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Literature summary for 2.7.7.B19 extracted from

  • Zhang, Z.; Tsujimura, M.; Akutsu, J.; Sasaki, M.; Tajima, H.; Kawarabayasi, Y.
    Identification of an extremely thermostable enzyme with dual sugar-1-phosphate nucleotidylyltransferase activities from an acidothermophilic archaeon, Sulfolobus tokodaii strain 7 (2005), J. Biol. Chem., 280, 9698-9705.
    View publication on PubMed
Search for more literature for 2.7.7.B19

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, wild-type and truncated enzyme form lacking the 170-residues C-terminal domain Sulfurisphaera tokodaii

Organism

Organism UniProt Comment Textmining
Sulfurisphaera tokodaii Q975F9
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Sulfurisphaera tokodaii 7 Q975F9
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-

Purification (Commentary)

Purification (Comment) Organism
-
 Sulfurisphaera tokodaii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.62
-
 pH 7.5, 80°C, substrates: dGTP + alpha-D-glucose 1-phosphate Sulfurisphaera tokodaii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dGTP + alpha-D-glucose 1-phosphate maximal velocity of the reverse reaction is 11.4fold of the maximal velocity of the forward reaction. The multifunctional enzyme also shows activity with UTP + alpha-D-glucose 1-phosphate (EC 2.7.7.9, UTP-glucose-1-phosphate uridylyltransferase), UTP + N-acetylglucosamine 1-phosphate (EC 2.7.7.23, UDP-N-acetylglucosamine diphosphorylase), dTTP + alpha-D-glucose 1-phosphate (EC 2.7.7.24, glucose-1-phosphate thymidylyltransferase), dTTP + N-acetyl-alpha-D-glucosamine 1-phosphate (N-acetylglucosamine 1-phosphate thymidylyltransferase), dCTP + alpha-D-glucose 1-phosphate (glucose-1-phosphate cytidylyltransferase), dATP + alpha-D-glucose 1-phosphate (glucose-1-phosphate adenylyltransferase). No activity with: alpha-D-glucose 1-phosphate + dATP, alpha-D-glucose 1-phosphate + dCTP, alpha-D-glucose 1-phosphate + dGTP, UTP + alpha-D-mannose1-phosphate, UTP + alpha-D-galactose 1-phosphate, UTP + alpha-D-glucosamine 1-phosphate Sulfurisphaera tokodaii diphosphate + dGDP-alpha-D-glucose
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 ?
dGTP + alpha-D-glucose 1-phosphate maximal velocity of the reverse reaction is 11.4fold of the maximal velocity of the forward reaction. The multifunctional enzyme also shows activity with UTP + alpha-D-glucose 1-phosphate (EC 2.7.7.9, UTP-glucose-1-phosphate uridylyltransferase), UTP + N-acetylglucosamine 1-phosphate (EC 2.7.7.23, UDP-N-acetylglucosamine diphosphorylase), dTTP + alpha-D-glucose 1-phosphate (EC 2.7.7.24, glucose-1-phosphate thymidylyltransferase), dTTP + N-acetyl-alpha-D-glucosamine 1-phosphate (N-acetylglucosamine 1-phosphate thymidylyltransferase), dCTP + alpha-D-glucose 1-phosphate (glucose-1-phosphate cytidylyltransferase), dATP + alpha-D-glucose 1-phosphate (glucose-1-phosphate adenylyltransferase). No activity with: alpha-D-glucose 1-phosphate + dATP, alpha-D-glucose 1-phosphate + dCTP, alpha-D-glucose 1-phosphate + dGTP, UTP + alpha-D-mannose1-phosphate, UTP + alpha-D-galactose 1-phosphate, UTP + alpha-D-glucosamine 1-phosphate Sulfurisphaera tokodaii 7 diphosphate + dGDP-alpha-D-glucose
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 ?
dGTP + alpha-D-glucose 1-phosphate maximal velocity of the reverse reaction is 11.4fold of the maximal velocity of the forward reaction. The multifunctional enzyme also shows activity with UTP + alpha-D-glucose 1-phosphate (EC 2.7.7.9, UTP-glucose-1-phosphate uridylyltransferase), UTP + N-acetylglucosamine 1-phosphate (EC 2.7.7.23, UDP-N-acetylglucosamine diphosphorylase), dTTP + alpha-D-glucose 1-phosphate (EC 2.7.7.24, glucose-1-phosphate thymidylyltransferase), dTTP + N-acetyl-alpha-D-glucosamine 1-phosphate (N-acetylglucosamine 1-phosphate thymidylyltransferase), dCTP + alpha-D-glucose 1-phosphate (glucose-1-phosphate cytidylyltransferase), dATP + alpha-D-glucose 1-phosphate (glucose-1-phosphate adenylyltransferase). No activity with: alpha-D-glucose 1-phosphate + dATP, alpha-D-glucose 1-phosphate + dCTP, alpha-D-glucose 1-phosphate + dGTP, UTP + alpha-D-mannose1-phosphate, UTP + alpha-D-galactose 1-phosphate, UTP + alpha-D-glucosamine 1-phosphate Sulfurisphaera tokodaii DSM 16993 diphosphate + dGDP-alpha-D-glucose
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 ?

Synonyms

Synonyms Comment Organism
ST0452 locus name Sulfurisphaera tokodaii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
 assay at Sulfurisphaera tokodaii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Sulfurisphaera tokodaii