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Literature summary for 2.7.8.13 extracted from

  • Heydanek, M.G.; Neuhaus, F.C.
    The initial stage in peptidoglycan synthesis. IV. Solubilization of phospho-N-acetylmuramyl-pentapeptide translocase (1969), Biochemistry, 8, 1474-1481.
    View publication on PubMed

General Stability

General Stability Organism
only 2.5% of the activity can be recovered after dialysis Staphylococcus aureus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Staphylococcus aureus 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000 200000 second peak of exchange activity, low molecular weight form, gel filtration Staphylococcus aureus
2000000
-
major peak of activity, high molecular weight form, gel filtration Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-NAc-muramoyl-pentapeptide + acceptor Staphylococcus aureus initial stage in peptidoglycan synthesis, key role in selecting analogs of UDP-MurNAc-Ala-gammaDGlu-Lys-DAla-DAla, membrane associated reaction of cell wall biosynthesis UMP + acceptor-phospho-NAc-muramoyl-pentapeptide
-
r
UDP-NAc-muramoyl-pentapeptide + acceptor Staphylococcus aureus biosynthesis of peptidoglycan, first lipid intermediate UMP + acceptor-phospho-NAc-muramoyl-pentapeptide
-
r
UDP-NAc-muramoyl-pentapeptide + acceptor Staphylococcus aureus Copenhagen initial stage in peptidoglycan synthesis, key role in selecting analogs of UDP-MurNAc-Ala-gammaDGlu-Lys-DAla-DAla, membrane associated reaction of cell wall biosynthesis UMP + acceptor-phospho-NAc-muramoyl-pentapeptide
-
r
UDP-NAc-muramoyl-pentapeptide + acceptor Staphylococcus aureus Copenhagen biosynthesis of peptidoglycan, first lipid intermediate UMP + acceptor-phospho-NAc-muramoyl-pentapeptide
-
r

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
Copenhagen
-
Staphylococcus aureus Copenhagen
-
Copenhagen
-

Purification (Commentary)

Purification (Comment) Organism
low molecular weight form Staphylococcus aureus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
specific activity of the enzyme increases 4fold by late-logarithmic phase Staphylococcus aureus

Storage Stability

Storage Stability Organism
-196°C, retains complete activity for at least 2 months Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetylmuramoyl-pentapeptide + [2H]UMP
-
Staphylococcus aureus UMP + [2H]UDP-NAc-muramoyl-pentapeptide
-
r
UDP-N-acetylmuramoyl-pentapeptide + [2H]UMP
-
Staphylococcus aureus Copenhagen UMP + [2H]UDP-NAc-muramoyl-pentapeptide
-
r
UDP-NAc-muramoyl-pentapeptide + acceptor
-
Staphylococcus aureus UMP + acceptor-phospho-NAc-muramoyl-pentapeptide
-
r
UDP-NAc-muramoyl-pentapeptide + acceptor initial stage in peptidoglycan synthesis, key role in selecting analogs of UDP-MurNAc-Ala-gammaDGlu-Lys-DAla-DAla, membrane associated reaction of cell wall biosynthesis Staphylococcus aureus UMP + acceptor-phospho-NAc-muramoyl-pentapeptide
-
r
UDP-NAc-muramoyl-pentapeptide + acceptor biosynthesis of peptidoglycan, first lipid intermediate Staphylococcus aureus UMP + acceptor-phospho-NAc-muramoyl-pentapeptide
-
r
UDP-NAc-muramoyl-pentapeptide + acceptor
-
Staphylococcus aureus Copenhagen UMP + acceptor-phospho-NAc-muramoyl-pentapeptide
-
r
UDP-NAc-muramoyl-pentapeptide + acceptor initial stage in peptidoglycan synthesis, key role in selecting analogs of UDP-MurNAc-Ala-gammaDGlu-Lys-DAla-DAla, membrane associated reaction of cell wall biosynthesis Staphylococcus aureus Copenhagen UMP + acceptor-phospho-NAc-muramoyl-pentapeptide
-
r
UDP-NAc-muramoyl-pentapeptide + acceptor biosynthesis of peptidoglycan, first lipid intermediate Staphylococcus aureus Copenhagen UMP + acceptor-phospho-NAc-muramoyl-pentapeptide
-
r

pI Value

Organism Comment pI Value Maximum pI Value
Staphylococcus aureus electrofocusing in a pH gradient 3-8 containing 8 M urea
-
4.9