Activating Compound | Comment | Organism | Structure |
---|---|---|---|
heat-stable soluble activator | requirement for a heat-stable soluble activator present in bacterial extracts | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
bis-Tris-propane | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | varying the ADP concentration does not yield Michealis-Menten kinetics | Escherichia coli | |
4.2 | - |
thiamine diphosphate | pH 6.5, 37°C, cosubstrate: ADP | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 5 mM, requirement for divalent metal ions, Mn2+ is most efficient | Escherichia coli | |
Mn2+ | 5 mM, requirement for divalent metal ions, Mg2+ is 70% less effective than Mg2+ | Escherichia coli | |
additional information | 5 mM Ca2+ has no effect on activity | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
thiamine diphosphate + ADP | Escherichia coli | biosynthesis of adenosine thiamine triphosphate. Adenosine thiamine triphosphate appears to be a signal produced in bacteria in response to some form of cellular stress | adenosine thiamine triphosphate + phosphate | - |
? | |
thiamine diphosphate + ATP | Escherichia coli | biosynthesis of adenosine thiamine triphosphate. Adenosine thiamine triphosphate appears to be a signal produced in bacteria in response to some form of cellular stress | adenosine thiamine triphosphate + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00008 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
thiamine diphosphate + ADP | biosynthesis of adenosine thiamine triphosphate. Adenosine thiamine triphosphate appears to be a signal produced in bacteria in response to some form of cellular stress | Escherichia coli | adenosine thiamine triphosphate + phosphate | - |
? | |
thiamine diphosphate + ADP | the enzyme does not accept a thiamine phopshate other than thiamine diphosphate as substrate | Escherichia coli | adenosine thiamine triphosphate + phosphate | - |
? | |
thiamine diphosphate + ATP | biosynthesis of adenosine thiamine triphosphate. Adenosine thiamine triphosphate appears to be a signal produced in bacteria in response to some form of cellular stress | Escherichia coli | adenosine thiamine triphosphate + diphosphate | - |
? | |
thiamine diphosphate + ATP | the enzyme does not accept a thiamine phopshate other than thiamine diphosphate as substrate | Escherichia coli | adenosine thiamine triphosphate + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ThDP adenylyl transferase | - |
Escherichia coli |
thiamine diphosphate adenylyl transferase | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | - |
Escherichia coli |