BRENDA - Enzyme Database show
show all sequences of 2.7.7.B5

The rhodanese domain of ThiI is both necessary and sufficient for synthesis of the thiazole moiety of thiamine in Salmonella enterica

Martinez-Gomez, N.C.; Palmer, L.D.; Vivas, E.; Roach, P.L.; Downs, D.M.; J. Bacteriol. 193, 4582-4587 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Salmonella enterica
Engineering
Amino acid exchange
Commentary
Organism
C207S
using a complementation assay it is shown that mutant is required for 4-thiouridine synthesis but not for thiazole synthesis
Salmonella enterica
C344S
using a complementation assay it is shown that mutant is required for 4-thiouridine synthesis but not for thiazole synthesis
Salmonella enterica
C456S
using a complementation assay it is shown that mutant is required for 4-thiouridine synthesis and for thiazole synthesis
Salmonella enterica
D189A
using a complementation assay it is shown that mutant is required for 4-thiouridine synthesis but not for thiazole synthesis
Salmonella enterica
K321M
using a complementation assay it is shown that mutant is required for 4-thiouridine synthesis but not for thiazole synthesis
Salmonella enterica
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Salmonella enterica
-
-
-
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Salmonella enterica
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
C207S
using a complementation assay it is shown that mutant is required for 4-thiouridine synthesis but not for thiazole synthesis
Salmonella enterica
C344S
using a complementation assay it is shown that mutant is required for 4-thiouridine synthesis but not for thiazole synthesis
Salmonella enterica
C456S
using a complementation assay it is shown that mutant is required for 4-thiouridine synthesis and for thiazole synthesis
Salmonella enterica
D189A
using a complementation assay it is shown that mutant is required for 4-thiouridine synthesis but not for thiazole synthesis
Salmonella enterica
K321M
using a complementation assay it is shown that mutant is required for 4-thiouridine synthesis but not for thiazole synthesis
Salmonella enterica
Other publictions for EC 2.7.7.B5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725262
Martinez-Gomez
The rhodanese domain of ThiI i ...
Salmonella enterica
J. Bacteriol.
193
4582-4587
2011
-
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1
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5
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-
-
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1
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-
-
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-
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1
-
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5
-
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-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
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710548
Tanaka
Deduced RNA binding mechanism ...
Escherichia coli
RNA
15
1498-1506
2009
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1
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1
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-
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1
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1
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1
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691789
You
Direct evidence that thil is a ...
Escherichia coli
ChemBioChem
9
1879-1882
2008
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-
1
-
2
-
-
2
-
-
-
2
-
1
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1
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5
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1
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2
1
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1
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2
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2
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2
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1
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5
-
1
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2
1
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694560
Naumann
-
Versuche zur Strukturaufklärun ...
Escherichia coli, Thermotoga maritima
PH. D. Thesis Universität Göttingen
2005
0000
2005
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2
1
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2
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2
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2
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1
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2
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