BRENDA - Reference to 2.7.7.B5; Id = 710548

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Escherichia coli

additional information

construction of two truncated forms of ThiI containing the N-terminal domain including NFLD and THUMP, and the C-terminal domain including the PP-loop and RLD domains, respectively. The N-domain can bind with both tRNAPhe and TPHE39A and recognizes the acceptor-stem region, whereas the C-domain cannot. The C-domain also affects RNA binding by its enthalpically favorable, but entropically unfavorable, contribution. The C-domain induces a conformation change in tRNAPhe

Escherichia coli

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tRNA-uridine + ATP = adenylated-trNA-uridine + diphosphate

RNA binding mechanism of ThiI in which the N-terminal domain recognizes the acceptor-stem region and the C-terminal region causes a conformational change of RNA

Escherichia coli

ATP + TPHE39A

truncated tRNA consisting of 39 nucleotides derived from tRNAPhe, minimal RNA substrate for modification by ThiI. The crystal structure of TPHE39A shows that base pairs in the T-stem are almost completely disrupted, although those in the acceptor-stem are preserved. ThiI can efficiently bind with not only tRNAPhe but also TPHE39A

710548

Escherichia coli

adenylated TPHE39A + diphosphate

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?

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Escherichia coli

additional information

construction of two truncated forms of ThiI containing the N-terminal domain including NFLD and THUMP, and the C-terminal domain including the PP-loop and RLD domains, respectively. The N-domain can bind with both tRNAPhe and TPHE39A and recognizes the acceptor-stem region, whereas the C-domain cannot. The C-domain also affects RNA binding by its enthalpically favorable, but entropically unfavorable, contribution. The C-domain induces a conformation change in tRNAPhe

Escherichia coli

ATP + TPHE39A

truncated tRNA consisting of 39 nucleotides derived from tRNAPhe, minimal RNA substrate for modification by ThiI. The crystal structure of TPHE39A shows that base pairs in the T-stem are almost completely disrupted, although those in the acceptor-stem are preserved. ThiI can efficiently bind with not only tRNAPhe but also TPHE39A

710548

Escherichia coli

adenylated TPHE39A + diphosphate

-

?

725262

Martinez-Gomez

The rhodanese domain of ThiI i ...

Salmonella enterica

J. Bacteriol.

193

4582-4587

2011

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1

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5

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1

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1

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1

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5

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710548

Tanaka

Deduced RNA binding mechanism ...

Escherichia coli

RNA

15

1498-1506

2009

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1

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1

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1

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1

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1

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1

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1

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1

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691789

You

Direct evidence that thil is a ...

Escherichia coli

ChemBioChem

9

1879-1882

2008

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1

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2

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2

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2

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1

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1

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5

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1

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2

1

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1

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2

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2

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2

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1

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5

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1

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2

1

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694560

Naumann

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Versuche zur Strukturaufkläru ...

Escherichia coli, Thermotoga maritima

PH. D. Thesis Universität Göttingen

2005

0000

2005

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2

1

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2

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2

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2

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2

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1

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2

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Deduced RNA binding mechanism of ThiI based on structural and binding analyses of a minimal RNA ligand

Data extracted from this reference: