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Literature summary for 2.7.8.11 extracted from

  • Parries, G.S.; Hokin-Neaverson, M.
    Phosphatidylinositol synthase from canine pancreas: solubilization by n-octyl glucopyranoside and stabilization by manganese (1984), Biochemistry, 23, 4785-4791.
    View publication on PubMed
Search for more literature for 2.7.8.11

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018
-
 CDP-diacylglycerol pH 8.5, 37°C Canis lupus familiaris
0.76
-
 myo-inositol pH 8.5, 37°C Canis lupus familiaris

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
 Canis lupus familiaris 5783
-
membrane membrane-bound Canis lupus familiaris 16020
-
microsome
-
 Canis lupus familiaris
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Canis lupus familiaris
Mn2+
-
 Canis lupus familiaris
additional information replacement of Mn2+ or Mg2+ with 1 mM Co2+ give approximately 10% of maximal activity, 1 mM Zn2+, Ni2+, Cu2+, Ca2+ and Fe2+ does not activate the enzyme Canis lupus familiaris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 ? * 30000 + ? * 60000, SDS-PAGE Canis lupus familiaris
60000
-
 ? * 30000 + ? * 60000, SDS-PAGE Canis lupus familiaris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CDP-diacylglycerol + myo-inositol Canis lupus familiaris enzyme catalyzes the final step of de novo synthesis of phosphatidylinositol CMP + phosphatidyl-1D-myo-inositol
-
 r
CDP-diacylglycerol + myo-inositol Canis lupus familiaris enzyme plays a role in resynthesis of phosphatidylinositol during agonist-stimulated inositol-phospholipid metabolism CMP + phosphatidyl-1D-myo-inositol
-
 r

Organism

Organism UniProt Comment Textmining
Canis lupus familiaris
-
 dog
-
Canis lupus familiaris
-
 canine
-

Purification (Commentary)

Purification (Comment) Organism
partially Canis lupus familiaris

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
 Canis lupus familiaris
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.03
-
-
 Canis lupus familiaris
0.29
-
-
 Canis lupus familiaris

Storage Stability

Storage Stability Organism
-80°C, no appreciable loss of PI synthase activity detected for up to at least 6 months Canis lupus familiaris
4°C, 67% of the activity remains after 3 days Canis lupus familiaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CDP-diacylglycerol + myo-inositol
-
 Canis lupus familiaris CMP + phosphatidyl-1D-myo-inositol
-
 r
CDP-diacylglycerol + myo-inositol enzyme catalyzes the final step of de novo synthesis of phosphatidylinositol Canis lupus familiaris CMP + phosphatidyl-1D-myo-inositol
-
 r
CDP-diacylglycerol + myo-inositol enzyme plays a role in resynthesis of phosphatidylinositol during agonist-stimulated inositol-phospholipid metabolism Canis lupus familiaris CMP + phosphatidyl-1D-myo-inositol
-
 r

Subunits

Subunits Comment Organism
? ? * 30000 + ? * 60000, SDS-PAGE Canis lupus familiaris

Synonyms

Synonyms Comment Organism
PI synthase
-
 Canis lupus familiaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7 pH optimum in reverse direction Canis lupus familiaris
8.5 9 optimum in the forward direction Canis lupus familiaris