qian
The DMAP interaction domain of ...
Homo sapiens
Proc. Natl. Acad. Sci. USA
110
10246-10251
2013
-
-
1
-
1
-
-
-
1
-
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1
-
2
-
1
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3
1
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1
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1
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1
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1
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1
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3
1
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-
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-
-
-
-
2
2
-
-
-
722362
Lu
Alg14 organizes the formation ...
Saccharomyces cerevisiae
Glycobiology
22
504-516
2012
-
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1
-
4
-
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-
1
-
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1
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1
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1
1
-
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-
725457
Encarnacao
Post-translational modificatio ...
Homo sapiens
J. Biol. Chem.
286
5311-5318
2011
-
-
1
-
11
-
-
-
1
-
1
-
-
3
-
1
-
-
-
-
-
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2
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1
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11
-
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1
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1
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1
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-
-
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2
-
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-
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-
724169
Qian
UDP-GlcNAc:Glycoprotein N-acet ...
Dictyostelium discoideum
Biochem. Biophys. Res. Commun.
393
678-681
2010
-
-
-
-
-
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2
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1
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1
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1
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
725407
Qian
Functions of the alpha, beta, ...
Homo sapiens
J. Biol. Chem.
285
3360-3370
2010
-
-
1
-
-
-
1
15
-
-
2
-
-
3
-
-
-
1
-
-
-
-
9
-
1
-
-
18
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
15
-
-
2
-
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-
-
-
-
-
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9
-
1
-
-
18
1
-
-
-
-
1
1
-
15
15
671199
Kudo
Mucolipidosis II (I-cell disea ...
Homo sapiens
Am. J. Hum. Genet.
78
451-463
2006
-
-
1
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1
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2
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1
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1
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2
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1
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674538
Kudo
Structural requirements for ef ...
Homo sapiens
J. Biol. Chem.
281
11761-11768
2006
-
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1
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-
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2
-
1
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3
-
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1
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1
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1
1
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1
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2
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1
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1
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1
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1
1
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661819
Sun
Overexpression of mouse GlcNAc ...
Mus musculus
Gene
347
55-64
2005
-
-
1
-
-
-
-
-
1
-
-
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2
-
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1
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662410
Steet
Identification of the minimal ...
Homo sapiens
J. Biol. Chem.
280
33318-33323
2005
-
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1
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1
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662418
Kudo
The alpha- and beta-subunits o ...
Homo sapiens
J. Biol. Chem.
280
36141-36149
2005
-
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1
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1
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3
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1
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645377
Schierau
Interaction of arylsulfatase A ...
Mus musculus
J. Biol. Chem.
274
3651-3658
1999
-
-
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1
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1
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645380
Lukong
Identification of UDP-N-acetyl ...
Rattus norvegicus
Biochemistry
38
73-80
1999
-
-
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1
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1
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1
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3
1
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1
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1
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3
1
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645378
Bao
Bovine UDP-N-acetylglucosamine ...
Bos taurus
J. Biol. Chem.
271
31446-31451
1996
2
-
-
-
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8
6
-
2
3
-
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1
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1
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1
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5
1
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1
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1
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2
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8
1
6
-
2
3
-
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1
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1
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5
1
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1
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-
645379
Bao
Bovine UDP-N-acetylglucosamine ...
Bos taurus
J. Biol. Chem.
271
31437-31445
1996
-
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1
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1
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1
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1
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1
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1
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645376
Guillen
-
UDP-N-acetylglucosamine: glyco ...
Ceratitis capitata, Ceratitis capitata ARG-17
Insect Biochem. Mol. Biol.
24
213-219
1994
-
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3
1
2
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2
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1
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1
1
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1
1
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1
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1
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1
1
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1
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