Literature summary for 2.7.8.11 extracted from

Liu, X.; Zhai, S.; Zhao, Y.; Sun, B.; Liu, C.; Yang, A.; Zhang, J.
Overexpression of the phosphatidylinositol synthase gene (ZmPIS) conferring drought stress tolerance by altering membrane lipid composition and increasing ABA synthesis in maize (2013), Plant Cell Environ., 36, 1037-1055.

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Cloned(Commentary)

Cloned (Comment)	Organism
gene ZmPIS, cloning of the gene ZmPIS in sense or antisense orientation in the T-DNA region of the vector, driven by a maize-constitutive ubiquitin promoter, overexpression of the enzyme in transgenic Zea mays, and quantitative RT-PCR expression analysis	Zea mays

Protein Variants

Protein Variants	Comment	Organism
additional information	generation of transgenic maize constitutively overexpressing or underexpressing gene ZmPIS, encoding PIS from maize, under the control of a maize ubiquitin promoter. Lipid profiling analysis shows that, under drought stress conditions, the overexpression of ZmPIS in maize results in significantly elevated levels of most phospholipids and galactolipids in leaves compared with those in wild-type plants, analysis of expression pattern of genes involved in the phospholipid metabolism pathway and drought response, phenotype, overview. Sense transgenic plants are more tolerant to drought stress at the pre-flowering stage and retain more solutes and water under drought stress conditions	Zea mays

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Zea mays	16020	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
CDP-diacylglycerol + myo-inositol	Zea mays	-	CMP + 1-phosphatidyl-1D-myo-inositol	-	?
CDP-diacylglycerol + myo-inositol	Zea mays DH4866	-	CMP + 1-phosphatidyl-1D-myo-inositol	-	?

Organism

Organism	UniProt	Comment	Textmining
Zea mays	Q6UG58	-	-
Zea mays DH4866	Q6UG58	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Zea mays	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
CDP-diacylglycerol + myo-inositol	-	Zea mays	CMP + 1-phosphatidyl-1D-myo-inositol	-	?
CDP-diacylglycerol + myo-inositol	-	Zea mays DH4866	CMP + 1-phosphatidyl-1D-myo-inositol	-	?

Synonyms

Synonyms	Comment	Organism
phosphatidylinositol synthase	-	Zea mays
PtdIns synthase	-	Zea mays
ZmPIS	-	Zea mays

General Information

General Information	Comment	Organism
malfunction	overexpression of the phosphatidylinositol synthase gene (ZmPIS) confers drought stress tolerance by altering membrane lipid composition and increasing abscisic acid synthesis in maize. Lipid profiling analysis shows that, under drought stress conditions, the overexpression of ZmPIS in maize results in significantly elevated levels of most phospholipids and galactolipids in leaves compared with those in wild-type. The expression of some genes involved in the phospholipid metabolism pathway and the abscisic acid biosynthesis pathway including ZmPLC, ZmPLD, ZmDGK1, ZmDGK3, ZmPIP5K9, ZmABA1, ZmNCED, ZmAAO1, ZmAAO2 and ZmSCA1, is markedly upregulated in the overexpression lines after drought stress. The drought stress tolerance of the ZmPIS sense transgenic plants is enhanced significantly at the preflowering stages compared with wild-type maize plants	Zea mays
metabolism	phosphatidylinositol synthase is a key enzyme in the phospholipid pathway, it catalyzes the first step of the phosphatidylinositol signalling pathway	Zea mays
physiological function	the enzyme is responsible for phosphatidylinositol synthesis, which is not only a structural component of cell membranes, but also the precursor of the phospholipid signal molecules that regulate plant response to environment stresses. ZmPIS regulates the plant response to drought stress through altering membrane lipid composition and increasing abscisic acid synthesis in maize	Zea mays

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Release 2023.1 (January 2023)