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Literature summary for 2.7.7.B16 extracted from

  • Desogus, G.; Onesti, S.; Brick, P.; Rossi, M.; Pisani, F.M.
    Identification and characterization of a DNA primase from the hyperthermophilic archaeon Methanococcus jannaschii (1999), Nucleic Acids Res., 27, 4444-4450.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.B16

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Methanocaldococcus jannaschii

Metals/Ions

Metals/Ions Comment Organism Structure
K+ activity requires divalent cations such Mg2+, Mn2+ or Zn2+, and is additionally stimulated by the monovalent cation K+ Methanocaldococcus jannaschii
Mg2+ activity requires divalent cations such Mg2+, Mn2+ or Zn2+, and is additionally stimulated by the monovalent cation K+ Methanocaldococcus jannaschii
Mn2+ activity requires divalent cations such Mg2+, Mn2+ or Zn2+, and is additionally stimulated by the monovalent cation K+ Methanocaldococcus jannaschii
Zn2+ activity requires divalent cations such Mg2+, Mn2+ or Zn2+, and is additionally stimulated by the monovalent cation K+ Methanocaldococcus jannaschii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41800
-
 x * 41800, calculated from sequence Methanocaldococcus jannaschii

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii Q58249
-
-
Methanocaldococcus jannaschii JAL-1 Q58249
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NTP + n NTP synthesise oligoribonucleotides on various pyrimidine single-stranded DNA templates poly(dT) and poly(dC). The enzyme is almost completely inactive on a poly(dA) synthetic template Methanocaldococcus jannaschii N(pN)n + n diphosphate
-
 ?
NTP + n NTP synthesise oligoribonucleotides on various pyrimidine single-stranded DNA templates poly(dT) and poly(dC). The enzyme is almost completely inactive on a poly(dA) synthetic template Methanocaldococcus jannaschii JAL-1 N(pN)n + n diphosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 41800, calculated from sequence Methanocaldococcus jannaschii

Synonyms

Synonyms Comment Organism
Mjpri
-
 Methanocaldococcus jannaschii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 1 h, enzyme is stable Methanocaldococcus jannaschii
70
-
 10 min, 20% loss of activity Methanocaldococcus jannaschii
80
-
 half-life: 15 min Methanocaldococcus jannaschii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Methanocaldococcus jannaschii

pH Range

pH Minimum pH Maximum Comment Organism
7.5 8.5 pH 7.5: about 40% of maximal activity, pH 8.5: about 70% of maximal activity Methanocaldococcus jannaschii