Literature summary for 2.7.8.13 extracted from

Liu, Y.; Moura, E.C.C.M.; Doerr, J.M.; Scheidelaar, S.; Heger, M.; Egmond, M.R.; Killian, J.A.; Mohammadi, T.; Breukink, E.
Bacillus subtilis MraY in detergent-free system of nanodiscs wrapped by styrene-maleic acid copolymers (2018), PLoS ONE, 13, e0206692 .

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Application

Application	Comment	Organism
synthesis	heterologous production of enzyme in Escherichia coli membranes into styrene-maleic acid-wrapped nanodiscs and purification using detergent-free styrene-maleic acid copolymer system. Yield and the purity of the recombinant protein are comparable to enzyme extracted with a conventional detergent. The predominantly alpha-helical secondary structure of the protein in SMA-wrapped nanodiscs is more stable against heat denaturation compared to the micellar protein	Bacillus subtilis

Cloned(Commentary)

Cloned (Comment)	Organism
heterologous production in Escherichia coli membranes into styrene-maleic acid-wrapped nanodiscs	Bacillus subtilis

Organism

Organism	UniProt	Comment	Textmining
Bacillus subtilis	Q03521	-	-
Bacillus subtilis 168	Q03521	-	-

Purification (Commentary)

Purification (Comment)	Organism
purification using detergent-free styrene-maleic acid copolymer system	Bacillus subtilis

Synonyms

Synonyms	Comment	Organism
MraY	-	Bacillus subtilis

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Release 2023.1 (January 2023)