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Literature summary for 2.7.7.B24 extracted from

  • Ming, Z.; Wang, W.; Xie, Y.; Ding, P.; Chen, Y.; Jin, D.; Sun, Y.; Xia, B.; Yan, L.; Lou, Z.
    Crystal structure of the novel di-nucleotide cyclase from Vibrio cholerae (DncV) responsible for synthesizing a hybrid cyclic GMP-AMP (2014), Cell Res., 24, 1270-1273.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.B24

Activating Compound

Activating Compound Comment Organism Structure
additional information no activitation by binding to DNa required Vibrio cholerae O1

Crystallization (Commentary)

Crystallization (Comment) Organism
wild-type and mutant D131N/D133N. Catalytic residues are D131 and D133 in strand beta1, and D193 in strand beta5, and the G[G/S]xx motif is defined by residues G113-S114-F115-Q116 in a well-defined loop linking helix alpha5 and strand beta1 Vibrio cholerae O1

Protein Variants

Protein Variants Comment Organism
D131N mutation in conserved acidic residue, loss of activity Vibrio cholerae O1
D131N/D133N mutation in conserved acidic residues, loss of activity Vibrio cholerae O1
D133N mutation in conserved acidic residue, loss of activity Vibrio cholerae O1
D193N mutation in conserved acidic residue, loss of activity Vibrio cholerae O1
K287A mutation in residue that participates in the interaction with diphosphate, impairs the ability to catalyze cyclic di-GMP formation Vibrio cholerae O1

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + GTP Vibrio cholerae O1
-
 2 diphosphate + cyclic Ap(3'-5')Gp
-
 ?
ATP + GTP Vibrio cholerae O1 ATCC 39315
-
 2 diphosphate + cyclic Ap(3'-5')Gp
-
 ?
GTP + ATP Vibrio cholerae O1
-
 2 diphosphate + cyclic Gp(3'-5')A in presence of a mixture of GTP and ATP cGAMP is the dominant product, with little cyclic di-AMP and cyclic di-GMP generated ?
GTP + ATP Vibrio cholerae O1 ATCC 39315
-
 2 diphosphate + cyclic Gp(3'-5')A in presence of a mixture of GTP and ATP cGAMP is the dominant product, with little cyclic di-AMP and cyclic di-GMP generated ?
GTP + GTP Vibrio cholerae O1
-
 2 diphosphate + cyclic Gp(3'-5')G
-
 ?
GTP + GTP Vibrio cholerae O1 ATCC 39315
-
 2 diphosphate + cyclic Gp(3'-5')G
-
 ?

Organism

Organism UniProt Comment Textmining
Vibrio cholerae O1 Q9KVG7
-
-
Vibrio cholerae O1 ATCC 39315 Q9KVG7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + GTP
-
 Vibrio cholerae O1 2 diphosphate + cyclic Ap(3'-5')Gp
-
 ?
ATP + GTP
-
 Vibrio cholerae O1 2 diphosphate + cyclic Ap(3'-5')Gp a mixture of GTP and ATP triggers the synthesis of cGAMP as the dominant product, with little c-di-AMP and c-di-GMP generated ?
ATP + GTP
-
 Vibrio cholerae O1 ATCC 39315 2 diphosphate + cyclic Ap(3'-5')Gp
-
 ?
ATP + GTP
-
 Vibrio cholerae O1 ATCC 39315 2 diphosphate + cyclic Ap(3'-5')Gp a mixture of GTP and ATP triggers the synthesis of cGAMP as the dominant product, with little c-di-AMP and c-di-GMP generated ?
GTP + ATP
-
 Vibrio cholerae O1 2 diphosphate + cyclic Gp(3'-5')A in presence of a mixture of GTP and ATP cGAMP is the dominant product, with little cyclic di-AMP and cyclic di-GMP generated ?
GTP + ATP
-
 Vibrio cholerae O1 ATCC 39315 2 diphosphate + cyclic Gp(3'-5')A in presence of a mixture of GTP and ATP cGAMP is the dominant product, with little cyclic di-AMP and cyclic di-GMP generated ?
GTP + GTP
-
 Vibrio cholerae O1 2 diphosphate + cyclic Gp(3'-5')G
-
 ?
GTP + GTP
-
 Vibrio cholerae O1 ATCC 39315 2 diphosphate + cyclic Gp(3'-5')G
-
 ?