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Literature summary for 2.7.7.B16 extracted from

  • Lao-Sirieix, S.H.; Nookala, R.K.; Roversi, P.; Bell, S.D.; Pellegrini, L.
    Structure of the heterodimeric core primase (2005), Nat. Struct. Mol. Biol., 12, 1137-1144.
    View publication on PubMed
Search for more literature for 2.7.7.B16

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion at 18°C, the structure provides the first three-dimensional description of the large subunit and its interaction with the small subunit Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
F164E(PriS) mutantion in small subunitPriS shows considerably weakened subunit association Saccharolobus solfataricus
F164G/I199K(PriS)_F142E/L163E(PriL) double mutations of F164G/I199K in small subunit PriS and F142E/L163E in large subunit PriL abolishes the PriS-PriL interaction Saccharolobus solfataricus
G165I(PriS) mutantion in small subunitPriS shows partial destabilization of the complex Saccharolobus solfataricus
additional information mutant primase lacking the zinc-binding motifs has higher activity compared with the wild-type enzyme, as well as a bias toward shorter products of 30–100 nucleotides Saccharolobus solfataricus
R84A/R85A(PriL) mutation in large subunit shows a marked reduction in the size and amount of RNA product synthesized Saccharolobus solfataricus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ contain one zinc ion in the small subunits. Residues Cys116, Cys119, Cys128 and Asp131 coordinate the zinc atom Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q9UWW1 and Q97Z83 Q9UWW1: large subunit, Q97Z83: small subunit
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Saccharolobus solfataricus P2 Q9UWW1 and Q97Z83 Q9UWW1: large subunit, Q97Z83: small subunit
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Purification (Commentary)

Purification (Comment) Organism
-
 Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
PriSL
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 Saccharolobus solfataricus
Sso core primase
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 Saccharolobus solfataricus