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Literature summary for 2.7.8.11 extracted from
- Phosphatidylinositol synthase from mammalian tissues (1997), Biochim. Biophys. Acta, 1348, 179-186.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cDNA cloned by functional complementation of a Saccharomyces cerevisiae mutant | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Bos taurus |  |
| Ca2+ | - |
Cavia porcellus | |
| Ca2+ | - |
Homo sapiens | |
| Ca2+ | - |
Oryctolagus cuniculus | |
| Ca2+ | - |
Rattus norvegicus | |
| CDP-diacylglycerol | above 0.5 mM | Homo sapiens | |
| CDP-diacylglycerol | above 0.4 mM | Rattus norvegicus | |
| diphosphate | - |
Homo sapiens | |
| hexachlorocyclohexane | inhibition is non-competitive and not specific for inositol processing enzymes | Homo sapiens | |
| additional information | AMP CMP, GMP, UMP, TMP, TDP, and GDP are not inhibitory, triphosphates are even more inhibitory than diphosphates; no inhibition with 2-deoxy-CDP-diacylglycerol | Homo sapiens | |
| phosphate | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0095 | - |
CDP-diacylglycerol | - |
Rattus norvegicus | |
| 0.036 | - |
CDP-diacylglycerol | - |
Homo sapiens | |
| 0.045 | - |
2-deoxy-CDP-diacylglycerol | - |
Homo sapiens | |
| 0.28 | - |
myo-inositol | - |
Homo sapiens | |
| 1.3 | - |
myo-inositol | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | - |
Homo sapiens | 5783 | - |
| endoplasmic reticulum | - |
Oryctolagus cuniculus | 5783 | - |
| endoplasmic reticulum | localized to both the rough and the smooth endoplasmic reticulum | Rattus norvegicus | 5783 | - |
| Golgi apparatus | - |
Homo sapiens | 5794 | - |
| Golgi apparatus | - |
Rattus norvegicus | 5794 | - |
| membrane | - |
Gallus gallus | 16020 | - |
| membrane | - |
Cavia porcellus | 16020 | - |
| membrane | membrane-bound | Homo sapiens | 16020 | - |
| membrane | membrane-bound | Rattus norvegicus | 16020 | - |
| membrane | membrane-bound | Saccharomyces cerevisiae | 16020 | - |
| microsome | - |
Cavia porcellus | - |
- |
| microsome | - |
Homo sapiens | - |
- |
| microsome | - |
Rattus norvegicus | - |
- |
| microsome | - |
Saccharomyces cerevisiae | - |
- |
| microsome | - |
Bos taurus | - |
- |
| microsome | - |
Oryctolagus cuniculus | - |
- |
| plasma membrane | - |
Rattus norvegicus | 5886 | - |
| plasma membrane | - |
Saccharomyces cerevisiae | 5886 | - |
| plasma membrane | proximal tubule cells | Oryctolagus cuniculus | 5886 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Cavia porcellus | |
| Mg2+ | - |
Homo sapiens | |
| Mg2+ | - |
Rattus norvegicus | |
| Mg2+ | - |
Saccharomyces cerevisiae | |
| Mg2+ | - |
Bos taurus | |
| Mg2+ | - |
Oryctolagus cuniculus | |
| Mn2+ | - |
Cavia porcellus | |
| Mn2+ | - |
Homo sapiens | |
| Mn2+ | - |
Rattus norvegicus | |
| Mn2+ | - |
Saccharomyces cerevisiae | |
| Mn2+ | - |
Bos taurus | |
| Mn2+ | - |
Oryctolagus cuniculus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 21000 | - |
? * 21000, SDS-PAGE | Rattus norvegicus |
| 23610 | - |
- |
Rattus norvegicus |
| 24000 | - |
SDS-PAGE | Homo sapiens |
| 34000 | - |
- |
Rattus norvegicus |
| 34000 | - |
- |
Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CDP-diacylglycerol + myo-inositol | Gallus gallus | - |
CMP + phosphatidyl-1D-myo-inositol | - |
r | |
| CDP-diacylglycerol + myo-inositol | Meleagris gallopavo | - |
CMP + phosphatidyl-1D-myo-inositol | - |
r | |
| CDP-diacylglycerol + myo-inositol | Cavia porcellus | - |
CMP + phosphatidyl-1D-myo-inositol | - |
r | |
| CDP-diacylglycerol + myo-inositol | Homo sapiens | - |
CMP + phosphatidyl-1D-myo-inositol | - |
r | |
| CDP-diacylglycerol + myo-inositol | Saccharomyces cerevisiae | - |
CMP + phosphatidyl-1D-myo-inositol | - |
r | |
