Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required | Thermococcus nautili |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus nautili | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | primase PolpTN2 is able to prime the synthesis of dsDNA by Taq DNA polymerase and by DNA polymerase PolB encoded by the chromosome of Thermococcus nautili using M13mp18ssDNA as a template, method development and evaluation, overview. Mechanism of template-free synthesis of seeds by PolB and PolpTN2 from dNTP substrates | Thermococcus nautili | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PolpTN2 | - |
Thermococcus nautili |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
template-free synthesis activity | Thermococcus nautili |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
delayed kinetics of the reaction at low temperature | Thermococcus nautili |
37 | - |
coupled assay with DNA polymerase B | Thermococcus nautili |
68 | 80 | activity range of template?free synthesis, which is highly temperature-dependent | Thermococcus nautili |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.9 | - |
assay at | Thermococcus nautili |
General Information | Comment | Organism |
---|---|---|
metabolism | helicase is not essential but accelerates the reaction. HelpTN2 is a helicase of the SF1 family also encoded by pTN2 who enhances the PolpTN2-primed synthesis of dsDNA by PolB using M13mp18 as a template | Thermococcus nautili |
physiological function | DNA primase PolpTN2 and DNA polymerase PolB are essential for synthesis of dsDNA from deoxynucleotide triphosphates by producing an oligonucleotide primer. The first step of template-free DNA synthesis may involve the creation of seeds by PolpTN2 primase, which are subsequently amplified in synergy with PolB. Template?free synthesis is highly temperature?dependent | Thermococcus nautili |