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Literature summary for 2.7.7.B16 extracted from

  • Beguin, P.; Gill, S.; Charpin, N.; Forterre, P.
    Synergistic template-free synthesis of dsDNA by Thermococcus nautili primase PolpTN2, DNA polymerase PolB, and pTN2 helicase (2015), Extremophiles, 19, 69-76.
    View publication on PubMed

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Thermococcus nautili

Organism

Organism UniProt Comment Textmining
Thermococcus nautili
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information primase PolpTN2 is able to prime the synthesis of dsDNA by Taq DNA polymerase and by DNA polymerase PolB encoded by the chromosome of Thermococcus nautili using M13mp18ssDNA as a template, method development and evaluation, overview. Mechanism of template-free synthesis of seeds by PolB and PolpTN2 from dNTP substrates Thermococcus nautili ?
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?

Synonyms

Synonyms Comment Organism
PolpTN2
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Thermococcus nautili

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
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template-free synthesis activity Thermococcus nautili

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
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delayed kinetics of the reaction at low temperature Thermococcus nautili
37
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coupled assay with DNA polymerase B Thermococcus nautili
68 80 activity range of template?free synthesis, which is highly temperature-dependent Thermococcus nautili

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
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assay at Thermococcus nautili

General Information

General Information Comment Organism
metabolism helicase is not essential but accelerates the reaction. HelpTN2 is a helicase of the SF1 family also encoded by pTN2 who enhances the PolpTN2-primed synthesis of dsDNA by PolB using M13mp18 as a template Thermococcus nautili
physiological function DNA primase PolpTN2 and DNA polymerase PolB are essential for synthesis of dsDNA from deoxynucleotide triphosphates by producing an oligonucleotide primer. The first step of template-free DNA synthesis may involve the creation of seeds by PolpTN2 primase, which are subsequently amplified in synergy with PolB. Template?free synthesis is highly temperature?dependent Thermococcus nautili