| CDP-diacylglycerol + myo-inositol | Oryctolagus cuniculus | - |
CMP + phosphatidyl-1D-myo-inositol | - |
r | |
| CDP-diacylglycerol + myo-inositol | Rattus norvegicus | enzyme catalyzes the final step of de novo synthesis of phosphatidylinositol | CMP + phosphatidyl-1D-myo-inositol | - |
r | |
| CDP-diacylglycerol + myo-inositol | Bos taurus | enzyme catalyzes the final step of de novo synthesis of phosphatidylinositol | CMP + phosphatidyl-1D-myo-inositol | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Cavia porcellus | - |
guinea pig | - |
| Gallus gallus | - |
chicken | - |
| Homo sapiens | - |
human | - |
| Meleagris gallopavo | - |
turkey | - |
| Oryctolagus cuniculus | - |
- |
- |
| Rattus norvegicus | - |
rat | - |
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
- |
Rattus norvegicus |
- |
Saccharomyces cerevisiae |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Gallus gallus | - |
| brain | - |
Cavia porcellus | - |
| brain | - |
Rattus norvegicus | - |
| brain | - |
Bos taurus | - |
| cerebral cortex | - |
Oryctolagus cuniculus | - |
| erythrocyte | - |
Meleagris gallopavo | - |
| glioma cell | - |
Oryctolagus cuniculus | - |
| heart | - |
Gallus gallus | - |
| heart | - |
Cavia porcellus | - |
| kidney | - |
Cavia porcellus | - |
| liver | - |
Gallus gallus | - |
| liver | - |
Cavia porcellus | - |
| liver | - |
Rattus norvegicus | - |
| lung | - |
Cavia porcellus | - |
| lung | - |
Oryctolagus cuniculus | - |
| neuroblastoma cell | - |
Oryctolagus cuniculus | - |
| pancreas | - |
Cavia porcellus | - |
| pituitary gland tumor cell | GH3 tumour | Rattus norvegicus | - |
| placenta | - |
Homo sapiens | - |
| spleen | - |
Cavia porcellus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.8 | - |
- |
Rattus norvegicus |
| 34 | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-deoxy-CDP-diacylglycerol + myo-inositol | optimal activity is 25% higher then with CDP-diacylglycerol | Homo sapiens | CMP + ? | - |
r | |
| CDP-diacylglycerol + myo-inositol | - |
Gallus gallus | CMP + phosphatidyl-1D-myo-inositol | - |
r | |
| CDP-diacylglycerol + myo-inositol | - |
Meleagris gallopavo | CMP + phosphatidyl-1D-myo-inositol | - |
r | |
| CDP-diacylglycerol + myo-inositol | - |
Cavia porcellus | CMP + phosphatidyl-1D-myo-inositol | - |
r | |
| CDP-diacylglycerol + myo-inositol | - |
Homo sapiens | CMP + phosphatidyl-1D-myo-inositol | - |
r | |
| CDP-diacylglycerol + myo-inositol | - |
Rattus norvegicus | CMP + phosphatidyl-1D-myo-inositol | - |
r | |
| CDP-diacylglycerol + myo-inositol | - |
Saccharomyces cerevisiae | CMP + phosphatidyl-1D-myo-inositol | - |
r | |
| CDP-diacylglycerol + myo-inositol | - |
Bos taurus | CMP + phosphatidyl-1D-myo-inositol | - |
r | |
| CDP-diacylglycerol + myo-inositol | - |
Oryctolagus cuniculus | CMP + phosphatidyl-1D-myo-inositol | - |
r | |
| CDP-diacylglycerol + myo-inositol | enzyme catalyzes the final step of de novo synthesis of phosphatidylinositol | Rattus norvegicus | CMP + phosphatidyl-1D-myo-inositol | - |
r | |
| CDP-diacylglycerol + myo-inositol | enzyme catalyzes the final step of de novo synthesis of phosphatidylinositol | Bos taurus | CMP + phosphatidyl-1D-myo-inositol | - |
r | |
| additional information | - |
Saccharomyces cerevisiae | ? | - |
? | |
| additional information | UDP-diacylglycerol, ADP-diacylglycerol, and GDP-diacylglycerol are inactive as substrates | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | - |
Saccharomyces cerevisiae |
| ? | ? * 21000, SDS-PAGE | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | 9 | - |
Bos taurus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
diphosphate | - |
Homo sapiens | |
| 0.8 | - |
phosphate | - |
Homo sapiens | |
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