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Literature summary for 2.7.8.11 extracted from
- Characterisation of the phosphatidylinositol synthase gene of Plasmodium species (2007), Res. Microbiol., 158, 51-59.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Plasmodium falciparum |
- |
Plasmodium knowlesi |
| highly spliced gene with 9 exons, cDNA encodes 207 amino acids, from mRNA in pCRII-Blunt-TOPO for cloning and sequencing (transformation of Escherichia coli TOP10 and DH5alpha) | Plasmodium knowlesi |
| highly spliced gene with 9 exons, cDNA encodes 209 amino acids, from total RNA in pCR-BluntII-Topo for cloning and sequencing (transformation of Escherichia coli TOP10 and DH5alpha), in p413 for complementation studies in Saccharomyces cerevisiae | Plasmodium falciparum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| Hy3 | hybrid construct of 57 N-terminal amino acids from Saccharomyces cerevisiae PIS and 159 C-terminal amino acids from PfPIS, no in vitro activity, 60% complementation of in vivo activity in yeast lacking endogenous PIS gene compared to ScPIS complementation efficiency (100%) | Plasmodium falciparum |
| Hy4 | hybrid construct of 14 N-terminal amino acids from Saccharomyces cerevisiae PIS and 202 C-terminal amino acids from PfPIS, 37% complementation of in vivo activity in yeast lacking endogenous PIS gene compared to ScPIS complementation efficiency (100%) | Plasmodium falciparum |
| additional information | gene successfully complements a Saccharomyces cerevisiae PIS1 deletion mutant | Plasmodium falciparum |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Plasmodium falciparum | 16020 | - |
| membrane | - |
Plasmodium knowlesi | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23735 | - |
x * 23735, calculated | Plasmodium knowlesi |
| 23740 | - |
theoretical | Plasmodium knowlesi |
| 23920 | - |
theoretical | Plasmodium falciparum |
| 23924 | - |
x * 23924, calculated | Plasmodium falciparum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Plasmodium falciparum | C0H5B7 | - |
- |
| Plasmodium falciparum | Q2MJB9 | - |
- |
| Plasmodium knowlesi | Q2MJB8 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
1.5 nmol/h/1x10(10) cells, wild-type, in culture phosphatidylinositol synthesis, intact yeast cells after overexpression | Plasmodium falciparum |
| additional information | - |
2.0 nmol/h/1x10(10) cells, hybrid Hy4, in culture phosphatidylinositol synthesis, intact yeast cells after overexpression | Plasmodium falciparum |
| additional information | - |
3.4 nmol/min per 1x10(10) infected red blood cells | Plasmodium falciparum |
| additional information | - |
3.7 nmol/h/1x10(10) cells, hybrid Hy3, in culture phosphatidylinositol synthesis, intact yeast cells after overexpression | Plasmodium falciparum |
| additional information | - |
no detectable activity for hybrid Hy3, in vitro phosphatidylinositol synthesis, yeast membrane extracts after overexpression | Plasmodium falciparum |
| 0.000023 | - |
wild-type, in vitro phosphatidylinositol synthesis, yeast membrane extracts after overexpression | Plasmodium falciparum |
| 0.000036 | - |
hybrid Hy4, in vitro phosphatidylinositol synthesis, yeast membrane extracts after overexpression | Plasmodium falciparum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CDP-diacylglycerol + myo-inositol | parasite membrane extracts, infected red blood cells | Plasmodium falciparum | CMP + phosphatidyl-myo-inositol | - |
? |  |
| additional information | partial complementation of PIS activity by overexpression of PfPIS/ScPIS hybrids in ScPIS1-deficient Saccharomyces cerevisiae mutant strain BY4743, analysis by enzyme assay (30°C, yeast membrane pellet, in presence of 18 mM octyl-beta-D-glucopyranoside) or lipid incorporation and extraction | Plasmodium falciparum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 23735, calculated | Plasmodium knowlesi |
| ? | x * 23924, calculated | Plasmodium falciparum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CDP-diacylglacerol: myo-inositol 3-phosphatidyl-transferase | - |
Plasmodium knowlesi |
| CDP-diacylglacerol: myo-inositol 3-phosphatidyl-transferase | - |
Plasmodium falciparum |
| PfPIS | - |
Plasmodium falciparum |
| phosphatidyinositol synthase | - |
Plasmodium knowlesi |
| phosphatidyinositol synthase | - |
Plasmodium falciparum |
| PI synthase | - |
Plasmodium knowlesi |
| PI synthase | - |
Plasmodium falciparum |
| PIS | - |
Plasmodium knowlesi |
| PIS | - |
Plasmodium falciparum |
| PkPIS | - |
Plasmodium knowlesi |
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