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Literature summary for 2.7.8.15 extracted from

  • Shailubhai, K.; Dong-Yu, B.; Saxena, E.S.; Vijay, I.K.
    Purification and characterization of UDP-N-acetyl-D-glucosamine:dolichol phosphate N-acetyl-D-glucosamine-1-phosphate transferase involved in the biosynthesis of asparagine-linked glycoproteins in the mammary gland (1988), J. Biol. Chem., 263, 15964-15972.
    View publication on PubMed
Search for more literature for 2.7.8.15

Activating Compound

Activating Compound Comment Organism Structure
Dolichyl-phosphoryl-mannose stimulates Bos taurus

General Stability

General Stability Organism
purified enzyme is resistant to digestion with either endo-beta-N-acetylglucosaminidase H or F Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
Diumycin
-
 Bos taurus
dolichyl phosphate at high concentrations Bos taurus
Hg2+ partially reversed by dithiothreitol Bos taurus
iodoacetamide
-
 Bos taurus
PCMB
-
 Bos taurus
tunicamycin
-
 Bos taurus
UDP
-
 Bos taurus
UDP-glucose
-
 Bos taurus
UDP-hexanolamine
-
 Bos taurus
UDP-xylose
-
 Bos taurus
UMP
-
 Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0045
-
 UDP-GlcNAc pH 7.6, 37°C Bos taurus
0.016
-
 dolichyl phosphate pH 7.6, 37°C Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Bos taurus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ stimulates Bos taurus
Mn2+ stimulates Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
330000 360000 large molecular mass of the enzyme can be due to aggregation of the hydrophobic, membrane-derived enzyme rather than resulting from subunit interaction, gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-D-glucosamine + dolichyl phosphate Bos taurus enzyme initiates the dolichol cycle for the biosynthesis of asparagine-linked glycoproteins ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information the enzyme is not a glycoprotein of the asparagine-linked type Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
mammary gland lactating Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Bos taurus

Storage Stability

Storage Stability Organism
4°C, solubilized enzyme loses most of its activity within 12 h Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate
-
 Bos taurus UMP + N-acetyl-alpha-D-glucosaminyl-diphosphodolichol
-
 ?
UDP-N-acetyl-D-glucosamine + dolichyl phosphate enzyme initiates the dolichol cycle for the biosynthesis of asparagine-linked glycoproteins Bos taurus ?
-
 ?

Subunits

Subunits Comment Organism
More results indicate that either 70000 MW band in SDS-PAGE is a precursor form of the enzyme or this polypeptide, representing the native enzyme or its subunit, is proteolyzed to smaller, enzymatically active peptides of 50000 Da and 46000 Da during purification Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4 7.6
-
 Bos taurus