BRENDA - Enzyme Database
show all sequences of 2.7.8.15

Purification and characterization of UDP-N-acetyl-D-glucosamine:dolichol phosphate N-acetyl-D-glucosamine-1-phosphate transferase involved in the biosynthesis of asparagine-linked glycoproteins in the mammary gland

Shailubhai, K.; Dong-Yu, B.; Saxena, E.S.; Vijay, I.K.; J. Biol. Chem. 263, 15964-15972 (1988)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
Dolichyl-phosphoryl-mannose
stimulates
Bos taurus
General Stability
General Stability
Organism
purified enzyme is resistant to digestion with either endo-beta-N-acetylglucosaminidase H or F
Bos taurus
Inhibitors
Inhibitors
Commentary
Organism
Structure
Diumycin
-
Bos taurus
dolichyl phosphate
at high concentrations
Bos taurus
Hg2+
partially reversed by dithiothreitol
Bos taurus
iodoacetamide
-
Bos taurus
PCMB
-
Bos taurus
tunicamycin
-
Bos taurus
UDP
-
Bos taurus
UDP-glucose
-
Bos taurus
UDP-hexanolamine
-
Bos taurus
UDP-xylose
-
Bos taurus
UMP
-
Bos taurus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0045
-
UDP-GlcNAc
pH 7.6, 37°C
Bos taurus
0.016
-
dolichyl phosphate
pH 7.6, 37°C
Bos taurus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Bos taurus
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
stimulates
Bos taurus
Mn2+
stimulates
Bos taurus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
330000
360000
large molecular mass of the enzyme can be due to aggregation of the hydrophobic, membrane-derived enzyme rather than resulting from subunit interaction, gel filtration
Bos taurus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-N-acetyl-D-glucosamine + dolichyl phosphate
Bos taurus
enzyme initiates the dolichol cycle for the biosynthesis of asparagine-linked glycoproteins
?
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bos taurus
-
-
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
additional information
the enzyme is not a glycoprotein of the asparagine-linked type
Bos taurus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
mammary gland
lactating
Bos taurus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Bos taurus
Storage Stability
Storage Stability
Organism
4°C, solubilized enzyme loses most of its activity within 12 h
Bos taurus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate
-
645350
Bos taurus
UMP + N-acetyl-alpha-D-glucosaminyl-diphosphodolichol
-
645350
Bos taurus
?
UDP-N-acetyl-D-glucosamine + dolichyl phosphate
enzyme initiates the dolichol cycle for the biosynthesis of asparagine-linked glycoproteins
645350
Bos taurus
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
More
results indicate that either 70000 MW band in SDS-PAGE is a precursor form of the enzyme or this polypeptide, representing the native enzyme or its subunit, is proteolyzed to smaller, enzymatically active peptides of 50000 Da and 46000 Da during purification
Bos taurus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
7.6
-
Bos taurus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
Dolichyl-phosphoryl-mannose
stimulates
Bos taurus
General Stability (protein specific)
General Stability
Organism
purified enzyme is resistant to digestion with either endo-beta-N-acetylglucosaminidase H or F
Bos taurus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Diumycin
-
Bos taurus
dolichyl phosphate
at high concentrations
Bos taurus
Hg2+
partially reversed by dithiothreitol
Bos taurus
iodoacetamide
-
Bos taurus
PCMB
-
Bos taurus
tunicamycin
-
Bos taurus
UDP
-
Bos taurus
UDP-glucose
-
Bos taurus
UDP-hexanolamine
-
Bos taurus
UDP-xylose
-
Bos taurus
UMP
-
Bos taurus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0045
-
UDP-GlcNAc
pH 7.6, 37°C
Bos taurus
0.016
-
dolichyl phosphate
pH 7.6, 37°C
Bos taurus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Bos taurus
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
stimulates
Bos taurus
Mn2+
stimulates
Bos taurus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
330000
360000
large molecular mass of the enzyme can be due to aggregation of the hydrophobic, membrane-derived enzyme rather than resulting from subunit interaction, gel filtration
Bos taurus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-N-acetyl-D-glucosamine + dolichyl phosphate
Bos taurus
enzyme initiates the dolichol cycle for the biosynthesis of asparagine-linked glycoproteins
?
-
-
?
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
additional information
the enzyme is not a glycoprotein of the asparagine-linked type
Bos taurus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
mammary gland
lactating
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Bos taurus
Storage Stability (protein specific)
Storage Stability
Organism
4°C, solubilized enzyme loses most of its activity within 12 h
Bos taurus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate
-
645350
Bos taurus
UMP + N-acetyl-alpha-D-glucosaminyl-diphosphodolichol
-
645350
Bos taurus
?
