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Literature summary for 2.7.8.1 extracted from
- 1,2-Diacylglycerol: CDPcholine cholinephosphotransferase (1981), Methods Enzymol., 71, 536-546.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| stable if treated with 0.5% Triton X-100 | Rattus norvegicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.022 | - |
CDP-ethanolamine | 30°C, pH 8.0 | Rattus norvegicus |  |
| 0.063 | - |
diacylglycerol | 30°C, pH 8.0 | Rattus norvegicus | |
| 0.14 | - |
CMP | 30°C, pH 8.0 | Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | cytoplasmic surface | Rattus norvegicus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | can partially replace Mn2+ in activation | Rattus norvegicus | |
| Mn2+ | required | Rattus norvegicus | |
| Mn2+ | much more effective than Mg2+ | Rattus norvegicus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| below -20°C, stable for more than 1 month, microsomal preparation | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CDP-ethanolamine + 1,2-diacylglycerol | - |
Rattus norvegicus | CMP + a phosphatidylethanolamine | - |
r | |
| additional information | several 1-oleoyl-2-saturated types of diacylglycerol are effectively used | Rattus norvegicus | ? | - |
? | |
| additional information | fully saturated diacylglycerols like dipalmitoylglycerol are poorly utilized | Rattus norvegicus | ? | - |
? | |
| additional information | 1-acyl-2-oleoylglycerols ranging from 1-lauroyl to 1-heptadecanoyl species: 1-heptadecanoyl and 1-stearoyl species used most actively | Rattus norvegicus | ? | - |
? | |
| phosphatidylcholine + CMP | - |
Rattus norvegicus | CDP-choline + diacylglycerol | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
temperature dependence | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.5 | - |
Rattus norvegicus |
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Release 2023.1 (January 2023)
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