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Literature summary for 2.7.8.12 extracted from
- Two conserved histidine residues are critical to the function of the TagF-like family of enzymes (2005), J. Biol. Chem., 280, 36683-36690.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D630A | the ratio of turnover number to KM-value is 3.3fold lower than the wild-type ratio | Bacillus subtilis |
| D645A | the ratio of turnover number to KM-value is 3.5fold lower than the wild-type ratio | Bacillus subtilis |
| E604A | the ratio of turnover number to KM-value is 1.3fold lower than the wild-type ratio | Bacillus subtilis |
| E639A | the ratio of turnover number to KM-value is 3.9fold lower than the wild-type ratio | Bacillus subtilis |
| H474A | the ratio of turnover number to KM-value is 4900fold lower than the wild-type ratio | Bacillus subtilis |
| H612A | the ratio of turnover number to KM-value is more than 4900fold lower than the wild-type ratio | Bacillus subtilis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.23 | - |
CDP-glycerol | wild-type enzyme | Bacillus subtilis |  |
| 0.23 | - |
CDP-glycerol | mutant enzyme E604A | Bacillus subtilis | |
| 0.39 | - |
CDP-glycerol | mutant enzyme D630A | Bacillus subtilis | |
| 0.7 | - |
CDP-glycerol | mutant enzyme E639A | Bacillus subtilis | |
| 0.75 | - |
CDP-glycerol | mutant enzyme D645A | Bacillus subtilis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CDP-glycerol + (glycerophosphate)n | Bacillus subtilis | the enzyme is responsible for the synthesis of wall teichoic acid | CMP + (glycerophosphate)n+1 | - |
? | |
| CDP-glycerol + (glycerophosphate)n | Bacillus subtilis 168 | the enzyme is responsible for the synthesis of wall teichoic acid | CMP + (glycerophosphate)n+1 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
| Bacillus subtilis 168 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus subtilis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, the carboxy-terminal His6-tagged TagF protein is stable for several months | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CDP-glycerol + (glycerophosphate)n | the enzyme is responsible for the synthesis of wall teichoic acid | Bacillus subtilis | CMP + (glycerophosphate)n+1 | - |
? | |
| CDP-glycerol + (glycerophosphate)n | the enzyme is responsible for the synthesis of wall teichoic acid | Bacillus subtilis 168 | CMP + (glycerophosphate)n+1 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| poly(glycerol phosphate)polymerase | - |
Bacillus subtilis |
| TagF protein | - |
Bacillus subtilis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.12 | - |
CDP-glycerol | mutant enzyme D630A | Bacillus subtilis | |
| 0.167 | - |
CDP-glycerol | mutant enzyme E604A | Bacillus subtilis | |
| 0.167 | - |
CDP-glycerol | mutant enzyme E639A | Bacillus subtilis | |
| 0.217 | - |
CDP-glycerol | mutant enzyme D645A | Bacillus subtilis | |
| 0.233 | - |
CDP-glycerol | wild-type enzyme | Bacillus subtilis | |
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Release 2023.1 (January 2023)
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