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Literature summary for 2.7.8.11 extracted from
- Candida albicans phosphatidylinositol synthase has common features with both Saccharomyces cerevisiae and mammalian phosphatidylinositol synthases (1996), Yeast, 12, 449-456.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | one of the major human fungal pathogens, expanded knowledge about essential metabloic processes and enzymes in this pathogenic fungus can pave the way for the development of more effective treatments | Candida albicans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Candida albicans |  |
| ATP | - |
Candida albicans | |
| CaCl2 | - |
Candida albicans | |
| CDP | - |
Candida albicans | |
| CTP | - |
Candida albicans | |
| diphosphate | most efficient inhibitor | Candida albicans | |
| GTP | - |
Candida albicans | |
| additional information | no substrate inhibition with 2-deoxy-CDPdiacylglycerol, CMP and to a lesser extent AMP shows low inhibition of the enzyme | Candida albicans | |
| phosphate | - |
Candida albicans | |
| Triton X-100 | above 1% | Candida albicans | |
| TTP | - |
Candida albicans | |
| UDP | - |
Candida albicans | |
| UTP | - |
Candida albicans | |
| ZnCl2 | - |
Candida albicans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.029 | - |
2-deoxy-CDP-diacylglycerol | pH 9.0, 37°C | Candida albicans | |
| 0.036 | - |
CDP-diacylglycerol | pH 9.0, 37°C | Candida albicans | |
| 0.55 | - |
myo-inositol | pH 9.0, 30°C | Candida albicans | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | membrane-bound | Candida albicans | 16020 | - |
| microsome | - |
Candida albicans | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | reaction and reverse reaction dependent on either Mg2+ or Mn2+ | Candida albicans | |
| Mn2+ | - |
Candida albicans | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CDP-diacylglycerol + myo-inositol | Candida albicans | - |
CMP + phosphatidyl-1D-myo-inositol | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Candida albicans | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.001 | 0.002 | - |
Candida albicans |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, frozen in liquid N2 and stored, no loss of activity is detected during storage for up to 2 months | Candida albicans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-deoxy-CDP-diacylglycerol + myo-inositol | maximal activity only 36% of the optimal activity reached with CDP-diacylglycerol | Candida albicans | CMP + ? | - |
r | |
| CDP-diacylglycerol + myo-inositol | - |
Candida albicans | CMP + phosphatidyl-1D-myo-inositol | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Candida albicans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 50 | thermal denaturation at higher temperatures, half-life at 50°C 30 min | Candida albicans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
highest activity in Tris buffer, only 43% of the activity in glycine/NaOH buffer at pH 9.5 | Candida albicans |
| 9.5 | - |
glycine NaOH buffer | Candida albicans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 10.5 | - |
Candida albicans |
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