Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Dolichyl-phosphoryl-mannose | stimulates | Sus scrofa | |
GDP-mannose | stimulates by protecting the substrate UDP-GlcNAc from degradation | Sus scrofa | |
phosphatidylglycerol | solubilized enzyme is stimulated by exogenously added phospholipids in order of increasing activity: phosphatidylglycerol, phosphatidylinositol, phosphatidylserine | Sus scrofa | |
phosphatidylinositol | solubilized enzyme is stimulated by exogenously added phospholipids in order of increasing activity: phosphatidylglycerol, phosphatidylinositol, phosphatidylserine | Sus scrofa | |
phosphatidylserine | solubilized enzyme is stimulated by exogenously added phospholipids in order of increasing activity: phosphatidylglycerol, phosphatidylinositol, phosphatidylserine | Sus scrofa | |
Phospholipid | solubilized enzyme is stimulated by exogenously added phospholipids in order of increasing activity: phosphatidylglycerol, phosphatidylinositol, phosphatidylserine | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NEM | protected to the extent of about 50% when all of the substrates, UDP-GlcNAc, dolichyl phosphate and Mn2+ are added before addition of the sulfhydryl reagent | Sus scrofa | |
PCMB | protected to the extent of about 50% when all of the substrates, UDP-GlcNAc, dolichyl phosphate anf Mn2+ are added before addition of the sulfhydryl reagent | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0001 | - |
UDP-GlcNAc | pH 7.5, 37°C | Sus scrofa | |
0.001 | - |
dolichyl phosphate | pH 7.5, 37°C | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Sus scrofa | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | less effective than Mn2+ or Mg2+ | Sus scrofa | |
Mg2+ | either Mn2+, 1 mM, or Mg2+, 10 mM, required for optimal activity | Sus scrofa | |
Mn2+ | either Mn2+, 1 mM, or Mg2+, 10 mM, required for optimal activity | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
aorta | - |
Sus scrofa | - |
Storage Stability | Organism |
---|---|
0-4°C, 20% glycerol, 0.02 mg phosphatidylglycerol/mg of protein, less than 20% loss of activity after 6 days. In absence of both glycerol and phosphatidylglycerol the enzyme is quite unstable and loses most of its activity within 24 h | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate | - |
Sus scrofa | UMP + N-acetyl-alpha-D-glucosaminyl-diphosphodolichol | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | 7.6 | - |
Sus scrofa |