UDP-N-acetyl-D-glucosamine + dolichyl phosphate
enzyme initiates the dolichol cycle for the biosynthesis of asparagine-linked glycoproteins
645350
Bos taurus
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
More
results indicate that either 70000 MW band in SDS-PAGE is a precursor form of the enzyme or this polypeptide, representing the native enzyme or its subunit, is proteolyzed to smaller, enzymatically active peptides of 50000 Da and 46000 Da during purification
Bos taurus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
7.6
-
Bos taurus
Other publictions for EC 2.7.8.15
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737859
Kim
The UDP-N-acetylglucosamine:do ...
Solanum tuberosum
Biosci. Biotechnol. Biochem.
77
1589-1592
2013
-
2
1
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
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-
2
1
-
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-
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-
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-
-
-
-
-
703322
Bretthauer
Structure, expression, and reg ...
Bos taurus, Cricetulus griseus, Homo sapiens, Mus musculus, Rattus norvegicus
Curr. Drug Targets
10
477-482
2009
-
-
5
-
1
-
5
-
5
-
8
5
-
5
-
-
5
-
-
5
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
1
-
-
5
-
-
5
-
8
5
-
-
-
5
-
5
-
-
5
-
-
-
-
-
-
-
-
-
-
5
5
-
-
-
691880
Plante
Mucolipidosis II: a single cau ...
Homo sapiens
Clin. Genet.
73
236-244
2008
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
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1
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-
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-
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-
-
-
-
-
-
-
673891
Gao
Unexpected basis for impaired ...
Cricetulus griseus, Mesocricetus auratus
Glycobiology
18
125-134
2007
-
-
-
-
-
-
2
-
1
-
-
-
-
2
-
-
-
-
-
1
-
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3
-
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2
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1
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1
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3
-
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-
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-
-
-
661252
Mendelsohn
A hypomorphic allele of the fi ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
1723
33-44
2005
-
-
-
-
-
-
-
-
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1
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2
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1
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645357
Sorensen
Characterisation of the gptA g ...
Aspergillus niger, Aspergillus niger N402
Biochim. Biophys. Acta
1619
89-97
2003
-
-
1
-
-
-
-
-
-
-
1
2
-
6
-
-
-
-
-
-
-
-
4
1
-
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-
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-
1
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1
2
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-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
645362
Wu
Deficiency of UDP-GlcNAc: doli ...
Homo sapiens
Hum. Mutat.
22
144-150
2003
-
-
-
-
1
-
-
-
-
-
-
1
-
2
-
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2
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1
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1
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-
2
-
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-
-
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-
-
-
-
-
-
-
-
-
-
645363
Meissner
Regulation of UDP-N-acetylgluc ...
Mus musculus
Biochem. J.
338
561-568
1999
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
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1
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2
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1
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2
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-
-
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-
645360
Huang
Characterization of multiple t ...
Cricetulus griseus
Mol. Cell. Biochem.
181
97-106
1998
-
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3
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1
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1
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1
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-
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-
645361
Scocca
Aspartic acid 252 and asparagi ...
Cricetulus griseus
Glycobiology
7
1181-1191
1997
-
-
1
-
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-
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1
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2
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2
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2
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-
645367
Dan
Oligomerization of hamster UDP ...
Cricetulus griseus
J. Biol. Chem.
272
14214-14219
1997
-
-
-
-
1
-
-
-
2
-
1
-
-
1
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1
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1
1
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1
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1
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1
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1
1
-
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-
645366
Dan
Hamster UDP-N-acetylglucosamin ...
Cricetulus griseus
J. Biol. Chem.
271
30717-30724
1996
-
-
-
-
-
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-
-
1
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1
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2
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1
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-
-
-
645356
Zeng
UDP-N-acetylglucosamine:dolich ...
Glycine max
Eur. J. Biochem.
233
458-466
1995
2
-
-
-
-
-
4
-
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-
1
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-
2
-
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-
1
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1
1
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2
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2
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4
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1
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1
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1
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
645358
Kukuruzinska
Diminished activity of the fir ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
1247
51-59
1995
-
-
-
-
-
-
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1
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3
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2
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645359
Mota
Developmental regulation and t ...
Cricetulus griseus
Biochem. Biophys. Res. Commun.
204
284-291
1994
-
-
-
-
15
-
-
-
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-
1
1
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2
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7
-
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2
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15
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1
1
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7
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2
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645364
Zara
Role of the carboxyl terminus ...
Cricetulus griseus
J. Biol. Chem.
269
19108-19115
1994
-
-
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4
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1
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1
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1
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1
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2
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4
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1
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1
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1
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2
-
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-
645365
Rajput
Structure and organization of ...
Mus musculus
J. Biol. Chem.
269
9590-9597
1994
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5
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2
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645355
Chandra
Specific lipids enhance the ac ...
Rattus norvegicus
Arch. Biochem. Biophys.
290
345-354
1991
3
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2
1
1
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1
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1
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1
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2
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3
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1
1
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1
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